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Articles by David G. Smith
Total Records ( 3 ) for David G. Smith
  Mushtaq Ahmad , David G. Smith and Shahid Mahboob
  The effect of NaCl on heat tolerance of Enterococcus faecium BAR1 and Enterococcus faecalis MI2 was determined at 55, 60 and 62.5oC for half an hour. Cells of both isolates were grown in Brain Heart Infusion broth containing 6.5% NaCl. Exponential phase cells were used as inoculants. Cells of both isolates were found resistant to heat treatment as compared with cells grown in BHI broth without presence of salt. The decimal reduction times (D-values) were determined by 1 log fall in viable count. Both isolates showed similar results at 55oC but at 60oC E. faecalis MI2 showed higher D-value than E. faecium BAR1. At 62.5oC E. Faecium BAR1 was found more tolerant to heat treatment than E. faecalis MI2. Experiments showed that the presence of salt in the growth medium induces more heat tolerance in enterococci than the cells grown in normal medium.
  Mushtaq Ahmad , David G. Smith and Shahid Mahboob
  Effect of pH of the growth medium on heat tolerance was investigated in Enterococcus faecium BAR1 and Enterococcus faecalis MI2. The heat tolerance was determined at 55, 60 and 62.5oC for half an hour. Cells of both isolates were grown to exponential phase at pH 5 or 7.4. Cells grown at 37oC at pH 5.0 were found tolerant to heat treatment as compared with cells grown at pH 7.4. Cells did not differ in their tolerance to heat at 55oC but at 60 and 62.5oC, cells of E. faecium BAR1 were found more heat tolerant than the cells of sE. faecalis MI2. The acidic pH of the growth medium had affected the susceptibility of E. faecium BAR1 and E. faecalis MI2 on heat tolerance. The cells grown at acidic pH showed greater heat tolerance that the cells grown at neutral pH.
  Mushtaq Ahmad , David G. Smith and S. Mahboob
  The heat resistance of exponential phase cells of environmental barley isolates Enterococcus faecium BAR 1 and a hospital isolate Enterococcus faecalis MI 2 grown at 37 or 45 was determined at 55, 60 and 62.5 ° C for 30 min. From the survival curves, the decimal reduction times (D-values) were determined. Cells grown at 45° C showed higher D-values. It was determined that the exponential phase cells grown at 45 were more resistant to heat than the heat resistance of cells grown at 37° C. The cells of both isolates were found resistant to heat treatment at 55, 60 and 62.5 as compared with the control in which cells were grown at 37° C and treated at 62.5° C.
 
 
 
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