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Articles by Daljit Singh Arora
Total Records ( 2 ) for Daljit Singh Arora
  Priyanka Chandra and Daljit Singh Arora
  Background: Agricultural waste bioconversion aimed at producing fungal biomass is a highly attractive alternative because, besides resulting in products of commercial interest, it reduces the amount of waste thereby minimizing pollution. Materials and Methods: The present study was planned to investigate the antioxidant potential of fungi isolated from soil of different areas of Punjab, India. Screening of the fungal isolates for antioxidant activity was carried out by dot blot assay. Out of 120 fungal isolates, 51 of fungal isolates demonstrated antioxidant potential and 8 fungal strains with highest activity were further assayed quantitatively on different agro-residues (Wheat Straw (WS), Rice Straw (RS), Corn Cob (CC), Pea Pod (PP) and sugarcane baggases (SC)) by various assay procedures (DPPH assay, reducing power, ferrous ion and Nitric Oxide (NO) ion scavenging activity, ferric ion reducing antioxidant power (FRAP) assay). Total phenolic content was also estimated using Folins-Ciocalteau (FC) reagent. Results: All the eight fungal strains (Aspergillus fumigatus, Aspergillus terreus 1, Aspergillus terreus 2, Aspergillus wentii 1, Aspergillus wentii 2, Penicillium citrinum, Penicillium granulatum and Penicillium expansum) demonstrated good antioxidant activity assayed through various assay procedures and total phenolic content. All the agro-residues supported good antioxidant activity. Sugarcane baggases (SC) was the best substrate followed by Pea Pod (PP) for antioxidant activity. Conclusion: To the best of knowledge apparently this is the first systematic report on antioxidant activity of selected fungi. Agro-industrial residues can be reused for the production of different bioactive phenolic compound.
  Priyanka Chandra and Daljit Singh Arora
  Background: Fungi are the good source of various biological active secondary metabolites. Materials and Methods: The antioxidant potential of Penicillium expansum isolated from soil of Punjab, India was studied and a three-step optimization strategy which includes, one-factor-at-a-time classical method and different statistical approaches (Plackett-Burman design and response surface methodology) were applied to enhance the antioxidant potential. Antioxidant activity was assayed by different procedures and compared with total phenolic content. Results: Primarily, different carbon and nitrogen sources were screened by classical methods, which revealed sucrose as carbon source is most suitable for antioxidant activity. Sodium nitrate, yeast extract and peptone were good sources of nitrogen but sodium nitrate was the best among them. Significance of the components of Czapek dox’s medium with respect to antioxidant activity was evaluated with Plackett-Burman design, which supported sucrose and NaNO3 to be the most significant. In second step, sucrose and NaNO3 along with temperature were taken as three variables for response surface methodology to study their interaction. Response surface analysis showed significant enhancement in the antioxidant potential of Penicillium expansum. A compound was purified from the ethyl acetate extract which demonstrated potent antioxidant activity. Conclusion: The present study demonstrated potential of soil fungi to have antioxidant activity similar to plants and mushrooms thus further highlighting their significance as new sources of natural antioxidants and thus endorse the future prospects for the commercial production of natural and safer antioxidant compounds from such fungi. The fungi may provide easier set up for production and purification of natural antioxidants as compared to higher plants.
 
 
 
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