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Articles by Dahelmi
Total Records ( 5 ) for Dahelmi
  Wince Hendri , Djong Hon Tjong , Dahelmi and Dewi Imelda Roesma
  Background and Objective: Limnonectes blythii population in west Sumatra sharply decline because of its trading activity and damaged habitat. Conservation effort is necessary to protect the species. One of important information that needs to be done is to understand the species genetic variation. The aim of this study was to analyze the genetic variety of Limnonectes blythii of west Sumatra using Random Amplified Polymorphic DNA (RAPD). Materials and Methods: Thirty frogs were collected from three location of wildlife sanctuary in west Sumatra. Genetic variation analysis using RAPD with 11 OPA primer. The electrophoresis results were marked as 1 if a band founded and 0 if there’s no band. Results: Genetic variation of three population were low and the highest founded on Malampah population , next on Sijunjung and lastly Harau . However, genetic differentiation inter-population was considered moderate, which of half of overall genetic variation generated from differentiation inter-population and also a half from differentiation of intra-population which supported by lower heterozygosity inter-population than intra-population heterozygosity. Gen flow value of inter-population was observed moderate. Conclusion: Genetic variation of L. blythii intra-population is lower than inter-population and the gene flow between L. blythii population considered moderate.
  Dahelmi , Evy Sriganti and Suwarno
  Background and Objective: Larvae of the genus Cethosia is known associate with plants of the family Passifloraceae. The aim of this study was to obtain preliminary data on the life cycle of Cethosia hypsea that reared on Adenia macrophylla. Methodology: The immature stages (egg, larvae and pupae) were reared in the laboratory condition at temperature ranging between 26-30°C and relative humidity of 65-75%. Results: The incubation period of egg was 6-7 days. There are five instars stage of larva, the duration of instar 1st, 2nd, 3rd, 4th and 5th are 3, 3, 3, 3 and 4 days, respectively. Total larval phase is about 16 days, pupae formed after 1 day of the pre pupa period. Pupae period is about 7 days. Conclusion: The larvae of C. hypsea passed through five instars. The total development time from egg to adult eclosion ranged between 30-31 days. The life cycle duration of C. hypsea was about 34-35 days. This is the first study describing life cycle of Chetosia in Indonesia and this species capable of producing multiple generations per year.
  Emantis Rosa , Dahelmi , Siti Salmah and Syamsuardi
  Research of fluctuations in Diptera’s larvae in Phytotelmata had been conducted at three locations in West Sumatra, Indonesia; Padang, Bukittinggi and Payakumbuh, which aimed to determine the number and fluctuations Diptera larvae in Phytotelmata. The results obtained; the highest number of individual larvae Diptera in Phytotelmata was 7109 Aedes albopictus larvae (49.56%), followed by larvae of Culex tritaeniorhynchus with 2409 individuals (16.80%). Larvae fluctuated every month and tent to increase in November and December. There was no difference in the number of Diptera larvae individuals inhabiting pandan, taro, and pineapple, but there were significant differences between the three types of Phytotelmata (pandanus, taro and pineapple) with bamboo (p<0.05). Number of individual larvae in Phytotelmata negatively correlated with temperature and rainfall, but positively correlated with humidity (r = 0.44: p<0.05).
  Irham Falahudin , Siti Salmah , Dahelmi and Ahsol Hasyim
  A weaver ant (Oecophylla) is one of insects which is classified into Hymenoptera order and Formicidae family. There are two species of weaver ant, i.e., O. smaragdina which is spreaded along India, South East Asia till Australia and O. longinoda, which is distributed through Africa. Every ant’s nest has each own size which is different one to another. This nest’s size distinction causes the difference in the population dynamics of each colony as well. A weaver ant is a social insect that forms a kind of group named colony. A colony of Oecophylla consists of reproductive and non reproductive class. This research aims at observing the dynamics of weaver ant colonization toward the economics value and palm ecology. The method used in this research was a survey. Meanwhile, the samples were drawn by using hand collecting technique. The results of the research showed that there were 8 colonies of weaver ants which have varied sizes. The biggest size of the mosaic nest of weaver ants (Oecophylla smaragdina) gained in this study was (69:14, 2 cm) and the smallest one was (13:11 cm). Each mosaic has 2 spaces in average. The dynamics of a weaver ant colony are also varied. The biggest number of populations of ants in one nest found in this research were 7508 ind/nest and the smallest one was 578 ind/nest. The more ants found in the nest the higher the economics value.
  Hasmiwati , Jamsari , Yanwirasti , Nuzulia Irawati and Dahelmi
  Aedes aegypti is the major vector of DHF virus in the tropical and subtropical. The DHF prevention depends on vector control because the vaccine is still in development. Microsatellite has become on effective marker to obtain information about genetic diversity and analyze the structure of genetic population. The aim of this study was to isolate and characterize A. aegypti microsatellite markers, the Dengue Hemorrhagic Fever (DHF) vector in West Sumatra. Sequences containing microsatellites were obtained by enrichment method. Stages of works were as follows: isolation of A. aegypti genomic DNA, restriction with enzymes, ligation with adapters and hybridization by using microsatellite motifs. Furthermore, the candidate fragment contained motifs cloned on plasmid pGEM T easy vector using E. coli DH 5α with blue-white colony screening. The results showed 46 clones were successfully extracted from a total of 152 clones and became microsatellite motifs with repetition: (GA)3, (CTA)3, (GA)3 (TAAG)3, (ACTT)3 (TC)3 (AC)3. Eight pairs of primer were successfully designed from sequences containing microsatellite motifs with feasible flanking regions. The primer evaluation used 32 DNA samples of A. aegypti from 8 cities (population) in West Sumatra. These markers have been successfully amplified 9-17 alleles with amplification products ranging from 129-306 bp, with a high degree of polymorphism. Aedes aegypti microsatellite markers obtained can be used to analyze the structure of genetic population of A. aegypti and the obtained results were the additional microsatellite markers type of A. aegypti than what had previously existed.
 
 
 
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