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Articles by Chunjiang Zhao
Total Records ( 7 ) for Chunjiang Zhao
  Yonggang Shao , Changxin Wu , Junying Li and Chunjiang Zhao
  In this experiment, forty triplets consisting of full-sib Tibetan Chicken cockerels were divided equally into two trial groups. In each group, the triplets were randomly assigned to caponization, sham treatment and intact groups. The birds of the two trials were caponized or sham-operated at either 6 weeks of age (early) or 18 weeks of age (late) and slaughtered at 24 weeks of age. The birds in the early caponization group showed significant increases in terms of intermuscular fat deposits, subcutaneous fat thickness, liver weight, triacylglycerol concentration (p<0.05) and abdominal fat weight (p<0.01) at 24 weeks of age compared with the intact and sham groups, while later caponization resulted in significant increase in liver weight, abdominal fat weight, total cholesterol and triacylglycerol concentrations (p<0.05). In both trials, the capons exhibited lower leg muscle weight than did the intact (p<0.05). There were no significant effects on breast muscle weight on either the early or late caponization group. We concluded that late caponization accelerates the rate of fat deposition within the abdominal cavity compared to other areas after sexual maturity. Present results also suggest that the role of androgen on the growth of breast muscle is different from that on leg muscle in Tibetan Chicken cockerels. It seemed that the positive effects of androgen were reflected only on leg muscle growth.
  Bo Yu , Huojun Cao , Chunjiang Zhao , Changxin Wu and Xuemei Deng
  Map-based positional cloning of Drosophila melanogaster genes is hampered by both the time-consuming, labor-intensive, error-prone nature of traditional methods for genetic mapping-SNP mapping is costly and P element mapping is low-resolution for limit number of P elements. Here we used two strategies to locate a spontaneous mutant screened in our lab. One is P element mapping to map the mutant in a 33 k-interval on the right arm of the second chromosome; another is combination of P element mapping and a Single-Nucleotide Polymorphism (SNP) mapping together to map the mutant in a very small interval, about 5 kb, on the right arm of the second chromosome efficiently. At last, we guessed that the mutant phenotype maybe resulted from the overexpression of twist gene. Comparing the two strategies, the method combining the P insertion mapping and SNP mapping could ward off their drawbacks. Therefore, the strategy of combination of the two methods provides a rapid protocol for mapping mutations.
  Hongwei Li , Chunjiang Zhao , Haiyue Xu , Bo Yu and Changxin Wu
  Classic taxonomy of animal mainly depends on genetic, morphology, fossil and distribution studies, so the study aims at developing a method which can rapidly identify to what species unknown animals are closely related by comparison of genomic sequences. With more complete sequenced animal genomes available, genomic information will play a key role on classifying unknown animals. Here, we put forward a method how to rapidly identify unknown animals. The method includes: (1) selecting a vector which is convenient for cloning and sequencing; (2) digesting the total genomic DNA from unknown animals and the vector with the same two enzymes which have recognition sites on the vector; (3) ligating the digested DNA to the vector; (4) transforming the ligation products to competent cell; (5) selecting positive clones to sequence and blast with sequenced animal genomes; (6) then finding out the most close relative species to the unknown animals. In the paper we took a kind of unknown wild flies which captured in nature, for example, to display the method. By analysis of data from blast output, results showed that eleven query sequences (1, 2, 4, 5, 6, 7, 9, 10, 12, 13, 14) from unknown flies have the highest similarities with subject sequences on the D. ananassae genome, but only minor parts of No. 2, 4~7, 9, 10 and 12 sequences have similarities with subject sequences on eleven drosophila genomes and the unknown flies are the most closely related to D. ananassae in evolutionary contexts. it will be a promising power in rapidly identifying to what species unknown animals is closely related when more complete sequenced animal genomes are available .
