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Articles by Dan Li
Total Records ( 13 ) for Dan Li
  Dan Li , Mengrou Ren , Jing Lu , Tong Wang , Yixuan Huang , Wei Jiang , Zhang Yan , Yuwen Fang , Xuming Deng and Shuang Guan
  The optimum enrichment parameters of main omega-3 polyunsaturated fatty acids (ω-3 PUFAs) from Rana egg oil were investigated. Firstly, it was optimized the saponification condition. Secondly, the PUFAs fractions from the first step were subjected to a urea complexation process using one-factor-at-a-time approach. Then, the enrichment time, temperature and urea to fatty acid ratio were mainly analyzed by response surface methodology. Consequently, it was gained the maximized relative contents of docosahexaenoic acid (DHA, 8.81%), docosapentenoic acid (DPA, 3.01%) and eicosapentaenoic acid (EPA, 8.31%) at an enrichment time of 16 h, an enrichment temperature of 0°C and a urea to fatty acid ratio of 4.5:1 (wt/wt). In addition, it was investigated the effect of enriched ω-3 PUFAs on cell viability and gained the inhibitory effect at dose of 2000 μg mL–1. This data suggested that Rana egg oil could be utilized as alternative source of ω-3 PUFAs.
  Chengming Wang , Frederik W. van Ginkel , Teayoun Kim , Dan Li , Yihang Li , John C. Dennis and Bernhard Kaltenboeck
  Severe chlamydial disease typically occurs after previous infections and results from a hypersensitivity response that is also required for chlamydial elimination. Here, we quantitatively dissected the immune and disease responses to repeated Chlamydia pneumoniae lung infection by multivariate modeling with four dichotomous effects: mouse strain (A/J or C57BL/6), dietary protein content (14% protein and 0.3% L-cysteine-0.9% L-arginine, or 24% protein and 0.5% L-cysteine-2.0% L-arginine), dietary antioxidant content (90 IU α-tocopherol/kg body weight versus 450 IU α-tocopherol/kg and 0.1% g L-ascorbate), and time course (3 or 10 days postinfection). Following intranasal C. pneumoniae challenge, C57BL/6 mice on a low-protein/low-antioxidant diet, but not C57BL/6 mice on other diets or A/J mice, exhibited profoundly suppressed early lung inflammatory and pan-T-cell (CD3δ+) and helper T-cell (CD45) responses on day 3 but later strongly exacerbated disease on day 10. Contrast analyses characterized severe C. pneumoniae disease as being a delayed-type hypersensitivity (DTH) response with increased lung macrophage and Th1 cell marker transcripts, increased Th1:Th2 ratios, and Th1 cytokine-driven inflammation. Results from functional analyses by DTH, enzyme-linked immunospot, and immunohistofluorescence assays were consistent with the results obtained by transcript analysis. Thus, chlamydial disease after secondary infection is a temporal dysregulation of the T-cell response characterized by a profoundly delayed T-helper cell response that results in a failure to eliminate the pathogen and provokes later pathological Th1 inflammation. This delayed T-cell response is under host genetic control and nutritional influence. The mechanism that temporally and quantitatively regulates the host T-cell population is the critical determinant in chlamydial pathogenesis.
