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Articles by Deepak Sharma
Total Records ( 4 ) for Deepak Sharma
  Deepak Sharma , Subodh Kumar , Sitangsu M. Deb , Abhijit Mitra , Saket K. Niranjan , Soumen Naskar and Arjava Sharma
  A fragment of 570 bp corresponding to exon 5 and 6 of integrin beta 2 (ITGB2) gene was amplified for screening D128G mutation in one hundred and fifty two buffaloes ( Bubalus bubalis ) which causes bovine leukocyte adhesion deficiency syndrome (BLAD) in cattle, as well as to ascertain polymorphism. TaqI PCR-RFLP revealed no such mutation thus indicating the absence of bubaline leukocyte adhesion deficiency (BuLAD) allele in animals under study. However, the polymorphism studies using MspI restriction enzyme revealed two genotypic patterns viz. AA pattern (bands of 293, 141, 105, and 31 bp) and BB pattern (bands of 293, 105, 77, 64, and 31 bp). The sequences of A and B alleles were submitted to the GenBank (EU853307 and AY821799).
  S.K. Singh , S. Mehra , S.K. Shukla , Vinay Kumar , A. Tiwari , M. Mehra , Giriraj Goyal , Jose Mathew and Deepak Sharma
  The MHC class I gene was amplified, cloned and sequenced in guinea fowl using the primers specific to BF2 gene in chicken. The nucleotide sequence of 571 bp partial CDS of BF2 gene includes 32 nucleotides of signal peptide (exon 1), complete α1 domain (270 nucleotides) and 269 nucleotides of α2 domain. For α1 and α domain no sequence variation was observed within guinea fowl sequences, however, high variability was observed within the other poultry species (15.93-28.03%) except chicken (7.95-9.16%). Between the guinea fowl and other poultry species, the α1 domain showed high nucleotide variability (29.26-43.70%). Among poultry species, guinea fowl showed least variability with chicken and maximum with duck. Among the substitutions, majorities were of non-synonymous (76.27%) with a ration of 1:3 between synonymous to non-synonymous substitutions. Guinea fowl showed lower genetic distances (Kimura 2-parameter) with chicken and quail (0.211-0.215), while with duck and goose, it showed higher genetic distances (0.343-0.350). Phylogenetic tree, based these genetic distances revealed two major clusters, comprising of guinea fowl, quail and chicken in one with guinea fowl as separate branch, while duck and goose in other.
  G. Goyal , V. Upmanyu , S.K. Singh , S.K. Shukla , S. Mehra , Vinay Kumar and Deepak Sharma
  Differential expression of IL-6 and IGF-II genes were studied in guinea fowl and broiler chicken using semi-quantitative analysis. A 219 bp fragment of IL-6 and 215 bp fragment of IGF-II were amplified in guinea fowl and broiler chicken using chicken specific primers. Semi-quantitative analysis revealed the adjusted Integral Density of 0.853 and 0.051 for IL-6 band in guinea fowl and broiler chicken respectively, revealing 16.62 fold higher IL-6 mRNA expression in LPS induced PBMCs from guinea fowl as compared to that from broiler. However, adjusted Integral Density of IGF-II band was 0.082 and 1.106 for IGF-II band in guinea fowl and broiler chicken respectively, which revealed 13.43 fold increase in IGF-II mRNA expression in LPS induced PBMCs in broiler chicken as compared to that in guinea fowl. Hence, guinea fowl showed higher expression of pro-inflammatory cytokine (IL-6) and lower expression of IGF-II in comparison to broiler chicken. These findings were as per expectation in view of much higher immuno-competence and lower growth rate in guinea fowl in comparison to chicken.
  Deepak Sharma and Suresh Kumar
  With the advancement in communication technology and the introduction of the data center networks, cloud computing etc has triggered the growth of internet data traffic volume at an exponentially high rate. This has increased the demand for networks that provides higher data rates and larger bandwidth. To accommodate this huge demand, next generation Orthogonal Frequency Division Multiplexing (OFDM) based optical networks are considered as a promising solution. In this study, we have presented an OFDM based optical network that can accommodate higher data rates and provides improved efficiency over traditional networks. The performance of the system is evaluated by measuring quality factor, Bit Error Rate (BER) and constellation diagrams using Optisys simulator.
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