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Articles by D.K. Chen
Total Records ( 3 ) for D.K. Chen
  G.H. Zhao , W. Cheng , P.J. Zhang , Y.S. Han and D.K. Chen
  The present study analyzed genetic variation and genotypes of 17 strains of type 2 Porcine Circovirus (PCV2) from different epidemic regions in China in 2008 and 2009. All the genomic sequences were 1.767 bp in length. Sequence comparison of complete genomic sequences revealed 95.6-99.9% identity among 17 PCV2 strains and the most variable regions within 1.000-1.700 nt (located in the coding region of ORF2). Comparative analysis of amino acids of the two ORFs revealed that variation extend of ORF2 (93.1-100%) was greater than ORF1 (98.4-100%) and the third codon position showed much more variable than the first and second sites. Mutations in T and B lymphocyte epitopes were also detected by comparative analysis and it was found that T lymphocyte epitopes were more conserved than those of B lymphocytes. Phylogenetic analysis revealed 6 novel genotypes of PCV2 in addition to the 5 known geneotypes (PCV-2a, PCV-2b, PCV-2c, PCV-2d, PCV-2e) reported. Of these genotypes, the PCV-2b, PCV-2d and 3 unidentified genotypes were the most prevailing, within 13, 17 and 16 epidemic provinces, respectively. For the 17 Chinese PCV2 strains examined in this study, 5 strains represented PCV-2b genotype, 6 strains were PCV-2d, 1strain was PCV-2e and other 5 strains were novel genotypes while no strains were PCV-2a and PCV-2c genotype. These findings demonstrated the usefulness and attributes of complete genomic sequences for genetic variation and genotyping of PCV2 and have implications for the studies of population biology, molecular epidemiology and genetic structure of PCV2 and for the effective control of PMWS as well.
  C.Y. Wu , G.H. Zhao , Y.Q. Zhao , H. Liu , P.J. Zhang and D.K. Chen
  CD4+CD25+ T cells played a critical role in the establishment and maintenance of peripheral tolerance via adoptive transfer. However, whether one or more molecules in CD4+CD25+ T cells that could independently mediate peripheral tolerance was disputed by worldwide researchers. In the present study, one soluble antigen-specific factor was extracted from splenic lymphocytes lysates of OVA-tolerant mice (named OVA Immune Tolerance Factor, OVA-ITF) with molecular mass <3 ku which could establish OVA-specific immune tolerance in recipient mice via transfer treated and induce the same effect of peripheral tolerance as those of splenic lymphocytes from OVA-tolerant mice. Treated with OVA-ITF to naive BALB/c mice resulted in significant suppression of DTH reaction and T cell proliferation in an antigen-specific manner as well as a significant increase in the percentage of CD4+CD25+ T cells within the CD4+ T cell subset in peripheral blood. Present study showed that OVA-TIF was produced by CD4+CD25+ T cell subset and could induce OVA-specific peripheral tolerance independently in vivo with TGF-β1 as its main suppressive cytokine in recipient mice. These results suggested that OVA-TIF is a novel, low MW factor and totally different from other suppressive components reported previously which have important implications for expanding new potential therapeutic routes of prevention and control of graft rejection, autoimmune and related diseases.
  X.Q. Jing , Y.Q. Zhao , C.C. Shang , Y.L. Yao , T.T. Tian , J. Li and D.K. Chen
  Interleukin-17 is a critical pro-inflammatory cytokine in the development of autoimmunity and the immune responses against infection of bacteria, fungus and parasites. In the present study, dynamics of IL-17 and cytokines associated with IL-17 producing in serum and milk in experimental mastitis challenged with S. aureus and E. coli in dairy goats were monitored using commercial ELISA kits. The results showed that the levels of IL-17 in milk were peaked at 24 or 48 h post challenged with E. coli or S. aureus, respectively but no detectable peak was found in serum. The levels of TGF-β, IL-6 and IL-1β in milk were elevated in goats challenged with E. coli or S. aureus but only slight fluctuant were found in serum. These indicated that IL-17 was an important cytokine in the inflammation development of dairy goat mastitis challenged with E. coli or S. aureus and the local pro-inflammatory cytokines milieu plays an important role in the development of subclinical mastitis whether infected with E. coli or S. aureus.
 
 
 
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