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Articles by Da-Qian He
Total Records ( 3 ) for Da-Qian He
  Hua-Li Wu , Hui-Ying Wang , Da-Qian He , Yi Liu , Shao-Ming Gong , Fan-Li Kong and Qing Zhu
  Peroxisome Proliferator-Activated Receptor γ (PPAR-γ) was a key regulator of proliferation and preadipocyte differentiation in mammals. The objective of this study was to investigate the effect of the PPAR-γ gene on slaughter traits of 170 individuals from Zhejiang White geese (ZW) and the ontogenetic expression pattern. PCR-SSCP technique was developed to analyze coding region of the PPAR-γ gene polymorphism. The results showed that two genotypes (AA and AG) which were the products of two alleles (A and G) were observed. Association analysis indicated that this SNP was significantly associated with percentage of goose leg muscle (p<0.05) and the allele A was the predominant allele. Quantitative real-time PCR (qRT-PCR) assay measured the PPAR-γ mRNA expression in ZW and Landes goose five tissues of different ages (P1, P14, P21, P28, P35, P42, P49, P56 and P63). The results showed that the PPAR-γ mRNA had the highest expression level in goose abdominal fat and subcutaneous fat followed by lung and intestine. The PPAR-γ mRNA levels exhibited a rise-decline change in fat tissues except for ZW subcutaneous fat. The values in fat tissues were higher than those of other tissues at the same goose breed (p<0.01). Besides, average values in ZW fat and liver tissues were higher than those of Landes goose totally. These results suggested that the PPAR-γ expressions were positively associated with goose fat development and had a breed-related tendency. The PPAR-γ gene polymorphism could be used in Marker Assistant Selection (MAS) as a genetic marker for goose slaughter traits.
  Hua-Dong Yin , Zhi-Xiang Kuang , Hui-Ying Wang , Qing Zhu and Da-Qian He
  According to the public declaration by commercial companies, the commercial fatty liver line of Landes geese is grey feather. However, about 0.3% varieties of pure white or white with grey spots feather phenotype were observed in our purebred stock. So, we tentatively put forward a doubt whether some alien genetic components coexist in this commercial line. In order to prove this proposition, the mtDNA D-loop sequence variation were analyzed among 115 domestic goose individuals from this commercial fatty liver line and Chinese indigenous breeds. Total 11 haplotypes were determined and all, which could be classified into Asian haplogroup and European haplogroup. Seven landes geese residing in the E1-E3 haplotypes were clustered into the European haplogroup together with the wild greylag geese, while the remaining 45 Landes geese were grouped into the Asian haplogroup, which was predominantly occupied by the Chinese geese haplotypes. Although, the frequency (45/52) would be overestimated, we also reasonably believed that the matrilineal components of Chinese geese coexist in this commercial fatty liver line of Landes geese. In addition, the extremely low genetic diversity has been retained in the 2 sampled populations of Chinese geese, especially, in Wanxi white geese population.
  Hua-Li Wu , Xiao-Lin Zhao , Fan-Li Kong , Bing Zhou , Yao-Dong Hu , Juan Luo , Hui-Ying Wang , Qing Zhu and Da-Qian He
  PLIN plays a central role in the regulation of adipocyte metabolism which drives triacylglycerol storage in adipocytes and requires to hormonally stimulated lipolysis by cellular lipases. However, there is no report about the molecular structure and the expression profile of PLIN in goose. In this study, we cloned the goose PLIN gene and predicted bioinformatics. Quantitative Real Time PCR (qRT-PCR) assays were developed for accurate measurement of PLIN mRNA levels in Zhejiang White goose (ZW) and Landes goose different tissues from different ages (0-9 weeks). The results showed that goose PLIN cDNA sequence encoded an open reading frame of 527 Amino Acids (AA). The molecular phylogenetic tree among species were constructed that the mammal grouped together, Bombyx mori became another branch while the goose and chicken became another branch. It was found that PLIN mRNA were highly expressed in adipose tissues and to a lesser extent in cardiac and skeletal muscle and the difference is extreme significant between the fat and other tissues (p<0.01) yet, no expression in liver. In addition, there was a significantly age-related and breed-related change in fat tissues (p<0.05) and PLIN mRNA expression in ZW fat higher than that of Landes goose. All these results showed that the expression of PLIN mRNA in adipose tissues exhibited specific developmental changes, age-related and breeding patterns. The patterns of PLIN gene suggested that it played an important role in geese fat development. What’s more, further study is needed to reconfirm its function in a large population and in other breeds with different genetics background.
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