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Articles by Chun Zhang
Total Records ( 4 ) for Chun Zhang
  Chun Zhang and Biyu Zhang
  Considering multivariable and close coupling of asynchronous motor vector control system, an intelligent control strategy named nine-point five-state controller was adopted as speed controller. The intelligent genetic algorithm (IGA for short) was applied to optimize the controller parameters, which used orthogonal experiment and intelligent mating operations to improve the optimization performance. The detailed optimization method and process were given. Simulation results have indicated that this kind of strategy can not only improve system dynamics and steady-state behavior but can also achieve relatively strong anti-disturbance performance.
  Haiqing Yu , Chunbang Ding , Chun Zhang and Yonghong Zhou
  In order to obtain more cytological data, the karyotypes of Pseudoroegneria gracillima and P. kosaninii were investigated. Root tips of P. gracillima and P. kosaninii were pretreated in an ice bath, fixed in a mixture of 95% ethanol: glacial acetic acid and treated in 1 M HCl at 60°C in a water bath. Somatic cells were stained in Schiff at room temperature and the meristematic portions of the root tips were squashed in 45% acetic acid. The results show that: (1) P. gracillima is diploid with two pairs of satellites and P. kosaninii is octoploid with three pairs of satellites. The karyotypes of diploid P. gracillima and octoploid P. kosaninii are first reported, (2) the karyotype formulas of P. gracillima and P. kosaninii are 2n = 2x = 14 = 12 m (2sat)+2sm (2sat) and 2n = 8x = 56 = 42 m (6sat)+12sm+2st, respectively and (3) the karyotype of P. gracillima is 1A type, while P. kosaninii is 2B type. This demonstrated that there are great variations between the karyotypes of P. gracillima and P. kosaninii.
  Haiqing Yu , Chun Zhang , Zhiqiang Mei , Li Wang , Juan Li , Chenggang Duan , Xiaoyan Liu , Shu Gong , Lin Gan , Zhonghua Tao , Fuli Yao , Ye Yang , Chunyan Duan , Youping Liu , Chunyan Zhang , Chuanning Chen , Xiaoli Zheng , Hongxian Zhao , Jiyan Cheng , Xiaojun Tang , Yong Zhang , Yingxi Zhang and Junjiang Fu
  As a traditional medicinal herb in China, Penthorum chinense Push from Gulin county is regarded as genuine regional drug with better clinical effects. To discriminate the geographical origin of six P. chinense samples cultivated in three provinces, Randomly Amplified Polymorphic DNA (RAPD) analysis was carried out with an improved method to increase the resolution and production using 10 mer-random primers. Similarity index was ranged from 0.61 to 0.97, which demonstrated that samples from different localities displayed similar band patterns. However, based on the analysis of selected 13 primers, primers SBS-I9, SBS-I20 and SBS-Q9 produced distinguishable bands among Sichuan, Yunnan and Hubei P. chinense. T-test of mean S.I. values of six accessions demonstrated the significant differences between Luzhou or Sichuan samples and others. Cluster analysis indicated that cultivars with close geographic distributions were clustered together consequently. This suggested that there are RAPD site variations and local specialized genotypes among the six samples. The results indicated that RAPD analysis is effective in distinguishing the geographical origin of genuine regional drug P. chinense grown in Luhzou, Sichuan. The approach is a valuable tool to authenticate other morphologically similar herbal medicinal materials.
  Guli Asa , Chun Zhang , Yonggang Wang , Meng He , Yilong Yin , Xiping Fan and Yanming Wei
  A rapid, sensitive and high throughput micro-plate method was established to quantify polysaccharides in this study. Anthrone-sulfuric acid micro-plate assay in a certain reaction was tested. A single test and a response surface methodology was applied to design and optimize four factors including reaction time, incubation temperature, anthrone concentration and the amount of anthrone in order to maximize R2 and minimize RSD in each standard curve which obtained in different conditions. It was demonstrated that the order of significant impacting R2 is incubation temperature, anthrone concentration, reaction time and the quantity of anthrone add to while the order of significant impacting RSD is incubation temperature, anthrone concentration, the amount of anthrone and reaction time. Preferred reactive condition is be discovered and the best incubation temperature is 96°C, the best reaction time is 36 min, anthrone concentration is 1.1 mg mL-1 and the amount of anthrone is 150 μL. The detectable range of this method is 20-260 μg L-1 under the optimized condition. Recovery test showed high accuracy and practicability in this method by recovery rate ranged in 99.5-100.6%. The method was successfully applied to quantify polysaccharides of different source and the method has high resistance to interference of protein during measurement process.
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