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Articles by D. Puangpronpitag
Total Records ( 11 ) for D. Puangpronpitag
  D. Puangpronpitag and C. Sittiwet
  The aim of this study is to determine the antimicrobial activity of Cinnamomum verum stem bark aqueous extract against food-borne pathogen bacteria, nosocomial infection bacteria and normal flora. Extraction with an aqueous system from the dried stem barks of C. verum yielded 2.5% of the dried plant. Among 10 test strains of bacteria, C. verum showed inhibitory effect on the growth of Krebsilla pneumoniae ATCC 10031, Straphylococcus epidermidis ATCC 12228 and E.coli ATCC 25922 in an agar diffusion test. The Minimal Inhibitory Concentrations (MICs) and the Minimal Bactericidal Concentrations (MBCs) were in the range of 4-16 and 16-32 g L-1, respectively. In conclusion, C. verum stem bark aqueous extract showed interesting inhibitory effect on the growth of S. epidermidis, K. pneumoniae and E. coli at low minimum concentration. This may give additional information of antimicrobial activity of C. verum stem bark aqueous extract.
  D. Puangpronpitag , S. Chaichanadee , W. Naowaratwattana , C. Sittiwet , K. Thammasarn , A. Luerang and N. Kaewseejan
  The present study determined nutritional value in various parameters such as moisture content, carbohydrate, protein, lipid as standard methods and evaluated the antioxidative properties of the medicinal plant Gynura procumbens Extract by hydroxyl scavenging activity, ferrous ion-chelating activity and inhibition of lipid peroxidation. The highest extraction yield was obtained from extraction with distilled water (84.51%). While, Ethanolic Gynura extract (EGE) exhibited the highest in the antioxidative properties in every assay, EC50 = 1.63 in hydroxyl scavenging test, EC50 = 2.17 in chelating activity test and EC50 = 2.75 in inhibition of lipid peroxidation test. Although, the content still low (186.25±0.00018 to 300.18±0.0003 μg GAE g-1 of extract). According determination the nutritional values was found that Gynura procumbens Extract were containing the contents of moisture was 7.08%, carbohydrate was 0.0537 to 0.1968 μg glucose equivalent/100 g dry weight at EGE 0.1 μg mL-1, protein was 4.51 g/100 g dry weight and lipid was 0.023 g/100 g dry weight. These results suggest that Gynura Extract is a good protein source and that they may have positive effects on free radical scavenging and iron chelating which may use as preliminary information and develop further to be commercially useful in food industry or health products as medicinal food.
  N. Kaewseejan , D. Puangpronpitag and M. Nakornriab
  Gynura procumbens is one of the important medicinal plants in Thailand and Southeast Asia. It is usually used as a traditional medicine for the treatment of inflammation, herpes simplex virus, rashes, fever, rheumatism, kidney disease, migraines, constipation, diabetes mellitus, cancer and hypertension. The present study was to screen phytochemical compositions of ethanol extract of Gynura procumbens leaves such as chlorophylls, carotenoids, alkaloids, saponins, anthraquinone glycosides, volatile oils as standard methods and to evaluate the antibacterial activity by using agar well diffusion method. Phytochemical analysis revealed the contents of chlorophyll-a, b and carotenoids in ethanolic Gynura extract were 365.20±0.049, 132.40±0.029 and 53.20±0.034 μg g-1 dry weight, respectively. Moreover, the ethanolic Gynura extract showed the presence of alkaloids and volatile oils, whereas saponins and anthraquinone glycosides were absent. According to determination the antibacterial activity was found that Gynura procumbens showed as negative effects in all bacteria’s tested. These results suggest that Gynura extract is a good natural source of bioactive compounds and that they may have beneficial health effects for consumption which may use as preliminary information and could be further studied for uses in food industry, health product, pharmaceutical and medicinal applications.
