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Articles by Dattadewi Purwantini
Total Records ( 6 ) for Dattadewi Purwantini
  Ismoyowati and Dattadewi Purwantini
  This research was aimed to evaluate genetic variation as well as genetic relationship among three breeds of local duck originated from Tegal, Magelang and Mojosari, Java, Indonesia. Six microsatellite markers were used in the analysis. All DNA marker profiles derived from six microsatellite primers showed polymorphisms with allele sizes ranged from 80-760 bp. The smallest allele was APH-24 (90-290 bp). Among all loci obtained from the whole population, ADL-231 showed the highest (0.828) and APH-23 showed the lowest (0.412) Polymorphic Information Content (PIC) values. The average PIC values of duck populations from Mojosari, Magelang and Tegal were 0.620, 0.699 and 0.76, respectively. There was a great variation in estimation of heterozygosity (He) values in various duck populations, i.e., 0,509, 0,695 and 0,728 each for duck populations from Mojosari, Tegal and Magelang. The Hardy-Weinberg Equilibrium (HWE) analysis showed deviations in all loci analyzed due to selection and migration occurred in the three populations. Genetic distance analysis indicated that the closest genetic relationship was found between Tegal and Magelang duck populations (0.1722) while the farthest one occurred between Tegal and Mojosari populations (0.3251). In conclusion, estimation of genetic diversity in duck population in Java using microsatellite marker in this research showed a great variation based on PIC and heterozygosity values. In addition genetic relationship between duck populations from Tegal and Magelang was closer compared to that between Tegal and Mojosari.
  Ismoyowati and Dattadewi Purwantini
  Identification and characterization of Indonesia local ducks are needed since the information is so important for the Indonesia germ plasm data bank as well as for assisting the genetic improvement program of the species. The identification can be based on qualitative or quantitative phenotype or based on biological molecular through DNA polymorphisms. The research aimed to evaluate the genetic diversity based on the phenotype and genetic relationship using 7 microsatellite primers between Bali and Alabio ducks of Bali and South Borneo region, respectively. A survey has been conducted on farmers of Mengwi (Denpasar, Bali) and Amuntai (Hulu Sungai Utara, South Borneo). Two farmers were chosen randomly on each region and 10 animals were sampled on each farmer making all 40 animals included in the study. The results showed that: the two local ducks differ phenotypically, Bali ducks produced less eggs than did the Alabio and only 5 microsatellite primers were polymorphic. The highest PIC value was of ADL-29 (0.588) with the mean PIC of the Bali and Alabio population of 0.446 and 0.5, respectively. The estimated heterozygosity value on that locus showed a big variation between the two local ducks, i.e. Bali ducks (0.683) and Alabio (0.813). The Hardy-Weinberg Equilibrium test showed that the Bali duck population was a result of random mating while the Alabio was not. The genetic distance evaluation showed that the Bali and Alabio ducks had a genetic relationship of 0.06. The study concluded that the genetic variability of the two local ducks based on phenotype and microsatellite marker was quite high. Bali ducks and Alabio ducks were found to be distantly genetically related.
  Dattadewi Purwantini , Tri Yuwanta , Tety Hartatik and Ismoyowati
  This research aimed to determine mitochondrial DNA (mtDNA) D-loop region polymorphism and phylogenetic in five Indonesian native ducks population namely Magelang duck, Tegal duck, Mojosari duck, Bali duck and Alabio duck. The significance of this research was applicable to conservation and refinement strategy as well as the improvement of genetic quality by utilizing the available native duck plasma nutfah. It further concerned with determining the genealogy of family, channel, breed or maternal inheritance that bred individuals in native duck population in Indonesia and discovering phylogenetic relations with Mallards duck (Anas platyrhynchos) and the other Anas ducks. Fragment of 718-bp was amplified by PCR and determined the nucleotide sequence of 701-bp. The sequence was then analyzed using Single Nucleotide Polymorphism (SNP) and compared to the standard nucleotide sequence from Anas Platyrhynchos complete genome (HM010684.1) in GenBank Accession. It obtained nucleotide percentage equation of 93.59±8.23%. Phylogenetic investigation used sequencing products and was analyzed using MEGA5 software. Indonesian native ducks have a relatively close genetic relationship with Anas Platyrhynchos and Anas zonorhyncha shown by the genetic distance varying from 0.000-0.786 compared to the other Anas ducks in the world (0.073-1.037) or to Cairina moschata (2.972-5.776). Highly distant genetic variation was found in Magelang duck compared to the other native ducks ranged from 0.000-0.950 to 0.000-0.312. The research concluded that polymorphism of mtDNA D-loop region was found in Indonesian native ducks and had relatively similar maternal inheritance with Anas platyrhyncos dan Anas zonorhyncha.