  Guang Zhu , Hai-bo Wang , Feng Jin , Li-de Tao , Dan Li , Teng-yang Ni , Wen-yuan Li , Bo Pan , Wei-ming Xiao , Yan-bing Ding , Masataka Sunagawa and Yan-qing Liu
  Background and Objective: The traditional Chinese medicine herb Celastrus Orbiculatus is a folk medicinal plant that has been used in various diseases. The ethyl acetate extract of Celastrus orbiculatus (COE) was reported to show significant anti-tumor effects. Prohibitin (PHB), an evolutionarily-conserved protein has been found to be over-expressed in Gastric Cancer (GC) and be closely related with tumor malignancy. In this study the effects of COE and small interfering RNA (siRNA)-mediated silencing of PHB gene expression on the migration and invasion of GC was investigated. Materials and Methods: The effects of COE on the expression of PHB in MGC-803 GC cells were evaluated by Western Blots. Small interfering RNA (siRNA) sequences targeting PHB were transfected into the MGC-803 cell line. The effect of COE on PHB silenced GC cell viability was examined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of COE and siRNA-mediated silencing of PHB gene expression on invasion and migration of GC and protein expression of tumor cells were analyzed by wound-healing assay, trans well assay and Western Blots, respectively. Results: It was found that COE reduced PHB expression in MGC-803 cells. In addition, invasion and migration of GC was significantly decreased upon PHB silencing. Gene silencing of PHB increased E-cadherin expression, decreased N-cadherin, Vimentin expression and inhibited c-Raf/ERK signaling pathway. Moreover, COE effectively inhibited GC invasion and migration, EMT and c-Raf/ERK signaling pathway of PHB silenced MGC-803 cells in a dose-dependent manner. Conclusion: The COE inhibits migration and invasion probably via suppressing PHB expression, which correlates with c-Raf/ERK signal pathway inhibition in MGC-803 cells.
  Dan Li , Feng Jin , Tengyang Ni , Li Tao , Haibo Wang , Qingtong Shi , Yayun Qian , Yanqing Liu and Yali Diao
  Background and Objective: Human Esophageal Squamous Cell Carcinoma (ESCC) is one of the most common malignancies and the most frequent reason of cancer death around the world. Jinlong Capsule (JLC) is a Traditional Chinese Medicine (TCM) and has been used for several decades to treat cancers with significantly improved clinical outcomes as adjuvant therapy. In the present study, the effect of JLC on anti-proliferative, pro-apoptotic activities of human Eca-109 and EC9706 cells in vitro were investigated. Materials and Methods: The MTT assay was used to detect cell viability. Apoptosis analysis by flow cytometry was used to detect the percentages of the living cells as well as early, late apoptotic cells and necrotic cells. Ultrastructure observation using Transmission Electron Microscopy (TEM) was used to observe the ultrathin sections. Mitochondrial Transmembrane Potential (MTP) assessment, immunofluorescence staining and expression level of proteins by western blotting were detected its mechanism. Results: All the results showed that JLC could decrease proliferation and induce apoptosis in ESCC cells. Moreover, JLC might increase the expression of pro-apoptotic proteins caspase-8 and caspase-9 by activating the extrinsic and the intrinsic apoptotic pathways. The anti-apoptosis protein B-cell lymphoma-2 (Bcl-2) also could promote mitochondrial-mediated apoptosis by inducing Mitochondrial Outer Membrane Permeabilization (MOMP) in the study. Besides, the MAPK signal pathway took function in ESCC cells that treated with different concentrations of JLC. Conclusion: These findings suggested that JLC may be a promising agent worthy of being investigated for the treatment of human ESCC in vitro. The JLC holds the multi-targeted therapeutic effects of TCM.
  Guang-Hua Xie , Wei Xie , Heyun Piao and Dan Li
  Background and Objective: Cyanidin-3-glucoside (C3G) is one of the major bioactive compounds in anthocyanins, which have been shown to prevent a large spectrum of diseases. It has been reported that hypoxia is a common characteristic of many types of solid tumor and that the hypoxia-inducible factor 1α (HIF-1α) plays an important role in the process of angiogenesis and metastasis in cancer cells. The objective of this study was to explore the effects of C3G on HIF-1α activation in cholangiocarcinoma (CCA) cells. Materials and Methods: The human cholangiocarcinoma cell line QBC939 was used in this study. Hypoxic conditions were achieved by using the chemical hypoxia agent cobalt chloride or incubation at 1% O2. and MTT assay, Luciferase reporter assay, flow cytometric apoptosis assay, western blot, RT-PCR and immuno fluorescence methods were used to determine the C3G efficacy. Results: C3G markedly decreased the hypoxia-induced accumulation of HIF-1α reporter activity and protein expression in a dose-dependent manner and exerted a large apoptotic effect in CCA cells. Furthermore, C3G reduced expression of several genes downstream of HIF-1α, such as vascular endothelial growth factor and erythropoietin, without affecting the expression level of HIF-1α mRNA, thus indicating that C3G acts as an inhibitor of HIF-1α mRNA translation. Conclusion: This study provides a novel strategy to develop a low toxicity, high efficiency and multi-target natural active substance for the treatment of cancers.