  K. Chakuton , D. Puangpronpitag and M. Nakornriab
  Rice is one of the important foods of the world. Particularly, countries in Asia are popular eating rice daily food rather than in order regions of the world. Rice is sources of many bioactive non-nutrient compounds, known as phytochemicals. This study aimed to determine phytochemicals value such as, total phenolic compound, total anthocyanin, phytic acid, gamma (γ)-oryzanol composition and antioxidant activity of rice seed extracts of twelve colored and non-colored native Thai rice cultivars in Surin province, Thailand. The results showed that methanolic extracts of those rice seeds produced the highest extraction yield (5.45%). The colored cultivar 53 showed the highest Total Phenolic Content (TPC) and anthocyanins content by Folin-Ciocalteu method and pH-differential, respectively (7.40 mg Gallic Acid Equivalent (GAE)/100 g and 1045.12 mg malvidin/100 g, respectively). Moreover, there is a similarity in the γ-oryzanol content which detected by HPLC, colored rice seed extracts was statistically significant higher (p<0.05) than those non-colored. There were 4 major components of oryzanol (cycloartenol ferulate, 24-methylene cycloartenol ferulate, campesteryl ferulate and β-sitosteryl ferulate) which were successfully identified. Antioxidant activity of rice crude extracts was examined by DPPH scavenging method. Colored rice seed cultivar number 98 showed the highest activity (IC50 = 0.545 mg mL-1). While, phytic acid content in non-colored-rice seed cultivar number 236 showed the highest content (9.94 mg/100 g). From these results suggested to the potential of Thai rice seeds which may be further developed in food industry, cosmetic and health product.
  C. Sittiwet and D. Puangpronpitag
  The aqueous extract of Cryptolepis buchanani leaves was tested against food-borne pathogen bacteria (S. aureus ATCC 25923, E. coli ATCC 25922 and S. typhimurium ATCC 14028), nosocomial infection bacteria (K. pneumoniae ATCC 10031, P. vulgaris ATCC 13315 and Ps. aeruginosa ATCC 9721) and normal flora bacteria (L. plantarum ATCC 14917 and S. epidermidis ATCC 12228). The plant aqueous extract showed inhibitory effect against S. aureus ATCC 25923, E. coli ATCC 25922, S. typhimurium ATCC 14028, K. pneumoniae ATCC 10031, P. vulgaris ATCC 13315, B. subtilis ATCC 6633, L. plantarum ATCC 14917 and S. epidermidis ATCC 12228. The MICs (Minimal Inhibitory Concentrations) and MBCs (Minimal Bactericidal Concentrations) of this plant against all tested bacteria are in the range of 1-16 and 2-32 g L-1, respectively. In conclusion, C. buchanani leaves aqueous extract showed broad-spectrum antimicrobial activity against food-borne pathogen bacteria, nosocomial infection bacteria and some normal flora bacteria at low concentration. This may supported the used of C. buchanani aqueous extract as food-borne pathogen bacterial growth control additive and nosocomial infections treatment remedy.
  C. Sittiwet , N. Niamsa and D. Puangpronpitag
  The A. ebrateutus was extracted in boiling water with 0.7-1.3% yields. The antimicrobial activity of A. ebrateutus aqueous extract has been screened using agar diffusion method. A. ebracteutus aqueous extract showed inhibitory effect on growth of S. aureus ATCC 25923, S. epidermidis ATCC 12228, L. plantarum ATCC 14917, K. pneumoniae ATCC 10031 and P. vulgaris ATCC13315. The MICs and MBCs of A. ebracteutus has been evaluated using agar dilution and broth macro dilution methods. The MICs and MBCs of A. ebracteutus aqueous extract are in the range of 1-2 and 2-4 g L-1, respectively. In conclusion, A. ebracteutus aqueous extract showed good antimicrobial activity against nosocomial pathogen and skin infection bacteria at low concentrations. This might supported the used of A. ebracteutus to treat nosocomial infection and skin infections.
  C. Sittiwet and D. Puangpronpitag
  In this study, anti-bacterial activity of aqueous extract of P. igniarius against selected gram positive and gram negative bacteria using agar diffusion, broth macrodilution and agar dilution methods were investigated. The agar diffusion method revealed that P. igniarius aqueous extract showed inhibition zone against only S. aureus ATCC 24923. The susceptibility test against MSSA and MRSA revealed that P. igniarius aqueous extract showed inhibition zone against all of MSSA strains but cannot inhibit growth of MRSA. The broth macro dilution and agar dilution methods reveal MIC and MBC of P. igniarius against S. aureus ATCC 25923 was 1.25 and 2.5 g L-, respectively while MICs and MBCs against MSSA were 1.25-2.5 g L- and 0.25-0.5 g L-, respectively. The aqueous extract of P. igniarius showed inhibitory effect on growth of MSSA but not MRSA.
