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Articles by Deden Makbuloh
Total Records ( 1 ) for Deden Makbuloh
  Deden Makbuloh and Yessy Velina
  Diabetes mellitus is a metabolic disorder characterized by the presence of hyperglycemia due to defective insulin secretion, defective insulin action or both. One therapeutic approach for treating diabetes is to decrease the post-prandial hyperglycemia. This is done by preventing the absorption of glucose through the inhibition of the carbohydrate-hydrolysing enzymes α-glucosidase and α-amylase in the digestive tract. Actinomycetes have been known as source of commercialized acarbose, an α-glucosidase inhibitor. Acarbose is keto analog moieties of the C7N aminocyclitols. Sedoheptulose 7-phosphate is converted into 2-5 epi-valiolone via the activity of sedoheptulose 7-phosphate cyclase at the first step of the biosynthesis of C7N aminocyclitol. This research was aimed to detect and cloning sedoheptulose 7-phosphate cyclase gene. Detection gene was done by using Polymerase Chain Reaction (PCR) with with design primers C7N aminocyclitol. The primer used was designed on the basis of the comparison of the sequence of known sedoheptulose 7-phosphate cyclase (ACBC) from that of Actinoplanes sp. SE50/100 which was then cloned by T-Vector pMD20. The result show that there was similiarity of nucleotide series sedoheptulose 7-phosphate cyclase of Streptomyces sp. IPBCC. B. 15.1539 with GenBank database analysis through blast program. The result indicated that the designed primer was able to amplify the sedoheptulose 7-phosphate cyclase acarbose although the accomplishment in amplifying the gene is still up to 300 bp and have 100% sequence similiarity with DNA fragment of sedoheptulose 7-phosphate cyclase Actinoplanes sp. complete acarbose (ACB) gene cluster, strain SE50/110, accses number Y18523.4 reported in GenBank.
 
 
 
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