  Wei Chen , Jinghua Ma , Hui Zhang , Dan Li and Xianfu Zhang
  The Red-crowned crane (Grus japonensis) is one of the largest birds in East Asia which is among the rarest and endangered cranes in the world. In order to enhance population management and further conservation of Red-crowned cranes, researchers compared behavioural changes from wild population to captive population. Meanwhile, researchers also compared the artificial inbred population with the natural normal populations. In this research, five main behavioural patterns of Red-crowned cranes include resting, moving, preening, feeding and alerting were identified by all occurrence sampling and instantaneous scanning sampling methods with 5-10 min intervals. The referred wild and captive populations were concluded from the previous publications and the artificial inbred populations were observed in Hangzhou Wildlife Park (China) during August and September, 2009. As a result, researchers found the difference (p>0.05) between wild and captive populations is not significant. However, preening in captive population is higher than in wild population. The wild population spent more time for resting compared to captive population yet feeding is the most time-spent behaviours for both populations. Furthermore, behavioural patterns between normal and inbred populations are found significantly different (p<0.05). Alerting and resting behaviours are significantly higher in the normal population than in inbred population. Moreover, moving is the dominant behaviour of inbred population but feeding is taken the most time by normal population.
  Dan Li , Jiaqi Wang , Fadi Li and Dengpan Bu
  Methanogens and protozoa in the rumen negatively affect rumen function by wasting ingested energy. It is desirable to modulate rumen fermentation by cost-effective dietary intervention. The objective of this study was to evaluate effects of unsaturated C18 fatty acids (oleic, linoleic and linolenic acids), either alone or in combination with malic acid on in vitro ruminal fermentation, protozoa and Methanobacterium formicicum. Rumen fluid collected from ruminally fisulated lactating Chinese Holstein cows served as the inoculum and the diet consisted of alfalfa hay and corn (50:50). The results showed addition of unsaturated fatty acids tended to increase fermentation pH (p>0.05) and degree of unsaturation of fatty acids tended to affect such effect on the pH (p>0.05). Acetate, butyrate, total VFA, total gas production and methane production decreased (p<0.01) with increasing degree of unsaturation of C18 fatty acids but addition of malic acid did not have any additive or synergistic effect except for propionate which was decreased by the addition of malic acid (p<0.01). Both malate and the unsaturated fatty acids, either alone or in combination, decreased methane production (p<0.01) with combination of these two types of acid further decreasing methane production. Except for oleici acid that decreased population of M. formicicum decreased (p<0.01), either protozoa or M. formicicum was affected by these fatty acid and malic acid. It was concluded that when used together, malic acid and fatty acids could reduce methane emission without negative impairing fermentation in vitro.
