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Articles by Chong Wang
Total Records ( 2 ) for Chong Wang
  Yingjie Mei , Yaosheng Chen , Jiaqi Li , Ping Gao , Chong Wang , Hao Zhang , Fei Ling , Yanfang Li , Shuihua Xie , Shixin Li and Gongqiu Zhang
  Cathepsin D (CTSD), a major ubiquitously expressed aspartic protease, is not only involved in muscle protein degradation, but also related to some pathological processes. In this study, we characterized the full-length cDNA, genomic DNA sequence, expression profile and polymorphism of the porcine CTSD gene. The full-length cDNA of porcine CTSD gene and the predicted protein sequence shared high identities wih other mammalian orthologous. Northern-blot analysis and Reverse transcription (RT)-PCR results indicated that the CTSD gene has one transcript of approximately 2.0 kb in normal tissues and was expressed ubiquitously in pigs, without significant differences in porcine heart, liver, spleen, lung, kidney, stomach, fat, triceps brachi, biceps femoris, and longissimus muscles. The porcine CTSD gene spans ∼ 9.0 kb including nine exons. All exon/intron boundaries adhere to the GT/AG rule. Altogether 35 nucleotide polymorphisms of CTSD gene were discovered between Duroc, Landrace, Erhualian, and Dahuabai pigs. These polymorphisms included three missense mutations, eight synonymous mutations, and 24 intronic substitutions, and most polymorphisms are located in the intron 4 and 5. Three polymorphisms were genotyped in Duroc, Landrace, Dahuabai, and Erhualian pigs by PCR-RFLP method, and significant differences of their genotype frequencies were observed between Chinese native breeds (Dahuabai and Erhualian) and western breeds (Duroc and Landrace).
  Chong Wang , Gururaj Kulkarni and Huanmin Zhang
  Reproductive characteristics were compared between a series of seven B-congenic lines of White Leghorns retrospectively using eight year’s reproduction records. The records encompass a total of 33,010 fertile eggs set to hatch under controlled conditions, which were collected accumulatively from 2,129 young hens on the USDA-ARS, Avian Disease and Oncology Laboratory specific pathogen free farm during the years (2008-2015). Over the eight year period, average fertility, embryo mortality and hatchability of fertile eggs ranged from 71.6±0.77 (line 15.N-21) to 88.2±0.56 (line 15.P-19), 18.6±0.83 (line 15.6-2) to 28.5±0.84 (line 15.N-21) and 41.2±0.92 (line 15.N-21) to 57.6±1.09 (line 15.6-2) between the B-congenic lines and from 73.0±0.93 (2014) to 87.2±0.58 (2011), 19.5±0.84 (2012) to 35.2±0.97 (2009) and 38.3±1.02 (2009) to 55.4±1.12 (2014) between the years, respectively. Statistical analyses showed both B-congenic line (genetics) and the year of line reproduction (environmental) as well as the line by year (genetics by environment) interaction significantly influenced the reproductive characteristics (p<0.0001). Considerable variation observed between the B-congenic lines each year suggests the reproductive performance followed a polygenic model of inheritance, which are attributable to genes of the major histocompatibility complex (MHC) loci. Findings from this study document the reproductive characteristics of the 7 B-congenic lines of White Leghorns and add to the mounting evidence that MHC affects reproductive characteristics in a diverse species including human, wild chimpanzees, fish, bovine, swine, mice, rats and chickens.
 
 
 
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