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Articles by D. Pandiaraj
Total Records ( 5 ) for D. Pandiaraj
  M. Leo Antony , D. Mubarak Ali , E. Baldev , D. Pandiaraj , R. Praveen Kumar and N. Thajuddin
  Fungi present in indoor environment always create serious health risks for the individual dwellers. The present study was focused on the enumeration of fungal contaminants in the newly constructed lab. Totally 8 different fungi were isolated from the indoor environment by using sedimentation culture plate method. The isolated species were identified conventionally, as well as using the sequence homology of internal transcribed spacer regions. This ribosomal non-coding unit sequence based analysis is the most popular locus for species identification and to study the phylogenetic variation. The fungal species isolated and described are already found to be reported as pathogens present in indoor air. Based on the sequences obtained, phylogenetic tree was constructed using both maximum likelihood and distance matrix depicts that the distance method using ClustalW2 offers better resolution and relates the related genera. The study reveals the methodology in fungal taxonomy and also for environmental monitoring of fungi.
  D. Vijayan , K. Manivannan , S. Santhoshkumar , D. Pandiaraj , M. MohamedImran , N. Thajuddin , K. Kala and M.H. Muhammad Ilyas
  The biodiversity study was executed to reveal the microalgal population in the Gundur Lake located in the southern part of Tiruchirappalli in Tamil Nadu, India. The samples were collected from the six chosen sites in the lake as algal mats and plankton samples during March 2014. In total, 81 species were reported which includes 35 species belongs to Chlorophyta, 9 species belongs to Bacillariophyta and 37 species belongs to Cyanophyta. Eight microalgal genera such as Pediastrum, Cosmarium rhophalodia, Microcystis, Chroococcus, Oscillatoria, Scytonema and Gloeotrichia were found predominant in the lake. Physicochemical parameters of the water samples were also analysed to evaluate the algal diversity. The suitable trophic condition and eutrophic nature of the lake favoured the algal dominance and bloom formation.
  D. Pandiaraj , D. Mubarak Ali , R. Praveen Kumar , S. Ravikumar and N. Thajuddin
  Most of the marine cyanobacteria especially to the order Chroococcales, Oscillatoriales, Nostocales occur ordinarily as planktonic forms. Their taxonomic assignment was based on morphological and cytological characteristics. The genetic variation in the species and strain level morphological and cytological features is not reliable and molecular characterization of cyanobacteria is necessary for better identification. The samples were collected from Thondi and Kattumavadi in Palk Bay region of Tamil Nadu, India. Biodiversity of cyanobacteria were documented, purified and maintained. The two strains, Phormidium chlorinum NTMP01 and Jaaginema psedogeminatum NTMP02 were selected for further molecular characterization based on 16S rDNA sequence in the strain. Evolutionary relationship and secondary structure was constructed with the sequence. The sequences were submitted to GenBank with accession numbers GU812856 and GU812857.
  A. Ilavarasi , D. Pandiaraj , D. MubarakAli , M.H. Mohammed Ilyas and N. Thajuddin
  Microalgal species are known to have pigments in their cellular constitute at the maximum and are valuable bioactive products. In the present study focused was on the evaluation of efficient extraction methods for photosynthetic pigments from microalgal species. They are, Chlorella sp., Acrochaete sp., Phormidium chlorinum, Jaaginema pseudogeminatum and Chroococcus sp. There are four different extraction methods were adopted for active recovery and are economically feasible such as direct extraction, mechanical grinding, heating and preheated solvent method. It was found that mechanical grinding method has extract two fold increased amount than the other methods. Additionally, this methods is inexpensive, less laborious and active extraction. It is suggested that this method could be used for the extraction of photosynthetic pigments from microalgae for pharmaceutical to biotechnological purpose.
  E. Baldev , D. MubarakAli , R. Shriraman , D. Pandiaraj , N.S. Alharbi and N. Thajuddin
  The present study focused on the extraction of cyanobacterial exopolysaccharides and their utilization as a bioflocculant. A cyanobacterium, Phormidium sp., was chosen based on the production of Extracellular Polymeric Substances (EPS). Later it was identified as Phormidium persicinum by 16S rDNA gene sequence and sequences were deposited in GenBank with accession number KC 859032. EPS was extracted from this strain using standard precipitation method. It was found that EPS production was maximum at late log phase of cyanobacterial growth (20 days). HPLC analysis revealed that presence of sucrose as a major component in the extracted EPS. Interestingly, the extracted EPS was found to be a good bioflocculant even at very low concentration (10 mg L-1). Cyanobacterial based EPS showed potential bioflocculation which can be directly utilized for the water purification and refining processes.
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