  Lu Feng , Bin Liu , Yanqun Liu , Yuli A. Ratiner , Bo Hu , Dan Li , Xiaolin Zong , Wei Xiong and Lei Wang
  The occurrence of unilateral flagellar phase variation was previously demonstrated in Escherichia coli strains carrying the non-fliC flagellin-specifying locus flk. In this study, we investigated the mechanism involved in this process. By using sequencing and sequence analysis, the flk region between the chromosomal genes yhaC and rnpB was characterized in all described flk-positive E. coli strains, including the H35 strain identified in this study (the other strains used are H3, H36, H47, and H53 strains), and this region was found to contain a putative integrase gene and flanking direct repeats in addition to the flk flagellin-specifying gene flkA and a fliC repressor gene, flkB, indicating that there is a typical genomic islet (GI), which was designated the flk GI. The horizontal transfer potential of the flk GI was indicated by detection of the excised extrachromosomal circular form of the flk GI. By generating fliC-expressing variants of H3 and H47 strains, unilateral flagellar phase variation in flk-positive strains was shown to be mediated by excision of the flk GI. The function of the proposed integrase gene was confirmed by deletion and a complementation test. The potential integration sites of the flk GI were identified. A general model for flagellar phase variation in flk-positive E. coli strains can be expressed as fliCoff + flkAonfliCon + flkAnone. This is the first time that a molecular mechanism for flagellar phase variation has been reported for E. coli.
  Jun Wang , Dan Li , Jingqun Gao , Bin Liu , Baoxin Wang , Dan Wang , Tingting Fan and Xiangdong Zhang
  Two complexes, (enH2)3[TbIII(ttha)]2 · 11H2O (1) (en = ethylenediamine and H6ttha = triethylenetetramine-N, N, N ', N '', N ''', N '''-hexaacetic acid) and (enH2)[TbIII(pdta)(H2O)]2 · 8H2O (2) (H4pdta = propylenediamine-N, N, N ', N '-tetraacetic acid), were synthesized and characterized by elemental analysis, infrared spectrum, UV-Vis spectrum, fluorescence spectrum, and single-crystal X-ray diffraction. The central TbIII of 1 is nine-coordinate, pseudo-monocapped square antiprism with four nitrogens and five oxygens from one ttha, and crystallizing in the monoclinic crystal system with P21/n space group. There is a free (non-coordinate) carboxylate (-CH2COO-) in the [TbIII(ttha)]3-. The central TbIII of 2 is eight-coordinate in a standard square antiprism with two nitrogens and four oxygens of one pdta, one oxygen from a carboxylate of an adjacent pdta, and one oxygen from water, crystallizing in the monoclinic crystal system with C2/c space group. Binding between the enH22+ with [TbIII(ttha)]3- or [TbIII(pdta)(H2O)]- is reviewed, providing the basis for interaction of TbIII complexes with biomolecules.
  Jingqun Gao , Dan Li , Jun Wang , Xudong Jin , Pingli Kang , Tong Wu , Kai Li and Xiangdong Zhang
  A 2-D binuclear coordination polymer, {[YIII(Hpdta)(H2O)]2 ·6H2O} n (H4pdta = propylenediamine-N,N,N′,N′-tetraacetic acid), has been synthesized through direct hydrothermal reaction and characterized by infrared spectrum and thermal analysis. Single-crystal X-ray diffraction reveals that Y(III) is eight-coordinate of almost standard square antiprismatic polyhedron.
  Jingqun Gao , Dan Li , Jun Wang , Xudong Jin , Tong Wu , Kai Li , Pingli Kang and Xiangdong zhang
  Two lanthanide complexes, (enH2)[SmIII(egta)(H2O)]2 • 6H2O (1) (en = ethylenediamine and H4egta = ethyleneglycol−bis−(2−aminoethylether)−N,N,N′,N′−tetraacetic acid) and (enH2)1.5[SmIII(ttha)] • 4.5H2O (2) (H6ttha = triethylenetetramine−N,N,N′,N″,N′″,N′″−hexaacetic acid), have been synthesized and characterized by IR spectroscopy, thermal analysis, and single-crystal X−ray diffraction. The (enH2)[SmIII(egta)(H2O)]2 • 6H2O is mononuclear nine−coordinate and crystallizes in the monoclinic crystal space group P21/n with the cell dimensions a = 13.0563(13) Å, b = 12.6895(11) Å, c = 14.9497(15) Å, β = 105.782(2)°. The polymeric (enH2)1.5[SmIII(ttha)] • 4.5H2O is also nine−coordinate crystallizing in the monoclinic crystal space group P21/n, but with cell dimensions a = 17.7800(16) Å, b = 9.7035(10) Å, c = 22.096(2) Å, β = 118.8740(10)°. Each ethylenediammonium (en ) cation in (enH2)[SmIII(egta)(H2O)]2 • 6H2O connects three adjacent [SmIII(egta)(H2O)] anions through hydrogen bonds, while in (enH2)1.5[SmIII(ttha)] • 4.5H2O, there are two types of en cations, which form hydrogen bonds with the neighboring [SmIII(ttha)]3− anions, leading to the formation of a 2−D ladder-like layer structure.
