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Articles by Chenghua Zhang
Total Records ( 2 ) for Chenghua Zhang
  Xiaoling Ding , Yan Yan , Chenghua Zhang , Yueyun Ding and Zongjun Yin
  In this study, leptin gene was chosen as a candidate gene to evaluate its effect on litter performance traits in four indigenous pig population of Anhui province in China. Two SNPs (U_66254 g.3469 T>C; g. 3714 G>T) in Exon 3 of leptin gene were demonstrated by PCR-SSCP and sequencing of PCR product. Litter performance traits including Numbers of Born Alive (NBA) of the first parity and latter parities were recorded in four pig breeds population. The further association analysis of the 2 SNPs with litter performance traits were conducted in four pig breeds population including 213 sows. Researchers found the 2 SNPs are significantly associated with litter performance traits. The effect of CC genotype of SNP (U_66254 g.3469 T>C) and TT genotype of SNP (U_66254 g.3714 G>T) were significantly higher than other genotypes in both litter traits (p<0.05). The study suggested that lepetin gene could be used for marker assisted selection program as a molecular genetic marker for litter sizes in Chinese indigenous pig breeding.
  Xiaoling Ding , Yueyun Ding , Chenghua Zhang , Long Huang , Xiaolei He and Zongjun Yin
  In order to give a basis for molecular breeding of meet quality traits in Wannan black pig, we applied PCR-RFLP method to analyze the polymorphism of 5'upstream region and intron 2 of H-FABP gene in 184 Wannan black pigs population. The results show that there exist polymorphic loci of restriction enzyme Hinf in 5'upstream region and Hae III as well as Hinf I in intron 2. Except for locus of restriction enzyme HinfI, the other two polymorphism sites achieved in Hardy-Weinberg equilibrium based on χ2-test (p>0.05). In addition, a T→C mutation in 1324 bp, a C→G mutation in 1811 bp and a C→T mutation in 1970 bp were found through the direct sequencing the polymorphic RFLP fragments of H-FABP gene, then genotype frequencies, gene frequencies, Ho, He, Ne and PIC were investigated in Wannan black pig population. The results identified 3 variant restriction sites and provided a basic data for further study of the relationship between IMF and H-FABP with different genotypes and also provide basic for make sure whether H-FABP gene is the key gene for effect on the deposition of IMF.
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