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Articles by Chetan Sharma
Total Records ( 3 ) for Chetan Sharma
  Chetan Sharma , K.R. Aneja and Ramkrashan Kasera
  In the present study, the antimicrobial and minimum inhibitory concentration of B. aristata leaves and roots extracts were tested by agar well diffusion method against the six ear pathogens namely, Staphylococcus aureus, Proteus mirabilis, Escherchia coli, Pseudomonas aeruginosa, Acinetobacter sp. and Candida albicans. Root extracts displayed antimicrobial activity against all the tested ear pathogens, while leaf extracts showed activity against five tested ear pathogens. The highest antimicrobial activity of B. aristata root extract was found against S.aureus (28.3 mm) in acetonic extract with an MIC of 3.12 mg mL-1 and that of leaf also against S.aureus (27.6 mm) in hot aqueous extract with an MIC of 3.12 mg mL-1. Of the three organic extracts of B. aristata screened, the acetonic extra`ct showed the highest antimicrobial activity. This study suggests that the organic extracts of B. aristata showed broad spectrum antimicrobial activity and may be useful in treating ear infections.
  Smita Chaudhry , Jyoti Luhach , Vandana Sharma and Chetan Sharma
  Application of micro organisms for effective removal of hydrocarbon contamination from soil has been considered by several workers since decontamination of polluted soil by other methods leads to production of toxic compounds and these techniques are non-economic also. In the present study, soil samples from five different petroleum sludge contaminated sites were studied for assessment of their diesel degrading potential. The average heterogeneous fungal count in different soil samples ranged from 35.67±5.69 to 51.33±7.64 and the average count of diesel utilizing fungi ranged from 3.33±1.15 to 26.00±4.00. Total heterogeneous fungal count and diesel utilizing fungal count varied significantly in sludge production and disposal site, as compared to control soil. Thirteen native fungi species of six fungal genera were isolated from different soil samples. The identified fungal genera included Aspergillus, Fusarium, Cladosporium, Penicillium, Rhizopus and Alternaria. Biodegradation ability of all isolates was confirmed by shake flask culture and vapour phase transfer method. The results showed that indigenous fungal isolates Aspergillus sp., Alternaria sp., Penicillium sp. and Fusarium sp. displayed highest capability for biodegradation of diesel. Hence, these fungal species can be used for bioremediation of sludge and diesel contaminated sites.
  Sahaj Kaur , Chetan Sharma , Smita Chaudhry and Robina Aman
  In the present study a comparison on antimicrobial potency of three common weeds i.e. Cannabis sativa, Parthenium hysterophorus and Calotropis procera, were tested by agar well diffusion method against the six common pathogens namely, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Saccharomyces cerevisiae. The in vitro study revealed that organic leaves extract of weeds were more effective than aqueous extract. Among the three tested weeds, methanolic extract of C. sativa was found to be most effective as compare to other weeds. It showed maximum zone of inhibition of 26.3 and 25.6 mm against Bacillus subtilis and Staphylococcus aureus with lowest MIC of 1.56 mg mL‾1. This study revealed that the organic leaves extract of C. sativa showed good antibacterial activity and can be explored for developing antibacterial drugs.
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