  C. Sittiwet and D. Puangpronpitag
  Anti-bacterial activity of P. gilvus aqueous extract was screened using agar diffusion method. The aqueous extract of P. gilvus showed inhibition zone against 3 out of 8 tested bacteria (L. plantarum ATCC 14917, E. coli ATCC 25922 and K. pneumoniae ATCC 10031). The MICs against L. plantarum ACC 14917, K. pneumonia ATCC 10031 and E. coli ATCC 25922 were 45, 90 and 360 mg L-1 respectively while MBC were 90, 180 and 720 mg L-1, respectively. The aqueous extract from P. gilvus showed good inhibitory effect against gram negative bacteria with low MIC and MBC.
  C. Sittiwet , D. Puangpronpitag and N. Niamsa
  The antimicrobial activity testing to evaluated the possibility of S. leucantha for treatment of nosocomial infection such as respiratory tract and urinary tract infections. The aerial part of S. leucantha was extracted using aqueous system with yield of 0.7-1.3% of dried weight of dried plant ’ s powder. The antibacterial activity of S. leucantha aqueous extract has been screened using agar diffusion method. The S. leucantha aqueous extract showed inhibitory effect on growth of L. plantarum ATCC 14917, E. coli ATCC 25922, K. pneumoniae ATCC 10031 and P. vulgaris ATCC 13315. The MICs of S. leucantha are in the rage of 8-16 g L -1 while MBCs are in the rage of 16-32 g L -1. In conclusion, the aqueous extract of S. leucantha showed inhibitory effect on growth of respiratory tract and urinary tract infection bacteria at low concentration. This result may give supporting data of used S. leucantha as nosocomial infection treatment.
  D. Puangpronpitag , N. Niamsa and C. Sittiwet
  Anti-microbial activity of Eugenia caryophyllum Bullock and Harrison aqueous extract has been tested against food-borne pathogen bacteria (S. aureus ATCC 25923, S. typhimurium ATCC 14028 and E. coli ATCC 25922), normal flora (S. epidermidis ATCC 12228 and L. plantarum ATCC 14917) and other pathogen bacteria (P. vulgaris ATCC 13315). The agar diffusion susceptibility test revealed inhibition zone of Eugenia caryophyllum Bullock and Harrison aqueous extract against S. aureus ATCC 25923, S. typhimurium ATCC 14028, E. coli ATCC 25922, S. epidermidis ATCC 12228, L. plantarum ATCC 14917 and P. vulgaris ATCC 13315. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by using agar dilution and broth macro-dilution methods. The MIC and MBC of clove against all tested bacteria were in the range of 1 to 4 g L-1 and 2 to 8 g L-1, respectively. In conclusion, the aqueous extract of E. caryophyllum showed good inhibitory effect on tested food-borne pathogen bacteria.
  C. Sittiwet and D. Puangpronpitag
  The objective of this study is to evaluate the antimicrobial activity of D. scandens aqueous extract against various bacteria. The stems of D. scandens were extracted using aqueous extraction with yield 1.5-2% of dried weight of plant stems. The antimicrobial activity was screened by using agar diffusion method. Minimum Inhibitory Concentrations (MICs) and MBCs values were determined using agar dilution method and broth macro-dilution method. The agar diffusion tested revealed that the plant aqueous extract showed inhibition zone against S. aureus ATCC 25923, S. epidermidis ATCC 12228 and E. coli ATCC 25922. Among three bacteria, S. aureus and S. epidermidis is gram positive bacteria, while E. coli is gram negative bacteria. Minimum Bactericial Concentration (MICs) of the plant aqueous extract were in range of 2-4 g L-1, while MBCs were in the range of 4-16 g L-1. In conclusion, the plant extract showed good inhibitory effect on growth of S. aureus and E. coli which are nosocomial infection bacteria and those S. epidermidis which is normal flora.
 
 
 
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