  Chongyang Wang , Dan Li , Danni Wang and Shuisen Chen
  Background and Objective: Monitoring the spatiotemporal variation of suspended sediment (SSC) concentrations in estuaries and coasts can be helpful to improving the understanding of material cycle and environmental change. This study analyzed the temporal and spatial changes of suspended sediment concentrations in Hanjinag River Estuary. Materials and Methods: Based on the suspended sediment concentrations retrieved from 50 scenes of Landsat imageries during 1988-2015, the temporal and spatial changes of suspended sediment concentration in high flow season and low flow seasons in the regions within different distance from the coastline of Hanjiang River Estuary were analyzed and compared. Results: It was found that the result of suspended sediment concentration retrieved from Landsat imageries has a high accuracy compared to the record of Chao’an hydrological station in lower reaches of Hanjiang River. The suspended sediment concentrations in Hanjiang River Estuary showed a significant spatial distribution, with a spatial decreased trend from the outlets of Hanjiang River to the South China Sea, at a change rate of about 7.56 mg L–1 km–1. From 1988-2015, there are decreased trend change of suspended sediment concentrations in both high and low flow seasons in Hanjiang River Estuary but no period change. The annual average suspended sediment concentration decreased by about 7 and 6.8 mg L–1 in high and low flow season in Hanjiang River Estuary from 1988-2015, respectively. Besides, it was also found that suspended sediment concentrations in low flow season in Hanjiang River Estuary were 56 mg L–1 higher than that of high flow season, which deserves the further study. Conclusion: Evidently, retrieving suspended sediment concentrations from a long time series of remote sensing data is very helpful to understand the temporal and spatial change of suspended sediment concentrations in estuaries, coasts and P to increase awareness of environmental changes, material cycle and human activity effects.
  Roberto Carreno , Dan Li , Mehmet Sen , Iris Nira , Tatsuo Yamakawa , Qing Ma and Glen B. Legge
  MEM83 is an inserted domain (I-domain)-specific antibody that up-regulates the interaction of LFA-1 with ICAM-1 through an outside-in activation mechanism. We demonstrate here that there is no change in the affinity of the MEM83 antibody for the I-domain in either its low (wild-type) or high affinity form and that MEM83 does not enhance the binding of the wild-type I-domain to ICAM-1. Furthermore, we show that the antibody acts as an activating agent to induce LFA-1/ICAM-1-dependent homotypic cell aggregation only as an IgG, but not as a Fab fragment. On the basis of these data, we propose an avidity-based mechanism that requires no direct activation of the LFA-1 I-domain by the binding of the antibody; rather, activation is enhanced when there is an interaction with both arms of the IgG. A molecular model of the antibody interaction with LFA-1 illustrates the symmetry and accessibility of the two MEM83 epitopes across the LFA-1/ICAM-1 heterotetramer. We hypothesize that MEM83 stabilizes adjacent LFA-1 molecules in their active form by the free energy that is gained from the binding of the I-domains to each arm of the IgG. This leads to stabilization of the open state of the integrin and outside-in signaling. Our model supports a mechanism in which both affinity and avidity regulation are required in the activation of LFA-1.
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