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Articles by Ciler Meric
Total Records ( 2 ) for Ciler Meric
  Ciler Meric , Feruzan Dane and Goksel Olgun
  In this study, development of anther wall of Helianthus annuus L. male fertile HA 89 “B” line and cytoplasmic male sterile HA 89 “A” line were compared by light and electron microscopy. It was observed that there was no difference between male fertile HA 89 “B” line and male sterile HA 89 “A” line development of anther wall until tetrads were formed. After tetrad stage in male fertile HA 89 “B” line, middle layer was lost and tapetum was parted from anther wall and surrounded the microspores. In cytoplasmic male sterile HA 89 “A” line middle layer became vacuolated and widened, tapetum also enlarged and filled anther sac. Besides it was determined that middle layer and tapetum were permanent with epidermis and endothecial cell during the further development of anther wall and also there was no secondary thickening in endothecial cells. In conclusion we suggest that the plasmodial tapetum which developed in fertile HA 89 “B” line transferred nutrients to microspores and helped them develop normally, but in cytoplasmic male sterile HA 89 “A” line the plasmodial structure did not develop. The inner tangential wall of tapetum, which was adjacent to the tetrads did not degenerate, so that sufficient nutrients were not transferred to microspores and thus they degenerated while in their callose walls.
  Ciler Meric , Necmettin Guler , Hayati Arda and Sergun Dayan
  Nuclear DNA content of Trachelium jacquinii subsp. dalgiciorum, an endemic taxon, was analysed using flow cytometry. Plants were collected from a natural population. While some plants were prepared as herbaria material, others were placed in pots and incubated in growth chamber. Fresh leaves for flow cytometry analysis were chopped in MgSO4 buffer (with dithiothreitol, propidium iodide and triton x-100) on ice in a Petri dish and stained with propidium iodide (with RNase-DNase free). Stained nuclei were analysed on an EPICS XL (Beckmann Coulter) model flow cytometer. The mean DNA content per plant was based on 10000 scanned nuclei. Nuclear DNA content (2C-value) of Trachelium jacquinii subsp. dalgiciorum was found as 3.44±0.14 pg (1C-value 1.72 pg). This study contributes present data on nuclear DNA content of angiosperm taxa.
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