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Articles by Cheah Yoke Kqueen
Total Records ( 2 ) for Cheah Yoke Kqueen
  Lesley Maurice Bilung , Son Radu , Abdul Rani Bahaman , Raha Abdul Rahim , Suhaimi Napis , Cheah Yoke Kqueen , Chandrika Murugaiah , Yousr Abdul Hadi , Tunung Robin and Mitsuaki Nishibuchi
  Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, this study, demonstrated that the local cockles (Anadara granosa) in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the V. parahaemolyticus strains.
  Nor Asmara Tasrip , Mohd Nasir Mohd Desa , Cheah Yoke Kqueen , Nurul Zarith Mohamad Zin , Zainul Amiruddin Zakaria , Zarizal Suhaili , Chong Pei Pei and Mariana Nor Shamsudin
  This study compared the distribution of aerial mass colors, antibacterial activities and RAPD-based genomic patterns of 39 Streptomyces isolates harvested mainly from soil at the University Agricultural Park, Universiti Putra Malaysia. Streptomyces griseus ATCC 10137 strain was also included as a reference strain (n = 40). Based on ISP-2 media, the aerial mass colors observed were categorized as yellow (n = 15), grey (n = 9), brown (n = 7), white (n = 6) and others (n = 3). Antibacterial activities were assessed on Mueller Hinton Agar (MHA) and Tryptic Soy Agar (TSA) by perpendicular streak method against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Salmonella sp. and Enterococcus sp. MHA demonstrated 15 isolates with broad spectrum antibacterial activities and 18 as non-broad spectrum. TSA gave lower proportions with 15 and 9 isolates respectively. Regardless of the test media used, a higher proportion of isolates with non-white color showed antibacterial activities to suggest a potential correlation. RAPD dendrogram (a composite of 3 random primers) also clustered majority of them but segregated those of white color, which showed less antibacterial activities, in a different cluster. Further validations involving more isolates are warranted to establish the findings.
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