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Articles by D. Norouzian
Total Records ( 5 ) for D. Norouzian
  A. Akbarzadeh , D. Norouzian , A. Farhangi , M.R. Mehrabi , M. Bakhtiari , P. Afshar , A. Allahverdi , M.A. Mofidian and A. Shayan
  For the economic purpose of clinical trial and study of safety and immunogenicity of therapeutic morphine vaccine, 102 out of 200 outpatient volunteer addicts, whom were interested in abstinence, were injected with morphine vaccine, by randomized double blind method and under placebo control. The volunteers were divided into 3 cohorts, each consists of 30 subjects. The cohorts 1, 2 and 3 were injected with 12.5, 100 and 600 μg mL-1 of morphine vaccine, respectively. In each cohort, four additional subjects were injected with placebo. All the volunteers were bled prior to each injection and they got intra deltoid injections at 0-30-60 days and were monitored for safety and antibody production, for 12 months. All of 102 volunteers completed the course of three injections and all of them returned for the final scheduled visit at day 90th. The rise of antibody against morphine in all three vaccinated cohorts was controlled along the 5, 7, 9, 11 and 12 months. The vaccine was well tolerated with dose related increases in antibody levels and had no serious drug-related adverse events. Only 5 persons at the highest dose experienced brief post injection twitching. Anti-morphine antibody was detected by ELISA method after the first injection of 100, 600 μg mL-1 and second injection of 12.5 μg mL-1 doses and reached to its peak in 3 months and did not decline to baseline after one year. Thus, vaccine was well tolerated with dose related increases in antibody levels and a high proportion of outpatient volunteer addicts were recovered.
  A. Akbarzadeh , D. Norouzian , A. Farhangi , M. Mehrabi , M. Chiani , D. Zare , Z. Saffari , M. Mortazavi and A. Nikdel
  The effective constituent of human therapeutic morphine vaccine is morphine-6-succinate-BSA which would be produced by mixed anhydride method. By injection of 3 doses of vaccine at the interval of 0-30-60 days, humoral immunity would be caused in addicts. In this study 347 morphine addicted volunteers were vaccinated with therapeutic morphine vaccine according to WHO and national vaccination protocol. The variables were doses of vaccine, concentration of anti- morphine antibody, total protein and gamaglobuline. Volunteers were bled and then injected at the interval of 0-30-60 days. All subjects were bled at day 90 and after 1 year, 10% of them were bled randomly. Total protein and gamaglobuline were determined by serum electrophoresis and anti-morphine antibody level was estimated by ELISA. Considered variables were directly correlated with number of injections that were detected on 30 days after the first injection reaching their peak by three months after first injections and were not declined to the baseline by 1 year. All subjects were followed up and monitored for 1 year. The vaccine was well tolerated by addicted volunteers and had no serious drug-related adverse events. Only 1% at the first dose experienced brief post injection twitching and all subjects were immunized.
  D. Norouzian , A. Farhangi , S. Tolooei , Z. Saffari , M.R. Mehrabi , M. Chiani , S. Ghassemi , M. Farahnak and A. Akbarzadeh
  Bacterial Celluloses (BC) are gaining importance in research and commerce due to numerous factors affecting the bacterial cellulose characteristics and application in different industries. The aim of the present study was to produce bacterial cellulose in different media using different cultivation vessels. Bacterial cellulose was produced by static cultivation of Glucanacetobacter xylinum ATCC 10245 in different culture media such as Brain Heart Agar, Luria Bertani Agar /Broth, Brain Heart Infusion, Hestrin-Schramm and medium no. 125. Cultivation of bacterium was conducted in various culture vessels with different surface area. The cellulose membrane was treated and purified with a 0.1 M NaOH solution at 90°C for 30 min and dried by a freeze- drier at -40°C to obtain BC. The prepared bacterial cellulose was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD). The amount of produced BC was related directly to the surface area of culture vessels.
  M. Zarei , D. Norouzian , B. Honarvar , M. Mohammadi , H. Ebrahimi Shamabadi and A. Akbarzadeh
  Niosoms are nanoparticles used in drug delivery systems. Niosomes are prepared by various methods. In this research niosoms were prepared by reverse phase evaporation and the factors affecting the niosomes formation were studied. Percent of paclitaxel pegylated and non-pegylated prepared with Span 60 were 95 and 92, respectively while for those of pegylated and non-pegylated niosomes with Span 20, 94 and 90, respectively. In addition, the average diameters of pegylated and no-pegylated prepared with Span 60 and 20 were determined to be 191, 214, 244 and 284 nm, respectively. The amount of released drug (48 h) from pegylated and non pegylated formulations in the presence of Spans 60 and 20 were 8, 10, 6, 7%, respectively. Cytotoxicities of paclitaxel niosom polyethyleneglycol, paclitaxel niosome and free paclitaxel on MCF-7 cell line after 48 hours were studied by MTT assay. The results showed the formulation prepared with Span 60 is more effective than that of Span 20 and the IC50 of the former was decreased twice while IC50 of the later decreased 1.5 times.
  Q. Behzadiyannejad , S.D. Siadat , M. Kheirandish , B. Tabaraiee , H. Ahmadi , D. Norouzian , S. Najar Peerayeh , M. Nejati and M.H. Hedayati
  Production of effective vaccine formulations is dependent on the availability of assays for the measurement of protective immune responses. Antibody- and complement-mediated phagocytosis is the main defense mechanism against Neisseria meningitidis. Therefore, a newly developed phagocytosis assay based on flow cytometry (flow assay) and the Serum Bactericidal Activity (SBA) assay were using sera obtained from rabbit postvaccination with the Outer Membrane Vesicles (OMVs) of Neisseria meningitidis serogroup B was done in order to evaluation of the potential efficacy of (experimental) meningococcal vaccines. The OMVs were injected intramuscularly into of rabbits with boosters on days 14, 28 and 42 after the primary immunization. Phagocytic function of and intracellular oxidative burst generation by rabbit PMN, against Neisseria meningitidis serogroup B, was measured with flow cytometer (Coulter Epics-XL-Profile USA), using dihydrorhodamine-123 as probes, respectively. SBA titers are given as reciprocal Log 2 values of the dilution giving at least 50% killing of the inoculum measured as colony forming units. The results of SBA titers and quantitative flow cytometric analysis of rabbit PMN function in hyperimmun sera with the OMVs revealed a highly significant increase in opsonophagocytic responses and bactericidal antibody against serogroup B meningococci after 56 day (p< 0.05). Both SBA and opsonic activity are crucial for the protection against meningococcal disease. In conclusion, we have shown a very high correlation between opsonic activity and SBA (r = 0.91). Present results indicated that the OMVs could be as a candidate for vaccine toward serogroup B meningococci.
 
 
 
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