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Articles by D. Silue
Total Records ( 2 ) for D. Silue
  A. Koudamiloro , F.E. Nwilene , D. Silue , A. Togola , O. Oyetunji , Y. Sere and M. Akogbeto
  Insects represent a major group of vectors of Rice yellow mottle virus (RYMV). This study aimed at identifying the main entomofauna transmitting RYMV to rice crop in Benin. Therefore, the transmission ability of 13 insect species was tested during the rice vegetative stages. These species belong to the Orthoptera order (Oxya hyla, Conocephalus longipennis, Paracinema tricolor, Acrida bicolor and Stenohippus aequus), the Coleoptera order (Chnootriba similis, Aulacophora foveicollis and Xanthadalia effusa), the Homoptera order (Cofana spectra, Nephotettix modulatus, Cofana unimaculata and Poophilis costalis) and the Diptera order (Diopsis thoracica). Among them, four species including P. tricolor, S. aequus, N. modulatus and P. costalis were identified for the first time as RYMV vectors. The species belonging to the Homoptera order appeared to be the most virulent, with higher values of viral titer. Virus distribution in the vector body parts was specific to each order. Basically, virus was more important in the Orthopteran, Coleopteran and Homopteran insects head part. It was also evident in the abdomen part of the Homopteran and the Orthopteran especially for P. tricolor, C. spectra and P. costalis. Disease severity was only observed in C. similis after inoculation and persisted until rice maturity with a score of 5 compared to the control which was 1 from a 1 to 9 scale. This study allowed identifying the presence of RYMV insect vectors in Benin among which, four are reported for the first time in Africa.
  J.T. Onwughalu , M.E. Abo , A.O. Nwankiti , J.K. Okoro and D. Silue
  The study was carried out to investigate pathotypes relationship of six Rice Yellow Mottle Virus (RYMV) isolates from North Central Zone of Nigeria in 2016 using some released Oryza species and Near Isogenic Lines (NILs). The experiment was laid out in a split-split plot design in the screen house at Badeggi, Niger State, Nigeria. Yield and agronomic data were subjected to statistical analysis using CropStat Version 7.2. The result showed a non-significant interaction effect of rice genotypes, disease isolates and inoculation regimes on spikelets sterility (%) and a highly significant effect (p = 0.01) on yield per plant (g) at harvest. The impact of the virus on the number of panicles per plant at maturity was significantly higher on test plants inoculated at active tillering stages which consistently produced lower mean panicle numbers across the levels of virus isolates. The result also showed that NIL 54 produced high resistance to the virus which is not due to immunity as the leaf extract from inoculated plant was infectious on susceptible FKR 28 during back inoculation test. NIL 54 did not also record significant reduction on the average leaf length (cm) per plant at maturity with the virus isolates; neither did it express the characteristic symptoms of the virus with the six isolates. Obubu-Ofu and Makurdi isolates circumvented the resistance in Gigante and showed obvious yellow mottle symptoms. The serological analysis and RYMV isolates characterization indicated that the six virus isolates belonged to sero groups 1 and 2 (S1 and S2). NIL 54 is recommended for further pathogenicity investigation with more isolates of RYMV in Nigeria or elsewhere, since, no obvious symptom of the virus was observed on the plant when challenged with S2 and S1 isolates in the present study.
 
 
 
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