  Ayu Rahayu , Dattadewi Purwantini , Dyah Maharani and Tety Hartatik
  The objective of this study was to investigate the variations in Melanocortin 1 receptor (MC1R) gene and the association with plumage colours in Magelang duck populations using single nucleotide polymorphism (SNP). Forty three genomic DNA samples of Magelang ducks consisted of 3 plumage colour groups of brown, black and white were investigated. Primer design from Anas platyrhynchos complete genome in GenBank Accession Number HQ699486. Primer F:5’-GCTCTTCATGCTGCTGATGG-’3, R:5’-GATGAAGACGGTGCTGGAGA-’3. A 256-bp fragment was amplified by polymorphism chain reaction then sequenced. Two SNPs identified in this study were c.376A>G and c.409G>A. Chi-square (X2) value showed that 0.75 in Magelang ducks had GG genotype; 0.81 for GA genotype; 0.34 for AA genotype (c.376G>A) and 0 for GG genotype; 1.37 for GA genotype; 5.82 for AA genotype (c.409A>G). These results suggested that the MC1R genotype distribution in Magelang duck population with brown, black and white plumage was balanced or not deviated from Hardy Weinberg Equilibrium. Conclusively, two SNPs were identified; c.376A>G SNP that changed the amino acid from isoleucine to valine and valin/isoleucine and c.409G>A SNP that changed alanine to threonine and threonine/alanine.
  Dattadewi Purwantini , Setya Agus Santosa and Ismoyowati
  Background and Objective: The egg productivity of Indonesian local duck is higher than that of other local poultry. Follicle stimulating hormone (FSH) is part of the glycoprotein hormone that plays an important role in stimulating egg production by the ovaries. The FSH gene can be identified based on its nucleotide sequence by using the single nucleotide polymorphism (SNP) technique. The aim of this study was to identify SNPs of the FSH gene and investigate the SNP associations with egg production characteristics in two native Indonesian ducks, including the Tegal duck, the Magelang duck (F0) and reciprocal crosses named Gallang and Maggal (F1). Materials and Methods: This study used 200 ducks, comprising 50 of each breed. FSH gene amplification used the forward primer FSH-AnasPF: L 556 5’- TTCAGGCCTCCCCTACTTCT-3’ and reverse FSH-AnasPR: H 820 5’- GTGCTGCAAGGCTTTTTAGG-3’. The SNP was determined according to the BioEdit v7.2.0 program through the ClustalW menu (accessory application). Allele and genotype frequencies were applied to observe the Hardy-Weinberg (H-W) equilibrium. The SNP genotype gene FSH associated with egg production was subject to quantitative trait loci (QTL) analysis. Results: The research successfully amplified a 264-bp PCR product to identify SNPs. SNPs were found at 700-nt (c.700T>C) and 701-nt (c.701G>A) in the H-W equilibrium. The characteristics of egg production were affected by a double allele C (dominant) and A (recessive). Therefore 3 genotype pairs CC, CA and AA for all populations determined the high, medium or low egg production. The effects of the average gene C (α1) in the Tegal duck, Magelang duck and reciprocal crosses were 15.25; 13.74; 12.23 and 11.01, respectively, whereas gene A (α2) had a negative effect and led to values of -12.99 -14.30, -11.29 and -11.46, respectively. Conclusion: SNP genotypes of the FSH gene in Tegal, Magelang and the reciprocal cross ducks were polymorphic and associated with egg production characteristics. SNP genotypes of the FSH gene (SNP c.700T>C and SNP c.701G>A) were viable candidates for marker assisted selection (MAS) to determine the egg productivity of Indonesian local ducks.
  Ismoyowati , Agus Susanto , Dattadewi Purwantini , Elly Tugiyanti and Aziz Noor Awalludin
  Background and Objective: The body shape or morphological characteristics of Muscovy ducks are determined by the length of the femur, tibia, tarsometatarsus, tarsometatarsus circumference, 3rd-digit, wings and maxilla. Identification of the feather color of Muscovy ducks is important because feather color determines the physical quality of the carcass and affects the level of consumer preference. The aim of this research was to assess the genetic variation of native Indonesian Muscovy ducks based on morphometric traits and gene sequence variation of Melanocortin 1 receptor (MC1R) and its genotypic association with different feather colors. Methodology: Two hundred day-old Muscovy ducks consisting of the white and white-black feather color combination in the same proportion between male and female ducks were included in the study. Differences in body weight and morphometric measurements among the groups were evaluated by ANOVA with Systat version 13. Primer design used Clustal X, based on Cairina moschata MC1R gene, partial cds (KX013541.1) from the GenBank database, the primary forward sequence: 5’-GCTCTTCATGCTGCTGATGG-’3 and reverse primer: 5’-GATGAAGACGGTGCTGGAGA-’3. Results: The male Muscovy ducks have larger morphometric features than females. Male Muscovy ducks with the white-black feather color combination had the heaviest body weight. A general linear F-test analysis separately showed that only the neck length, length of third digit and sex significantly affected the animals' body weight. The identification of feather color of Muscovy ducks showed that there are variations within a group of white-black feather color combination of Muscovy ducks. Feather color variation was observed on the head, wings, breast, tail and plumage. The sequencing of PCR products resulted in nucleotide polymorphism. The GG genotype was observed in 293 nt in the white-black population and the CC genotype was observed in white-black and white feather colors in both male and female Muscovy ducks. Conclusion: Muscovy ducks with the white-black feather color combination had heavier body weights than those with the white feather color. The neck length can be used to predict the body weight of Muscovy ducks. Muscovy ducks had a variety of feather colors ranging from white to the white-black color combination. The MC1R gene polymorphism was observed in Muscovy ducks. Muscovy ducks with the white-black feather color grow faster and their live weight can be estimated by neck length.
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