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Articles by Chunlei Zhang
Total Records ( 2 ) for Chunlei Zhang
  Chunlei Zhang , Bo Liu , Hong Chen , Xianyong Lan , Chuzhao Lei , Zhiqing Zhang and Runfeng Zhang
  The objectives of the present study were to estimate the allele and genotype frequencies of the POU1F1/HinfI polymorphisms in beef cattle belonging to four different genetic groups and to determine the effects of these polymorphisms on growth traits in cattle. The 451-bp PCR products of POU1F1 gene digested with HinfI exhibited three genotypes and two alleles, which were at Hardy-Weinberg equilibrium (P>0.05). Genotype BB was the predominant genotype and B the predominant allele in the studied populations. There was significant difference between Limousin x Qinchuan and Qinchuan in the distribution of genotypes (P < 0.0001). The association of the polymorphism of the POU1F1 gene with growth traits among Qinchuan, Limousin x Qinchuan, Angus x Qinchuan and Germany Yellow x Qinchuan crosses was analyzed. Body weight and wither height of individuals with genotypes AB were higher (P < 0.05)than that of individuals with genotype BB in the Germany Yellow x Qinchua cross, but were not in the other three populations (P>0.05).
  Zhuye Niu , Chunlei Zhang and Fuzhu Liu
  Three experiments were carried out to determine optimal feed input and excreta collection time in bioassay of feedstuff true metabolizable energy for ducks. In experiment 1, 20 drakes had been food withdrawn and were allocated to 4 groups, each of which contained 5 birds. Birds of each group were force-fed 50 g complete feed, approximately 48 h after food withdrawal. The excreta voided during the exact 56 h following feeding were collected. In experiment 2, 28 drakes were force-fed 50 g complete feed, approximately 48 h after food withdrawal. 4 birds were killed by an intravenous dose of sodium pentobarbitone 4 h, 8 h, 14 h, 24 h, 32 h, 38 h and 48 h after feeding. The residues in alimentary canal were collected and dried in an oven at 65 °C. In experiment 3, 70 mature drakes were allocated to 7 groups. The treatments comprised an unfed negative control and five levels of feed input ( 30, 50, 70, 90, 110 and 150 g complete feed). The birds were force-fed at approximately 48 h after food withdrawal. The excreta voided during the exact 36 h following feeding were collected. The results indicated that the time prior to force-feeding and excreta collection period should be 32-36 h when evaluating feed metabolizable energy and 50-70 g feed per bird is feasible for evaluating feed metabolizable energy.
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