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Articles by Cui Wang
Total Records ( 5 ) for Cui Wang
  Shijun Li , Zhenhua Liang , Cui Wang , Yanping Feng , Xiuli Peng and Yanzhang Gong
  In the present study, we investigated the effect of squalene on the reproductive performance in meat-type male chicken. A total of 480 meat-type males, which were 72 weeks old with body weight of 6.0±0.5 kg were assigned randomly and equally to 4 groups in two independental experiments which represent two classic mating types AI (Artificial Insemination) and NM (Natural Mating), respectively. Each experiment contains two groups, control group and squalene-treated group in which meat-type males were fed with the supplemental squalene at a daily dose 10 mg/kg/day for 45 days. Semen data such as average collection semen volume, sperm count and egg fertile rate were collected from experiment I that was designed keeping males and females seperated and using artificial insemination as mating type. In the experiment I that designed keeping birds together in floor and using natural mating as mating type, only fertility data were collected. Also, serum testosterone levels were measured in squalene-treated, control and males no ejaculation groups. In the experiment I, the average collection semen volume is 393 μL, which is significantly higher (p<0.001) than the control group, which is 287 μL. The egg fertile rate has no significant difference between these two groups. In experiment II, the egg fertile rate of squalene treated group was significantly higher than control group (p<0.001).The serum testosterone levels of squalene treated group was significantly higher (p<0.05) than control group. The results showed that meat-type males treated with squalene increased the serum testosterone level and semen collection volume and did not decrease egg fertile rate in AI model and also increased the egg fertile rate in natural mating model. These findings have implications that squalene has a prompt effect on reproductive performance on meat-type male chicken.
  Zhenhua Liang , Cui Wang , Huawen Yu , Xiuli Peng , Yanping Feng , Yanzhang Gong and Shijun Li
  The tyrosinase family is known to be crucial in the melanin biosynthetic pathway and is responsible for the rate limiting step. In the present study, the complementary DNA (cDNA) of TYP1 was cloned from the eye of duck by homology cloning and rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of TYP1 consisted of 2123 nucleotides, containing an Open Reading Frame (ORF) of 1608 bp that encoding a 536 amino-acid peptide, a 5'-terminal Untranslated Region (UTR) of 255 bp and a 3'-terminal UTR of 260 bp with two canonical polyadenylation signal sequence (AATAAA) and a poly(A) tail. The phylogenetic tree display that TYP1 protein is highly conserved and the deduced peptide shares 70.9-93.7% similarity with quail, chicken and mammalian TYP1 proteins. The Semiquantitative RT-PCR analysis indicated that the transcripts of TYP1 mRNA had the highest expression in eyes and black hair follicle, intermediate in white hair follicle and negligible or absent in skin, muscle, heart, liver, kidneys, spleen, lungs, brain and intestine. The study may useful for the further study on polymorphism and correlation on duck feather color and the function of TYP1 of birds.
  Shijun Li , Wenhua Yu , Cui Wang , Zhenhua Liang , Xiuli Peng , Yanping Feng and Yanzhang Gong
  Stem Cell Factor (SCF) also called Steel Factor (SF), Mast cell Growth Factor (MGF) and KIT tyrosine Kinase receptor Ligand (KL) is a hematopoietic growth factor and ligand for the KIT tyrosine kinase receptor. In this study, the partial complementary DNA (cDNA) of SCF was cloned from the eye of duck by homology cloning and Rapid Amplification of Cdna Ends (RACE) approaches. Comparing with zebra finch, quail, chicken and mammalian SCF cDNA sequence, the phylogenetic tree displayed that duck SCF is highly conserved, it shares 63.8-94.3% similarity with the above species cDNA sequence. The rooted phylogenetic neighbor-Joining tree with bootstrap was done using SCF CDS sequences from duck and other species by DNAMAN software. The results showed that duck has close relationship with chicken, quail and zebro finch. The semi-quantitative RT-PCR analysis indicated that SCF was a universally expressed gene, it was detected in heart, liver, spleen, kidney, brain, back skin, muscle, eye, glandular stomach, belly skin. Q-PCR was employed to analyze the SCF gene expression in six type duck hair bulbs. The results showed that its expression had significant difference between W-W which is white hair bulb taken from white plumage with yellow beak and other types (BL-Bl which is black feather hair bulb taken from black plumage with black beak, W-L which is white feather hair bulb taken from white plumage with black beak ducks, W-WB which is white feather taken from white-black plumage with black beak, B-WB which is black feather taken from white-black plumage with black beak), p<0.001). The expression of SCF in BR (brown feather hair bulb taken from brown plumages with black beak ducks) has significant difference comparing with the above 5 hair bulb types (p<0.05). There is no significant difference between any of the following types which including BL-BL, W-L, W-WB and B-WB. The results indicated that SCF may be a critical gene on regulation gene expression for duck plumage diversity.
