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Articles by D. Ruswandi
Total Records ( 4 ) for D. Ruswandi
  H. Marta , E. Suryadi and D. Ruswandi
  Background: Maize is an important commodity in Indonesia for food and feed in food industry. The chemical composition and the genetics of Indonesian maize need to be identified for utilization as raw material in food industry. Materials and Methods: We evaluated nutritional composition of 45 single cross hybrids, 14 Indonesian maize inbred lines and 3 inbred testers following AOAC procedure. Combining ability estimate for chemical composition applied line×tester method. The F-test was used to test the significance of hybrids and combining ability mean squares. Results: The chemical compositions of Indonesian maize were varied. The protein ranged from 7.13-11.84% db, fat ranged from 2.58-7.17% db, carbohydrate ranged from 69.67-79.83% db, ash ranged from 0.95- 1.56% db, crude fiber ranged from 1.43-3.69% db. The good combiners for chemical composition of Indonesia maize were: DR 6 and DR 8 for carbohydrates, MDR 14.2.2 for protein, MDR 7.4.1 and DR 4 for fat, MDR 7.1.9 for crude fiber, MDR 9.1.3 and DR 8 for number of seeds per plant and MDR 9.1.3 for seed weight per plant. The superior hybrids were selected for chemical components. The superior hybrids are as follows: MDR 7.4.2×DR 6 for protein and crude fiber, MDR 14.2.2×DR 8 for protein and ash, MBR 153.7.1×DR 6, MDR 7.4.2×DR 4 for fat and carbohydrate, MDR 3.1.2×DR 4 for carbohydrate and ash. Conclusion: The chemical compositions of Indonesian maize were varied based on their genetic background. There are some good Indonesian maize parental combiners for each chemical composition traits and cross combination hybrids as well. These selected hybrids can be utilized in food industry.
  D. Ruswandi , A.L. Carpena , R.M. Lantican , D.M. Hautea , A.O. Canama and A.D. Raymundo
  Downy mildew caused by Peronosclerospora philippinensis (Weston) Shaw is one of the most persistent diseases encountered by maize producers in the Philippines. Improved durable resistant host plant could provide an effective way of both increasing and stabilizing corn production in affected areas. An extensive research to study the genetics of downy mildew resistance in maize against the UPLB isolate pathogen using generation mean analysis and to locate QTL conferring resistance against P. philippinensis causing Philippine downy mildew were done. The genetics of resistance to P. philippinensis was studied in progenies derived from crosses between the resistant inbred P 345 and Nei 9008 and susceptible inbred Pi 17 and Pi 23. Plant generations used in this study were the PS (susceptible lines); PR (resistant lines); F1, F2, F3, BS and BR. Analysis of generation means indicates that additive-dominance gene effect plays an important role in all components of resistance in all crosses involving Nei 9008. On the other hand, additive-dominance with epistatic gene effect plays an important role for all components of resistance involving P 345. QTL conferring resistance against P. philippinensis was observed in a BC1F2 population developed from a cross between Pi 23 and P 345. QTL analysis of the different components of resistance showed that a total of twenty-seven regions distributed in nine chromosomes of the maize genome were associated in varying degrees with resistance to P. philippinensis. Four regions located in chromosomes 1, 5, 6 and 8 were associated with disease incidence and disease severity. Results suggest that it may be possible to incorporate QTLs of various components of resistance into the elite inbred line Pi 23 using marker aided selection.
  D. Ruswandi , Agustian , E.P. Anggia , A.O. Canama , H. Marta , S. Ruswandi and E. Suryadi
  Drought stress is a very important factor in the reduction of maize production in Indonesia. A series of experiments were conducted to identify early maturing mutants, to study their genetic similarity and to select for mutants tolerant to drought stress. The first experiment was done in Jatinangor to identify early maturing second generation mutants (M2). The experiment was laid on augmented design with two replications in which fifteen M2 population groups with their nonmutant parentals were used as genetic materials. The second experiment was conducted in Biotechnology Laboratory, Institute of Plant Breeding, University of the Philippines at Los Banos to analyse DNA of 28 early maturing M2 using SSRs markers. The third experiment was conducted in Majalengka to select mutants tolerant to drought stress. The experiment was arranged in augmented design consisting of two replications of 18 nonmutant parental lines and 161 M3 which have been selected for early maturity. The last experiment was laboratory screening in the Plant Breeding Laboratory, University of Padjadjaran to confirm field drought tolerant mutants. The experiment was arranged in a Randomize Block Design (RBD) using 64 of M4 which showed early maturity. In the first experiment, there were 35 M2 that showed early maturity. Gamma irradiation was found to increase the phenotypic variation and diversity of plant height, ear weight, days to tasseling and days to harvesting. The SSR analysis was found to be a valuable DNA marker system to study genetic diversity of mutant and non-mutant lines. The mutant and non-mutant lines were clustered into two major cluster and seven sub-clusters based on a phylogenetic tree analysis using UPGMA. Based on the field screening for drought in the third experiment and confirmatory experiment in the laboratory, M3DR 18.8 and its progeny of M4DR 18.8.1 (selfing of 18.8) and M3DR 18.5 and its progeny, M4DR 18.5.1 (selfing of 18.5) are tolerant mutant lines as shown by their positive index of drought and their physiological response to water stress simulation using PEG. Those mutant lines could be considered for breeding program for tolerance to drought as an anticipation of global climate change.
  D. Ruswandi , J. Supriatna , N. Rostini and E. Suryadi
  Objective: Sweetcorn/chilli pepper intercropping is commonly applied to increase yield and profit by farmers in West Java, Indonesia. Methodology: An experiment was set up in Kutamandiri-Sumedang, West Java from January up to April, 2015 to select new sweetcorn hybrid and their parental lines for intercropping with chilli pepper. To evaluate the genetic materials, they were arranged by split plot design. This was replicated twice with the main plot consisting of four cropping systems, whereas, the subplots were 38 sweetcorn genotypes. Results: The result showed that parental line of MSR 17.2.3 is the best combiner for plant height, while SR 17 is the best combiner for maturity and ear weight per plant. The best combinations for high yield in both sole cropping and intercropping are MSR 12.6.7xSR 4 and MSR 25.5.1xSR 17. However, MSR 17.6.7xSR 17 were the best hybrid for intercropping based on land equivalent ratio, competitive ratio and stress tolerance index analysis. Conclusion: It is also identified that MSR 17.6.7xSR 17 was suitable for sweetcorn/chilli pepper intercropping for the following aspects including higher yield than sweetboy check variety both in sole and intercropping system, moderately level of competition ratio against chilli pepper, low tolerance index to chilli pepper and higher productivity in 2:1 sweetcorn/chilli pepper intercropping system.
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