  Hongwei Li , Linsheng Lian , Chunjiang Zhao and Changxin Wu
  The Cy mutation is the most commonly used dominant marker for the second chromosome balancers in Drosophila melanogaster, but little is known about molecular mechanism underlying the Cy phenotype. So, the study aimed at detecting the difference at DNA level between the curly stocks and the wild type by PCR amplification and sequencing. Here we combined genomic and cytological information to detect difference at DNA level between curly stocks and the wild type. Firstly, by sequence analysis a 102 bp deletion which locates the genomic interval between genes syt and daw has been found to be associated with the Cy mutant chromosome. Using the deletion as a marker, we detected genotypes of flies from different developmental stages in Cy67.3: embryo, the first star larva, the second star larva, the third star larva and pupa. Results showed that all flies with genotypes BB and AA have been dead before adults. At DNA level these results provided a powerful proof for a conclusion that the curly maintains itself in a balanced lethal stock. In addition, a prediction suggests that two transcription binding sites which contain TAAT or ATTA, core recognition sequences of homeoproteins in drosophila melanogaster, are located in the region in which the deletion occurs. So, it is interesting for better understanding the molecular mechanism underlying curly phenotype.
  Dan Du , Chunjiang Zhao , Hao Zhang and Guocai Han
  Tibetan Horse lives in Qinghai-Tibet Plateau of South-West China, the controversy on whether Tibetan Horse should be classified into Southwest Horse Type or not has existed in China for a long time as there are four other type horses in the country. In the study, the genetic diversity and structure of Tibetan Horse were investigated using 14 microsatellite markers and 153 animals belonging to three Tibetan Horse subpopulations in Xigaze, Qamdo and Nagqu, respectively and Mongolian Horse and Ningqiang Pony which served as reference groups to Tibetan Horse. The results showed that the mean Number of Allele (MNA) across the 14 loci in Tibetan Horse was 13.1 and the average observed heterozygosity (0.710) and PIC (0.752) were slightly higher than those (0.692 and 0.773, respectively) of Mongolian Horse. Although, there was abundant genetic variation, genetic differentiation between horse populations was low, with only 2.1% of the total genetic variance among populations. Qamdo, Xigaze and Mongolian Horse had close relationship and Nagqu is distinctly separated from the other two Tibetan Horse subpopulations and referenced breeds in the clusters. The results indicated the genetic relationship of Tibetan Horse were closer to Mongolian Horse rather than to Ningqiang Pony, a typical breed in Southwest Horse Type, which suggests that Tibetan Horse was a unique horse breed which could not be classified into Southwest Horse Type.
  Yiming Sulaiman , Changxin Wu and Chunjiang Zhao
  To study genetic information of the local sheep populations of Northwestern China and their phylogenetic positions in relation to other Asian populations and European sheep, the genetic diversity and phylogeny evolution of 223 individuals from 19 indigenous sheep populations in Northwestern China and one Western breed, Texel were analyzed with entire sequences of mitochondria DNA control region. The length of the sequences was considerably variable between 1103 and 1256 bp. Phylogenetic analysis indicated that 3 mtDNA lineages A, B and C were found in all sampled populations, except for the absence of lineage C in two populations. Phylogenetic tree and median joining network were constructed with the sequenced mtDNA control region and both the phylogenetic tree and network demonstrated that there were three independent clusters and some sequences deviated from cluster A and C. Neighbor-joining tree constructed based on genetic distances showed a multi-maternal originality of the nineteen Xinjiang local sheep populations, which were clustered with the sheep groups in other parts of China, Central Asia, Turkey and India. The high genetic diversity of Xinjiang local sheep populations suggests that they may have distinct maternal origins and more complicated phylogenetic history than the other Chinese sheep breeds.
  Feng Huang , Chunjiang Zhao , Feifei Li and Huarui Wu
  The large scale farmland WSN monitoring has the characteristics of wide area and energy constraint. Nodes around sink node undertake lots of data transfer work, so these nodes have the problems of excessive energy consumption and some of them are inclined to early death. This study gives an improved a topology control algorithm for farmland wireless sensor networks based on Yao Graph topology and clustering algorithm. The improved algorithm is aimed to balance the nodes energy in the critical region and prolong the network lifetime by clustering and energy regulatory in the sink node communication range. The simulation results shows that this topology control algorithm is conductive to energy balance in the communication key areas-sink node communication range and at last prolong the nodes and the network life time.
 
 
 
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