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Articles by D.A. Astuti
Total Records ( 7 ) for D.A. Astuti
  R. Palupi , L. Abdullah , D.A. Astuti and Sumiati
  The aim of this research was to produce functional eggs that high antioxidant through substitution of soybean meal with Indigofera sp., top leaf meal in the laying hen diets. One hundred and sixty laying hens of Isa Brown strain, 30 weeks of age were kept into individual cages. Completely randomized design was used in this experiment, with 4 treatments and 4 replications. The treatment diets contained four kind combination of soybean meal (SBM) and Indigoferasp., top leaf meal (ITLM): T0 = diet contained 20% SBM and 0% ITLM; T1 = diet contained 17% SBM and 5.2% ITLM; T2 = diet contained 14% SBM and 10.4 ITLM; T3 = diet contained 11% SBM and 15.6 ITLM. The parameters observed were feed consumptions, egg production, egg quality and antioxidant activity. The results showed that substitution of soybean meal with Indigofera sp., top leaf meal not significantly (p>0.01) affected to feed consumptions, but significantly (p < 0.05) increased eggs production (83.63 to 92.65%), high significantly (p < 0.01) increased yolk colour, beta-carotene as well as vitamin A and decreased cholesterol content of the yolk. It was concluded that Indigofera sp., leaf meal could be used until 15.6% or substitute 45% soybean meal protein in the laying hen diets.
  T. Pasaribu , D.A. Astuti , E. Wina , Sumiati and A. Setiyono
  Sapindus rarak is a species of soapberry and in Indonesia the trunks commonly used as board cast, match sticks and wooden crafts and the saponin containing fruits are used as a detergent for traditional clothes (called batik). Saponin has biological activity to damage protozoa. The study has been carried out to investigate the effects of particle size and type solvent on the concentration of saponin extracted from the Sapindus rarak pericarp powder (SRP) and to evaluate the activity of SRP on oocysts of Eimeria tenella in vitro. Completely randomized design with 2 factors was arranged to analyze saponin content. First factor was type of solvent, i.e., 70% methanol and water. Second factor was particle sizes of SRP, i.e., 75, 300 and 600 μm and the saponin analyses of each treatment was done in triplicates. Determination of total saponin as described by Hiai and Nakajima and total sapogenin according to Hiai method with some modification. In vitro assay was performed at several concentrations of SRP in water (100-0.01 mg/mL). Results showed SRP with 75 μm particle size showed the highest foam height and the highest total saponin content compared to those having 300 and 600 μm of particle size. Solvent and particle size significantly affected saponin content. Saponin content of SRP was higher when it was extracted with 70% methanol (35.98%) than extracted using water (32.53%). SRP with 75 μm particle size had higher saponin content (43.52%) than those with 300 and 600 μm particle size (37.54 and 21.71%). Sapogenin content extracted with 70% methanol and water was not significantly different (30.55 and 30.36%), but particle size affected the sapogenin content. At 75 μm particle size, the sapogenin content was significantly higher (35.51%) than 300 and 600 μm particle size (33.46 and 22.40%). The SRP with concentration of 100-1 mg/mL water inhibited sporulation of E. tenella oocysts more than 90%. It is proved SRP has the ability to destroy the development of Eimeria tenella.
  A. Tarigan , S.P. Ginting , Arief II , D.A. Astuti and L. Abdullah
  Background and Objective: Indigofera zollingeriana leguminous have been known widely to have a concentrate feed characteristic due to its high nutrient contents (crude protein, vitamin and some mineral) and its highly dry matter (DM) digestibility. This study aimed to identify the effects body weight gain, nutrients degradability, fermentation rumen characteristics and blood metabolite of Boerka goat supplemented green concentrate pellets (GCP)based on Indigofera zollingeriana. Materials and Methods: Twenty four male Boer x Kacang crossbreeds with age of approximately male phase to 6 months and average initial body weight (BW) 13±0.5 kg were used in feeding and degestion trials. The study was assigned according to randomized block design with four dietary treatments and six goats were allocated to one of four treatments in randomised block design. The animals feed was offered chopped fresh Brachiaria humidicola (ad libitum) and feed treatments were offered daily at 4.0% body weight (BW). Results: The digestibility increased in line with the increasing proportion of I. zollingeriana in the green concentrate pellets (GCP). The tannin content of GCP seemed to not significantly impacted on feed intake. The daily body weight gain and efficiency of feed utilization increased as the proportion of I. zollingeriana GCP increased. Increasing of the proportion C. calotyhrsus in GCP affected the concentration of ammonia (NH3) and VFA of the rumen liquids of goat. Conclusion: Green concentrate pellets composing 90% I. zollingeriana gave the best results in term of daily body weight gain, feed intake, nutrient degradability, efficiency of feed utilization, rumen fermentation in Boerka goats.