  Cui Wang , Yi Liu , Yunzhou Yang , Huiying Wang , Sifeng Yi , Chuang Li , Shaoming Gong , Weihu Chen and Daqian He
  MyoGenin (MyoG) is a basic Helix-Loop-Helix (bHLH) transcription factor that belongs to the Muscle-specific transcription Factors (MRFs) family which plays critical roles in regulating the skeletal muscle development and growth. In this study, the complete coding sequence and genomic DNA sequence of goose MyoG gene were cloned and characterized. The goose MyoG CDS was composed of 684bp that encoded a 227 amino acid protein, including a highly conserved basic helix-loop-helix domain. Multiple sequence alignments and phylogenetic analysis indicated that the deduced goose MyoG protein was conserved in vertebrates, especially in the avian species. The goose MyoG genomic DNA sequence we obtained was 3444bp and consisted of 3 exons and 2 introns.Sem-quantitative RT-PCR analysis demonstrated that the goose MyoG mRNA was specifically expressed in the breast muscle and leg muscle tissues, little or no expression was observed in heart, liver, spleen, lung, kidney, muscular stomach, brain, intestine and sebum. These data will serve as a foundation for further insight into the functions of the MyoG gene in Chinese domestic goose.
  Cui Wang , Yi Liu , Yunzhou Yang , Huiying Wang , Shaoming Gong , Weihu Chen and Daqian He
  Paired box (Pax) protein 3 is a member of the Pax family of transcription factors, plays critical roles in muscle development. Many studies showed that Pax3 gene was a functional candidate gene for production and meat quality. However, the sequence information of goose Pax3 gene remains unknown. In this study, the comparativegenomicstechnology was used to clone the cDNA sequence of Pax3 gene from the breast muscle tissue of Zhedong goose. The gene structures were analyzed by the bioinformatics software and the mRNA expression profile of Pax3 gene in different tissues was measured by the semi-quantitative RT-PCR. The goose Pax3 full-length coding sequence consisted of 1327 bp and encoded 421 amino acids. Sequence analysis displayed that a splicing variant of goose Pax3 absence of a glutamine residue was also identified. Both of the Pax3-a (inclusion of a glutamine residue) and Pax3-b (exclusion of a glutamine residue) isoforms were predicted contain three conserved domains (a paired domain, an octapeptide region and a homeodomain) and were highly conserved (>90%) relative to the known Pax3 proteins from other species. Multiple sequence alignments and phylogenetic analysis displayed that the deduced goose Pax3 proteins have a close genetic relationship and evolutional distance with the Pax3 proteins in other avian species, especially in ducks. The semi-quantitative RT-PCR results revealed Pax3 mRNA was highly expressed in the breast muscle tissue, followed by the lung, leg muscle and brain, little or no expression was observed in heart, liver, spleen, kidney, muscular stomach, intestine and sebum. These findings will help us understand the functions of the Pax3 gene and the molecular breeding in Chinese domestic goose.
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