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Articles by Dan Huang
Total Records ( 3 ) for Dan Huang
  Dan Huang , Yun Shen , Liyou Qiu , Crystal Y. Chen , Ling Shen , Jim Estep , Robert Hunt , Daphne Vasconcelos , George Du , Pyone Aye , Andrew A. Lackner , Michelle H. Larsen , William R. Jacobs Jr. , Barton F. Haynes , Norman L. Letvin and Zheng W. Chen
  Little is known about the immune distribution and localization of antigen-specific T cells in mucosal interfaces of tissues/organs during infection of humans. In this study, we made use of a macaque model of Mycobacterium tuberculosis infection to assess phosphoantigen-specific Vγ2Vδ2 T cells regarding their tissue distribution, anatomical localization, and correlation with the presence or absence of tuberculosis (TB) lesions in lymphoid and nonlymphoid organs/tissues in the progression of severe pulmonary TB. Progression of pulmonary M. tuberculosis infection generated diverse distribution patterns of Vγ2Vδ2 T cells, with remarkable accumulation of these cells in lungs, bronchial lymph nodes, spleens, and remote nonlymphoid organs but not in blood. Increased numbers of Vγ2Vδ2 T cells in tissues were associated with M. tuberculosis infection but were independent of the severity of TB lesions. In lungs with apparent TB lesions, Vγ2Vδ2 T cells were present within TB granulomas. In extrathoracic organs, Vγ2Vδ2 T cells were localized in the interstitial compartment of nonlymphoid tissues, and the interstitial localization was present despite the absence of detectable TB lesions. Finally, Vγ2Vδ2 T cells accumulated in tissues appeared to possess cytokine production function, since granzyme B was detectable in the γδ T cells present within granulomas. Thus, clonally expanded Vγ2Vδ2 T cells appeared to undergo trans-endothelial migration, interstitial localization, and granuloma infiltration as immune responses to M. tuberculosis infection.
  Lin Yan , Jin Qiu , Jianbo Chen , Bridgett Ryan-Payseur , Dan Huang , Yunqi Wang , Lijun Rong , Jody A. Melton-Witt , Nancy E. Freitag and Zheng W. Chen
  While recombinant Listeria monocytogenes strains can be explored as vaccine candidates, it is important to develop attenuated but highly immunogenic L. monocytogenes vaccine vectors. Here, prfA* mutations selected on the basis of upregulated expression of L. monocytogenes PrfA-dependent genes and proteins were assessed to determine their abilities to augment expression of foreign immunogens in recombinant L. monocytogenes vectors and therefore enhance vaccine-elicited immune responses (a prfA* mutation is a mutation that results in constitutive overexpression of PrfA and PrfA-dependent virulence genes; the asterisk distinguishes the mutation from inactivation or stop mutations). A total of 63 recombinant L. monocytogenes vaccine vectors expressing seven individual viral or bacterial immunogens each in nine different L. monocytogenes strains carrying wild-type prfA or having prfA* mutations were constructed and investigated. Mutations selected on the basis of increased PrfA activation in recombinant L. monocytogenes prfA* vaccine vectors augmented expression of seven individual protein immunogens remarkably. Consistently, prime and boost vaccination studies with mice indicated that the prfA(G155S) mutation in recombinant L. monocytogenes ΔactA prfA* strains enhanced vaccine-elicited cellular immune responses. Surprisingly, the prfA(G155S) mutation was found to enhance vaccine-elicited humoral immune responses as well. The highly immunogenic recombinant L. monocytogenes ΔactA prfA* vaccine strains were as attenuated as the recombinant parent L. monocytogenes ΔactA vaccine vector. Thus, recombinant attenuated L. monocytogenes ΔactA prfA* vaccine vectors potentially are better antimicrobial and anticancer vaccines.
  Ling Zhao , Kaiyu Wang , Xun Wang , Dan Huang , Juchun Lin , Defang Chen , Gang Ye and Cheng Lv
  In this study, Response Surface Methodology (RSM) was employed to optimize the extraction conditions (extraction temperature, ratio of water to raw material and extraction time) of polysaccharides from the fruiting bodies of Chinese truffle Tuber sinense. A Box-Behnken design was used for experimental design and analysis of the results to obtain the optimal processing parameters. The optimal conditions were extraction temperature 94.75°C, ratio of water to raw material 16.41:1, extraction time 2.67 h. According to analysis, extraction temperature was the most significant factor to affect the yield of polysaccharides from Chinese truffle Tuber sinense. Pharmacological experiments indicated that CTP had potent immunomodulatory effects in immunosuppression mice model. At the dose of 50, 100 and 200 mg kg-1 body weight, a significant increase (p<0.01) in phagocytosis of macrophage was observed.
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