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Articles by Chi-Ho Lee
Total Records ( 7 ) for Chi-Ho Lee
  Hyun-Kyung So , P.K. Mandal , O. Baatartsogt , Hee-Kyong Lim , Chi-Ho Lee , Jun-Heon Lee and Kangduk Choi
  To find the alternative for antibiotic this study was carried out to investigate the differentially expressed proteome between Salmonella gallinarum infected and uninfected control in the spleen lymphocytes of ROS broiler chicks fed with Korean mistletoe using proteomic approach. Total four protein spots were detected with differential expression from the chicken spleen lymphocyte in 2DE gels after silver staining. These proteins were characterized by MALDI-TOF MS and MS/MS. Two known proteins were up-regulated viz., Fatty Acid Binding Protein (FABP) and MRP-126 and 2 proteins were down regulated viz., ribosomal protein12, pyruvate kinase. In this experimental fowl typhoid infection in broilers fed with Korean mistletoe through proteomics approach significant differential expression of four proteins were found which appears to be candidate molecules for fowl typhoid.
  Go-Eun Hong , P.K. Mandal , Chang-Won Pyun , K. Choi , Soo-Ki Kim , Kyu-Ho Han , M. Fukushima , Ho-Chul Shin and Chi-Ho Lee
  This study was done to investigate the effects of fermented soy pulp on the osteoporosis in ovariectomized rats. Sprague-Dawley female rats were randomly assigned to four groups as Sham Control (SC), Ovariectomized Control (OC), Ovariectomized and Soy Pulp (OSP) fed and Ovariectomized and Fermented Soy Pulp (OFSP) fed. All rats were fed on purified diets, supplemented with non-fermented and fermented soy pulp on basic diet for 7 weeks. It was observed that isoflavone aglycone was very high in soy pulp fermented for 12 h in comparison to non-fermented soy pulp. Body weight of the rats increased significantly (p<0.05) in comparison to other groups. Atrophy of uterus in OFSP group was significantly (p<0.05) prevented in comparison to OC group. The concentration of estradiol in OFSP group was higher than those of OC and OSP groups. The bone density in OFSP group was significantly (p<0.05) higher than those of OC and OSP groups. The histopathology indicated that OFSP group has better retarded the progress of osteoporosis than other groups. The results showed that isoflavone from the fermented soy pulp has prevented the osteoporosis in ovariectomized rats must be due to its estradiol like function. It is expected that the fermented soy pulp might serve as a functional food in osteoporosis of postmenopausal women.
  Oyungerel Baatartsogt , Prabhat Kumar Mandal , Hee-kyong Lim , Chi-Ho Lee , Jun-heon Lee and Kangduk Choi
  To understand the pathogenesis of PTA we studied the gene expression profiles of synovial cells of knee using gene chip analysis. PTA was induced by transection of the Anterior Cruciate Ligament (ACL) of left knee in 3 piglets. After sacrificing the piglet at 8 weeks, synovial cells were used for gene expression profiling using Affimetrix GeneChip. Affimetrix GeneChip Operating Software and Robust Multi-array Analysis (RMA) were used to identify differentially expressed genes. Expressions of selected genes were verified by RT-PCR. Histopathologic examination at 8 weeks showed overt chronic inflammation indicating the development of PTA. Through genome analyses it was observed that 87 known genes were up-regulated and 76 known genes were down regulated. By analyzing gene expression, we found differential expression of genes related to inflammatory and immune response, lipid binding, cell adhesion, growth activity and muscle development. Some of the important genes related to inflammatory and immune response are FAS, MCP-1, IL-18, IL-1α, IL-6, IL-7, OAS1, TLR4, MX2. RT-PCR result has confirmed the expression of selected genes (IL 6, IL 18, FABP 4, LPL and PLTP) in total RNA isolated from synovial cells. The genome analysis of synovial cells provided us new candidate molecules related to inflammation and immunity which may be useful to understand the pathogenesis of PTA.
  Go-Eun Hong , Prabhat Kumar Mandal , Ki-Won Lim and Chi-Ho Lee
  Isoflavone aglycone content increases after fermentation in soy pulp which is known for prevention of postmenopausal disease. The purpose of this study was to establish optimum fermentation conditions and suitable culture for mass production of aglycone using black soybean pulp, a by-product of soybean processing. Black soybean pulp was fermented up to 48 h using Lactobacillus acidophilus (LA) and Bacillus subtilis (BS) culture. Samples were collected every 12 h to measure isoflavones (daidzin and genistin and daidzein and genistein) using High-performance Liquid Chromatography (HPLC) and β-glucosidase activity using spectrophotometry. Isoflavone aglycone content was verified by Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) analysis. Genistein concentrations in black soybean pulp after 12 and 24 h of fermentation with L. acidophilus were 6.8 and 7.2 fold, respectively, compared to controls. Genistein concentrations in black soybean pulp after 12 and 24 h of fermentation with B. subtilis were 6.8 and 6.9 fold greater than controls. β-glucosidase activity was highest in the black soybean pulp fermented for 12 h with L. acidophilus. Black soybean pulp fermented for 12 h with L. acidophilus was most efficient to produce isoflavone aglycone.
