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Articles by Christopher E. Jones
Total Records ( 2 ) for Christopher E. Jones
  Karen H. L. Lim , Christopher E. Jones , Rachel N. vanden Hoven , Jennifer L. Edwards , Megan L. Falsetta , Michael A. Apicella , Michael P. Jennings and Alastair G. McEwan
  mntABC from Neisseria gonorrhoeae encodes an ABC permease which includes a periplasmic divalent cation binding receptor protein of the cluster IX family, encoded by mntC. Analysis of an mntC mutant showed that growth of N. gonorrhoeae could be stimulated by addition of either manganese(II) or zinc(II) ions, suggesting that the MntABC system could transport both ions. In contrast, growth of the mntAB mutant in liquid culture was possible only when the medium was supplemented with an antioxidant such as mannitol, consistent with the view that ion transport via MntABC is essential for protection of N. gonorrhoeae against oxidative stress. Using recombinant MntC, we determined that MntC binds Zn2+ and Mn2+ with almost equal affinity (dissociation constant of ~0.1 µM). Competition assays with the metallochromic zinc indicator 4-(2-pyridylazo)resorcinol showed that MntC binds Mn2+ and Zn2+ at the same binding site. Analysis of the N. gonorrhoeae genome showed that MntC is the only Mn/Zn metal binding receptor protein cluster IX in this bacterium, in contrast to the situation in many other bacteria which have systems with dedicated Mn and Zn binding proteins as part of distinctive ABC cassette permeases. Both the mntC and mntAB mutants had reduced intracellular survival in a human cervical epithelial cell model and showed reduced ability to form a biofilm. These data suggest that the MntABC transporter is of importance for survival of Neisseria gonorrhoeae in the human host.
  Lin Zhang , Narsimha Reddy , Cheang Soo Khoo , Sundar Rao Koyyalamudi and Christopher E. Jones
  Background and Objective: Lobelia chinensis Lour is an important anticancer herb used in traditional Chinese medicine. Many botanical polysaccharides are known to exhibit immunomodulatory and anticancer activities. This research aimed to analyze the L. chinensis polysaccharides (LCPs) for their biological activities relevant to their anticancer function. Materials and Methods: Water-soluble LCPs were extracted and purified using size-exclusion chromatography to obtain two dominant polysaccharides, LCP-1 and LCP-2 having molecular masses of 1899 kDa and 5.3 kDa, respectively. The antioxidant potentials of the isolated polysaccharides were evaluated by measuring radical scavenging activities against DPPH (2,2-diphenyl-1-picrylhydrazyl radical), ABTS∙+(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid radical) and OH(hydroxyl radical). Immunostimulatory activities of LCP-1 and LCP-2 were measured using mouse macrophages. Structure of the most active fraction (LCP-2) was determined using FT-IR and NMR spectroscopic techniques. Results: Two isolated polysaccharide fractions displayed significant antioxidant activities and stimulated the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), although LCP-2 is more effective. Detailed structural characterization by FT-IR and NMR was undertaken for the most active fraction (LCP-2) and confirmed that LCP-2 is(2,1)-β-fructan. Conclusion: The results suggested that the polysaccharides isolated from Lobelia chinensis Lour are potential candidates for immune-chemotherapy and suitable for the treatment of cancer.
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