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Articles by David J. Harrison
Total Records ( 3 ) for David J. Harrison
  Renald Blundell and David J. Harrison
  The disruption of cell-matrix interactions influences the expression of a number of cell cycle regulatory proteins such as p21, p27 and p53. In this study, Clara cells obtained form both wildtype (wt) and p21 knockout (p21 ko) mice were isolated and cultured on different ECM combinations and the expression of three cyclin kinase inhibitors p21, p27 and p53 were studied. Distruption of the cell-matrix resulted in a decrease in the expression of cytoplasmic p21 and cytoplasmic p27. Nuclear p53 expression was found to increase in Clara cells lacking p21 and although the variation of ECM combinations did not affect the expression of p53. Although the p27 expression did not significantly changes upon culturing the cells in different compositions of ECM, disruption of the cell-matrix interactions seems to be influential.
  Renald Blundell and David J. Harrison
  Cell-matrix interactions or disruption affects the cell cycle regulation in various ways and thus the extracellular Matrix (ECM) can control cell differentiation. In this study, Clara cells obtained form both wildtype (wt) and p21 knockout (p21 ko) mice were isolated and cultured on different ECM combinations. A lower expression of cytokeratins 8, 18 and 19 in unattached Clara cells from p21 knockout (ko) mice compared to wildtype (wt) mice was observed. The expressions of cytokeratin 8 and 19 were significantly higher in primary Clara cells cultures when laminin formed part of the ECM composition. The cell-matrix disruption also played an important part on Clara cells differentiation process.
  Renald Blundell and David J. Harrison
  Extra Cellular Matrix (ECM) contains signals that control cell progression, division and death. Cell-matrix interactions or disruption affects the cell cycle regulation in various ways. In this study, Clara cells obtained form both wild type (wt) and p21 knockout (p21 ko) mice were isolated and cultured on different ECM combinations. Different ECM compositions did not influence Clara cells proliferation both the in presence or absence of p21. On the other hand, p21 was found to induce both apoptosis and necrosis in primary Clara cells cultures. The cell-matrix disruption rather than ECM composition seems to have a greater influence on the cell cycle regulation and progression.
 
 
 
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