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Fungi present in indoor environment always create serious health risks for
the individual dwellers. The present study was focused on the enumeration of
fungal contaminants in the newly constructed lab. Totally 8 different fungi
were isolated from the indoor environment by using sedimentation culture plate
method. The isolated species were identified conventionally, as well as using
the sequence homology of internal transcribed spacer regions. This ribosomal
non-coding unit sequence based analysis is the most popular locus for species
identification and to study the phylogenetic variation. The fungal species isolated
and described are already found to be reported as pathogens present in indoor
air. Based on the sequences obtained, phylogenetic tree was constructed using
both maximum likelihood and distance matrix depicts that the distance method
using ClustalW2 offers better resolution and relates the related genera. The
study reveals the methodology in fungal taxonomy and also for environmental
monitoring of fungi.
