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Articles by D.A. Ameh
Total Records ( 3 ) for D.A. Ameh
  D.B. James , D.A. Ameh and A.S. Agbaji
  Neem seed cake resulting after solvent extraction with water and different concentrations (35, 55, 75% and absolute) of methanol, ethanol and propanol were analyzed for crude protein, in vitro protein digestibility and amino acid composition Processed neem seed cake obtained from 55% methanol, 35% propanol and 100% propanol was significantly (p<0.05) lower in crude protein content compared to that obtained through water extraction while 75% ethanolic treated neem seed was significant (p<0.05) higher. There was a significant (p<0.05) increase in vitro protein digestibility of neem seed cake processed with 75% methanol over that with water. Neem seed cake obtained by extraction with 75% methanol, 75% ethanol and 55% propanol compared favorably with the amino acid profile to soya bean protein. The total essential amino acid content of neem seed cake obtained from 75% methanol was higher than that obtained for soya bean protein. The nutritional potential of neem seed cake, on the basis of these results, is discussed.
  M.A. Dakare , D.A. Ameh and A.S. Agbaji
  Biochemical assessment of pawpaw (Carica papaya) seeds and daddawa produced from the seed by fermentation was carried out. B. Subtilis, B. pumilus and B. licheniformis were found to be involved in the fermentation. The proximate composition showed that the seed had high lipid (48.50±0.45%) and protein (21.72±0.37%) contents, which increased significantly (p<0.05) after fermentation to 54.19±0.42 and 23.56±0.33% respectively. The main mineral elements found in fermented and unfermented seeds were magnesium, calcium and sodium. Fermentation decreased the level of antinutritional factors: oxalate from 210.1-40.2 mg/100 g, phytic acid from 102.0-68.0 mg/100 g, tannin from 15.5-8.3 mg/100 g and trypsin inhibitor from 2431.2-63.0 mg/100 g. Both fermented and unfermented papaya seeds were rich in the essential amino acids, leucine, lysine isoleucine and phenylalanine. Oleic acid is the predominant fatty acid in both raw and fermented seed oil being 77.7 and 80.7%, respectively, while, palmitic and stearic acids were present in appreciable quantities.
  A.C. Ene , S.E. Atawodi , D.A. Ameh , H.O. Kwanashie and P.U. Agomo
  The possibility of developing experimental chloroquine resistant Plasmodium berghei NK65 from chloroquine sensitive Plasmodium berghei NK65 was evaluated. Five mice of about 12 weeks old were inoculated with Plasmodium berghei (CQ sensitive strain). Exactly 72 h after inoculation and confirmation of parasitemia, these mice were treated with 10 mg kg-1 body weight (b.wt.) every 48 h for one month. After this period, treatment was withdrawn for one week, following which sub-inoculation was made from each of the five mice to four new mice for each group respectively. Seventy two hours after parasitemia was confirmed in the sub-inoculated mice, two of the four mice in each group were treated with the correct dose of chloroquine, that is, 25 mg kg-1 b.wt. daily for four days, while the rest were not treated. Parasitemia was monitored in all the groups for two weeks using thick and thin smears of blood films made from the tail vein of mice and stained with 10% Giemsa stain at pH 7.2. Two weeks after treatment with 25 mg kg-1 b.wt. dose of chloroquine was stopped, four mice died in the first two groups, while one mouse each died in the remaining three groups. Six of the untreated mice from the replicate groups equally died beyond two weeks, while four survived. At death, the % parasitemia of mice that died were higher than those that survived after 2 weeks. These results suggest that those mice that survived two weeks after treatment with the right dose of chloroquine (25 mg kg-1 b.wt. for 4 days) contained chloroquine sensitive Plasmodium berghei NK65 before they were cleared, while those that had persistence of parasitemia at relatively high level which resulted in their death contained chloroquine resistant Plasmodium berghei NK65. This finding should be of importance in studies involving development of new therapy for chloroquine resistant malaria.
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