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Articles by D. Sunil Kumar
Total Records ( 2 ) for D. Sunil Kumar
  Sathyanarayana N. Gummadi and D. Sunil Kumar
  Production of pectic lyases (PL and PGL) from Debaryomyces nepalensis has been optimized in submerged fermentation using Plackett-Burman design and response surface methodology. Four of the eleven fermentation variables (yeast extract, galactose, lemon peel and temperature) tested by Plackett-Burman design showed significant effect on both pectin lyase and pectate lyase production. The four screened variables were further optimized by central composite design. The optimal values of yeast extract, galactose, lemon peel powder and temperature for PL production was found to be 1.885, 0.956, 2.39% and 31.5°C and for PGL was at 2.5, 0.55, 2.4% and 32.7°C, respectively. The optimum activities of PL and PGL were found to be 10.73 and 8.73 U mL-1 after optimization which shows a 2.5 and 2.9 fold increase in PL and PGL production, respectively. This is the first time an optimization was done for production of PL and PGL by yeast Debaryomyces nepalensis. This could be an alternative to fungal pectolytic enzymes.
  Sathyanarayana N. Gummadi and D. Sunil Kumar
  Yeast isolates GSP 101, 102 and 103 capable of producing both pectin lyase and pectate lyase were isolated from rotten apple. Among this, isolate GSP 101 exhibited higher levels of pectin lyase and pectate lyase. Based on 26S rDNA D1/D2 sequence analysis, the isolate was identified as Debaryomyces nepalensis (99.8% sequence identity). The isolate produced maximum 3.2 U mL-1 of pectin lyase and 2.3 U mL-1 of pectate lyase when grown in basal medium supplemented with pectin (5 g L-1). The presence of glucose in medium did not show any negative effect on production of pectic lyases suggesting that there is no catabolite repression. Among the tested naturally available pectic substances, lemon peel was best inducer and carbon source for the production of PL and PGL. This is the first report on production of pectin lyase and pectate lyase by Debaryomyces nepalensis.
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