  Eun-Jeong Son , Uttam Kumar Pal , Prabhat Kumar Mandal , Go-Eun Hong , Soo-Ki Kim and Chi-Ho Lee
  This study was performed to determine the hypolipidaemic effects of Allium tuberosum Rottl. (ATR), fermented Allium tuberosum Rottl. (FATR) and processed sulfur. Thirty male Wistar rats were divided into five groups: (i) CON (control, normal diet); (ii) HFD (high fat diet); (iii) HS (HFD+processed sulfur); (iv) AS (HFD+ATR+processed sulfur) and (v) FAS (HFD+FATR+ processed sulfur). Animals were fed the diets for six weeks. Body weights of the animals were recorded in the beginning and end of experiment. The animals were sacrificed, organ and tissue weights recorded and blood and liver samples were analyzed for various biochemical parameters. Body weight gain in the AS group was significantly (p<0.05) lower than the other groups. Spleen and kidney weights in FAS group were significantly (p<0.05) higher than the other groups. The weight of epididymal adipose tissue in AS group was significantly (p<0.05) lower than that of HFD group. The weight of retroperitoneal fat in HFD groups was significantly (p<0.05) higher than other groups. The concentration of aspartate amino transferase (AST) was significantly (p<0.05) higher in HFD group compared to HS, AS and FAS groups. Total liver lipid contents in the HFD group was significantly (p<0.05) higher than the other groups. AS and FAS group rats had significantly (p<0.05) lower triglyceride and total cholesterol compared to HFD group. All together, these results suggest that FATR and processed sulfur may play an important role in reducing body fat deposition and altering the serum and liver lipid profile in rats.
  Go-Eun Hong , Chang-Won Pyun , Sang-Min Jeong , Kyu-Ho Han and Chi-Ho Lee
  This study was performed to determine the effects of fermented Pueraria radix on lipid and bone metabolism in ovariectomized (OVX) rats. Sprague-Dawley rats were divided into 4 groups: A sham operated control group, an OVX control group, an OVX group, treated Pueraria radix and OVX group treated fermented Pueraria radix. Rats were fed experimental diets with Ca and P free for 8 weeks after ovariectomy. The femur weight was significantly increased in the treatment groups, compared with that of the control group. Abdominal fat mass and serum alanine aminotransferase and aspartate aminotransferase activities and serum triglyceride, total cholesterol, LDL-cholesterol and osteocalcin levels in the treatment groups were significantly decreased compared with the control group. Serum estradiol level in the treatment groups was significantly increased compared with the control group. Bone mineral density of the femur in the fermented Pueraria radix treatment groups was significantly increased than the control group. Further, the result of histopathologic observation was shown in that the treatment of Pueraria radix and fermented Pueraria radix prevented trabecular loss. These results suggest that a P. radix powdered supplement as a source of isoflavones might ameliorate serum cholesterol and estradiol levels and protect against bone loss. Furthermore, fermented P. radix might be more effective than non-fermented P. radix and can be useful as a functional food.
  O. Baatartsogt , K. Choi , P.K. Mandal , Hee-kyong Lim , Guan-Hao Li , Hong-Sik Kong , Dae-Hyun Hahm , Chi-Ho Lee and Jun-Heon Lee
  To get insights into pathological pathways of Post Traumatic Arthritis (PTA) this study was done on gene expression profile of synovial cells of knee using gene chip analysis developing experimental post traumatic arthritis (ETA) in swine. ETA was induced by transection of the Anterior Cruciate Ligament (ACL) of left knee in 3 piglets. Articular cartilage and synovial tissues were obtained after 0, 2, 5 and 8 weeks for histopathologic examination. Synovial cells collected after sacrificing the piglet at 8 weeks, were used at 5 passage for gene expression profiling using Affimetrix Gene Chip. Histopathologic examination showed overt chronic inflammation indicating the development of ETA. Through genome analyses it was observed that 87 known genes were up-regulated and 76 known genes were down regulated. By analyzing, it was found that many genes with differential expression are related to inflammation, immune response, lipid binding, cell adhesion, growth activity and muscle development. The present study provided an insight into the TA related gene expression pattern. The genome analysis of synovial cells provided us new candidate molecules which may be useful to understand the pathogenesis of Post Traumatic Arthritis (PTA). The established porcine model may serve as in vivo disease model for further research on traumatic arthritis to elucidate molecular pathogenesis.
 
 
 
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