  D.A. Astuti , L. Khotijah , M.S. Maidin and P. Nugroho
  Background and Objective: Reproduction system is affected by nutrient status of the animal. Flushing is one of reproduction program where the animal should give good quality diet. This study was aimed to evaluate etawah crossbred does reproduction performance giving flushing diet with different fat sources. The fat of plant oils are sunflower and flaxseed and from animal oils are tallow and Lemuru fish. Materials and Methods: Twenty four of Etawah crossbred does (average body weight 33.83±3.70 kg) were used in this experiment by using completely randomized block design. There are four treatments with four animals of each treatment. The treatments were flushing diet containing 5% sunflower oil (R1), 5.2% flaxseed oil (R2), 5.3% tallow (R3) and 5% Lemuru fish oil (R4). Treatment was given three weeks before and two weeks after matting, following 2 weeks before partus. During pregnant, the does were given basal diet (ratio concentrate:napier grass was 70:30). Body condition score, nutrient status, blood metabolite and hormone and also performance reproduction were evaluated. Results: The nutrient consumption was same in all treatment. Blood glucose were same in all treatments but the highest blood cholesterol was in R3 during estrus and in R4 during mid gestation. The highest plasma estradiol was in R1 during early gestation, while the highest plasma progesterone was in R2 during late gestation. Litter size and birth weight were same in all treatment, while the highest total embryo was in R2 treatment. Conclusion: It is concluded that flaxseed oil for flushing diet was significantly increased number of total embryo.
  R. Sriagtula , P.D.M.H. Karti , L. Abdullah , Supriyanto and D.A. Astuti
  Brown midrib sorghum (BMR) is a potential crop as forage because of lower lignin content than that of non-BMR sorghum. The aim of this research was to observe the growth and production of brown midrib sorghum mutant lines at different harvest times. This research was conducted at SEAMEO BIOTROP, Bogor, Indonesia using factorial in completely randomized block design (7 x 3) with three replicates. The first factor was the BMR sorghum mutant lines of Patir 3.1 (non-BMR/control), Patir 3.2, Patir 3.3, Patir 3.4, Patir 3.5, Patir 3.6 and Patir 3.7, the second factor was the harvest times (flowering, soft and hard dough phases). Measurement on agronomic parameters were fresh and dry matter biomass production, plant height, stem diameter, leaf width length and ratio of leaves, stems and panicles. While nutrient parameters were crude protein, crude fiber, ash dan crude fat production. Analysis of variance followed by Duncan Multiple Range Test (DMRT) was done. The results showed that the BMR of P 3.7 and P 3.2 produced the highest fresh and dry matter production among the BMRs, but their dry matter production were lower than P 3.1 (control). Harvesting at hard dough phase produce the highest fresh and dry matter production and as well as produced the highest crude protein, ash and crude fat production (p<0.01).
  N. Nurmeiliasari , R. Priyanto , D.A. Astuti , Salundik , J. Takahashi , M. Okamoto , T. Okamoto and A. Jayanegara
  Background and Objective: The effects of rumen mechanical stimulating (RMS) brushes on the health of Brahman crossbred steers were determined by measuring blood parameters and examining the rumen wall. Materials and Methods: This study used twenty Brahman crossbred steers divided into control and RMS groups. The steers were given low-forage diets (95.5% concentrate and 4.5% maize stover) based on 3% dry matter by body weight. The installation of RMS was conducted by a professional. Blood samples were collected at the end of the experiment from the coccygeal vein for blood profile and immunoglobulin G (IgG) measurements. The steers were slaughtered at the 93rd day of the experiment, samples of the rumen wall were collected and rumen histological observations were made. Results: The results showed that the RMS brush administration did not affect the hematocrit, hemoglobin (Hb), number of red blood cells (RBC), number of white blood cells (WBC) or IgG (p>0.05). However, there was a significant increase in neutrophils (p<0.05). The steers with RMS had a higher ratio of neutrophils to lymphocytes (p<0.05). Even though the treatment affected the neutrophil count and the ratio of neutrophils to lymphocytes, these values were in a normal range. The results of the rumen gross anatomy and histology observations showed that there was no tissue damage of the rumen, thus, it is in a normal state. Conclusion: The data indicate that the RMS brush utilization in steers does not result in adverse effects on the health of steers as measured by blood profiles, IgG concentrations and rumen histology.
  A. Bain , I.K.G. Wiryawan , D.A. Astuti , C. Arman and S. Suharti
  Objective: An in vitro study was conducted to evaluate the effect of soybean oil calcium soap (SOCS) and cashew fruit flour (CFF) supplementation on fermentability characteristics, microbial population and nutrient digestibility using rumen fluid of Bali cattle. Materials and Methods: The experiment was arranged in a complete randomized block design with 4 different ration treatments and 3 replicates. The ration treatments were R1: 40% native grass+60% concentrate, R2: 40% NG+60% C, containing 5% soybean oil and calcium soap, R3: 40% NG+60% concentration (C), containing 5% SOCS+10% CFF and R4: 40% NG+60% C, containing 5% SOCS+20% CFF. The measured variables were pH, NH3-N, total volatile fatty acids and total gas production (fermentability characteristics), total bacteria and protozoa (microbial populations), dry matter and organic matter digestibility. The data were analyzed using one-way analysis of variance and differences between treatments were examined using the Duncan’s multiple range test. Results: The results showed that the treatments significantly decreased (p<0.05) ammonia (NH3-N) concentration and total volatile fatty acids production. The different feed treatments did not have any significant effect on pH, total bacteria, protozoa population, dry matter and organic matter digestibility. Conclusion: Supplementation of 5% SOCS in R2 and 5% SOCS+10% CFF in R3 treatments has better NH3-N concentration, total VFA and total gas production compared to the other treatments.
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