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Articles by D. Aziz
Total Records ( 5 ) for D. Aziz
  Aziz Arshad , S.M.N. Amin , Wan Nurul Izzah , D. Aziz and R. Ara
  Planktonic shrimp Acetes japonicus is found in the west of Peninsular Malaysia around the year. The study was conducted to observe the morphometric variation among the population of Acetes japonicus from four different sites along the coastal waters of Malacca, Penang and Perlis. Morphometric data of this species was analyzed using ANOVA and PRIMER software to investigate the degree of similarity among the populations of A. japonicus collected from four different sites (Malacca, Penang and two sides in Perlis) in the west coast of Peninsular Malaysia. The ANOVA showed that the mean difference of total length, standard length, carapace length and abdomen length amongst the four populations were highly significant (p<0.05). No significant difference was observed amongst the telson length of four populations (p>0.05). Dendrogram based on both sexes of morphometric characters showed three clusters amongst the populations. Sg. Berembang and Pantai Bersih populations were clustered in one group, Sg. Baharu population was in another group and both groups were well separated from the Klebang Besar population. The highest morphometric resemblance is observed between Sg. Berembang and Pantai Bersih populations which fall under the same group with a similarity of 99.67%. Analysis of variance showed that shrimp samples from the four sites differed significantly (p<0.05) based on morphometric characters.
  S.M. Al-Barwani , D. Aziz , S.M.N. Amin and A. Arshad
  Thirteen highly polymorphic microsatellite primer pairs developed for Perna viridis from the gene bank were tested on 3 populations of Perna perna samples with the aim to adapt a fast, reliable method for preliminary screening and to genetically characterize the wild populations of P. perna in the Oman waters. The samples were collected from three different locations viz Ras Al-Had, Ras Madrakah and Mirbat. All primers showed high level of polymorphism for all populations. The mean observed heterozygosity was lower than the expected heterozygosity across the three populations which means there was a probability of inbreeding occurred in the populations. Both the chi-square (χ2) and likelihood (G2) ratio tests detected significant differences (p<0.05), which showed deviations from Hardy-Weinberg equilibrium. Cluster analysis revealed a close genetic relationship of P. perna between all populations and they were clustered according to their geographical origins into two major groups which include one cluster that grouped the Mirbat and the Ras Madrakah populations together while the other cluster showed the Ras Al Had domain. The highest genetic distance (1.2666) was observed between the Mirbat and the Ras Al Had populations while the lowest genetic distance (0.4746) was recorded between the Mirbat and Ras Madrakah populations. This study demonstrated that microsatellite markers with thirteen P. viridis primer pairs tested can be applied to genetically characterize the brown mussel populations in Oman waters.
  D. Aziz , S.S. Siraj , A. Arshad , S.M. Nurul Amin and S.A. Harmin
  Genomic DNA of sergestid shrimp, Acetes japonicus was successfully extracted by using the Promega Wizard Genomic DNA Purification Kit. Population genetic characterization of A. japonicus along the western coast of Peninsular Malaysia (state of Perak, Malacca and Kedah) was examined using the Random Amplified Polymorphic DNA (RAPD) marker. Twenty oligonucleotides from operon A kit were used to screen the populations, of which 6 were able to be amplified (OPA03, OPA04, OPA07, OPA09, OPA10, OPA16). The percentages of polymorphic bands of the three populations investigated varied from 57.77 to 87.77%. Genetic distances between populations and cluster analysis from UPGMA grouped the populations into two major clusters. The Perak and Malacca populations were in one cluster, while the Kedah population was clustered by itself, indicating a different population. The genetic distance was highest as 0.0999 for the Kedah and the Malacca populations while lowest value was 0.0413 for the Perak and the Malacca populations, which probably have a closed ancestral relationship. The results of this study suggested that RAPD analysis, if carried out carefully and accurately would give a good indication of the separation between individuals of different populations and is suitable for identification of closely related genotypes.
  D. Aziz , S.S. Siraj , S.K. Daud , J.M. Panandam and M.F. Othman
  Information on the genetic diversity of Penaeus monodon throughout its natural range in Malaysia is still limited even though it is a highly exploited species, thus this study was undertaken to genetically characterize the prawn populations. The P. monodon samples were randomly collected from Malaysian waters and were characterized using thirty polymorphic primer pairs which showed high level of polymorphism. The total number of alleles per locus ranged from 3 to 36 with allele size ranging from 100 to 275 base pairs. The mean observed heterozygosity (0.5166) was less than the expected (0.5552), highly significant deficiencies in heterozygotes were detected in total inbreeding (FIS = 0.5500) and pair-wise genetic differentiation (FST = 0.6308) among the populations. Both the (χ2) chi-square and (G2) likelihood ratio tests detected significant differences (p<0.05) which showed a deviation from the Hardy-Weinberg equilibrium, indicating a probable inbreeding might have occurred in the populations. A Cluster analysis based on genetic distance revealed a fair genetic relationship among all the populations and the pattern was in accordance to the populations’ geographical origins. The highest genetic distance (0.7588) was observed between Lawas and Pulau Sayak populations while the lowest genetic distance (0.1191) was recorded between the Endau Rompin and Sedili populations. Various levels of genetic diversity of the P. monodon reported in this study indicated their genetic status in Malaysian waters and suitability for breeding and culture purposes. This information provides a basis for improvement through selective breeding and in the design of suitable management guidelines for this genetic material.
  A. Arshad , S.M. Nurul Amin , Y.L.Z. Nuradiella , Z.C. Cob , R. Ara and D. Aziz
  The study was an attempt to examine the size frequency distribution, length-weight relationship and morphometric variation of the Acetes japonicus from four different sides of Kedah coastal waters between March to May 2007 by using SPSS and PRIMER software. The average total length of the females from Batu Lintang and male of Tg. Dawai were the highest with the values of 17.07 and 13.93 mm, respectively. The growth co-efficient (b) values ranged between 2.0 to 4.0, indicating varieties of the growth pattern (negative allometric, isometric and positive allometric) for A. japonicus. The highest correlation of length-length relationship in female and male were found in the population of Kuala Sala. The morphological characteristics showed variation among populations. The highest similarity of morphometric characteristics was between Batu Lintang and Tg. Dawai for all groups (female, male, combined sexes).
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