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by
Chen Hao |
Total Records (
2 ) for
Chen Hao |
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Xue Feng
,
Jiang Yuan
,
Xu Fei
,
Zhang Xiao-Rong
,
Luan Jun
,
Zhu Chang-Qing
and
Chen Hao
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In this study, 120 cloacal samples were collected from healthy red-crowned cranes in Yancheng National Rare Bird Reservation District of China and identified by API campy test and multiplex PCR. The results shown that 21.67% (26/120) of these samples were detected to be positive in Campylobacter infection, of which 15 isolates were identified as C. jejuni. Using the transmission electron microscope, we observed the morphologic change of the Bacterial particles with negative staining. C. jejuni showing rod-shape and unipolar sheathed flagella. We found that Campylobacter infection correlated well with age of birds, which was proved by the findings that significant higher isolation rate was obtained in young red-crowned cranes, compared to adult groups. Meanwhile, antibiotics susceptibility test indicated a middle-level of drug resistance was found in these strains. The presence of flaA (100%), cdtB (100%), racR (100%), dnaJ (96.2%), cdtC (96.2%), cadF (95%), pldA (93%), cdtA (93%), ciaB (90%), ceuE (85%), wlaN (40%) and virB11 (0%). Virulence genes was detected in C. jejuni by PCR. The data described here would offer a helpful information for the prevention of Campylobacter infection of wild birds and humans. |
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LI Bi-Chun
,
Tian Zhi-Quan
,
Chen Hao
,
Sun Min
,
Yang Hai-Yan
,
Ren Li-Wei
,
Qin Yu-Rong
,
Xu Feng
and
Chen Guo-Hong
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The effect of Dimethylsulphoxide (DMSO), Glycerol (GLY) and Propanedo (PROH) cryoprotectants, each at (10, 20 and 30%) concentration on the preservation of mouse and cattle skin tissue, was investigated. And then, the toxicity test was performed by exposing the skin tissue to DMSO, GLY, PROH, EG without freezing. Tissues were checked by capacity explants. The results indicated that mouse skin tissue frozen in 10% GLY and cattle ear tissue frozen in 20% GLY medium with dry-ice yielded significantly (p<0.05) higher percents (33.3 and 71.4%) of fibroblast than tissue frozen in either concentration of GLY or cryoprotectants. In the toxicity test, mouse skin tissues exposed to GLY and equilibrated at 4°C resulted in higher percent of average explants (41.1%) than that was exposed to PRON and DMSO (average explants 38.3 and 36.5%, respectively), while cattle tissues showed higher toxicity, when exposed to PROH and EG and equilibrated at 4°C (average explants 39.9 and 44.2%, respectively). The present study recommended that 10% GLY with dry-ice freezing was the effective cryoprotectant for the mouse skin tissues, while 20% GLY with dry-ice freezing was effective for cattle skin tissues. |
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