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Articles by Chuanbin Zhu
Total Records ( 2 ) for Chuanbin Zhu
  Chuanbin Zhu , Xingzhu Qi , Canding Wang , Wuzhou Yuan , Weiqi Zeng , Yuequn Wang , Zaochu Ying , Yun Den , Mingyao Liu , Yongqing Li and Xiushan Wu
  SVOPs are synaptic vesicle proteins that contain 12 transmembrane regions. Herein, we have isolated a novel human homologue of SVOP gene from a human brain cDNA library. This gene contains an open reading frame of 1646 nucleotides, coding for a protein with 548 amino acids. hSVOP is highly conserved in evolution across different species from C. elegans, fruit fly, mouse, to human. Northern blot analysis showed that it is expressed specifically in human brain. Transient transfection of COS-7 cells with hSVOP-EGFP demonstrates that hSVOP is located on cytoplasmic membrane and may be involved in material transfer between the synaptic vesicle and cytoplasm.
  Weiqi Zeng , Yun Deng , Zhiyuan Yang , Wuzhou Yuan , Wen Huang , Chuanbin Zhu , Yan Bai , Yongqing Li , Zhonglu Peng , Yuequn Wang , Ying Zhu , Mingyao Liu and Xiushan Wu
  The calcium chloride method is an efficient and convenient technique to prepare Escherichia coli competent cells. The transformation efficiency of the competent cells when prepared in the laboratory is generally below 106 cfu μg-1 (colony forming units μg-1) DNA and thus its application is limited in some extent. Here we report the utilization of a-SiNPs (amino modified Silica-Nanoparticles) as a help material to improve the transformation efficiency of E. coli competent cells that are prepared with calcium chloride method. Addition of a-SiNPs increases the transformation efficiency of E. coli to 107-108 from below 106 cfu μg-1 DNA in the laboratory.
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