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Articles by Changqing Zhang
Total Records ( 3 ) for Changqing Zhang
  Hancheng Wang , Jin Wang , Wenhong Li , Jiehong Zhao , Maosheng Wang , Ning Lu , Yushuang Guo and Changqing Zhang
  Tobacco black shank caused by Phytophthora nicotianae is one of the most destructive diseases suffered by tobacco in China. Streptomycin, calcium oxide and synthetic fertilizer are three important non-target chemicals used frequently during tobacco growing period. This study has evaluated the activities of the non-target chemicals streptomycin, calcium oxide and synthetic fertilizer on the mycelial growth, sporulation, zoospore formation and germination of cystospores of P. nicotianae. Metabolic fingerprints of P. nicotianae under pressures of those three chemicals are also compared with control treatments. Streptomycin inhibited mycelial growth, sporangia production, zoospore formation and cystospore germination more effectively than the other chemicals tested. Calcium oxide inhibited sporangia production, zoospore formation and cystospore germination at much higher concentration. Mycelial growth of P. nicotianae was not affected by this chemical. Synthetic fertilizer had negative or no effect on sporangia production and no effect on mycelial growth. Zoospore formation and cystospore germination was inhibited at high concentrations of this chemical. Metabolic fingerprint of P. nicotianae was not or poorly affected by calcium oxide and synthetic fertilizer. Streptomycin significantly changed the fingerprint of the pathogen, substrate of y-aminobutyric acid, fumaric acid, L-alaninamide, L-alanyl-glycine, L-proline, L-pyroglutamic acid and putrescine cannot be utilized by P. nicotianae. So it is no use to add calcium oxide to soil for black shank management in the field, while is useful for streptomycin. Synthetic fertilizer in some case may enhance the development of the disease. These new findings provide important information for black shank management in the future.
  Hancheng Wang , Yanfei Huang , Haiqian Xia , Jin Wang , Maosheng Wang , Changqing Zhang and Hongxue Lu
  Tobacco brown spot caused by Alternaria alternata is a devastating disease of tobacco worldwide. Phenotypic characterization of the pathogen was investigated to provide some basic information for biology and pathology by using BIOLOG Phenotype Microarray (PM). Using PM plates 1-10, 950 different growth conditions were tested. Results exhibited that the pathogen was able to metabolize 24.74% of tested carbon sources, 85.26% of nitrogen sources, 97.14% of sulfur sources and 89.83% of phosphorus sources. Most informative utilization patterns for carbon sources of A. alternata were carbohydrates and for nitrogen were various amino acids. The pathogen presented 274 different nitrogen pathways. It had wide range adaptabilities in osmolytes with up to 10% sodium chloride, up to 6% potassium chloride, up to 5% sodium sulfate, up to 20% ethylene glycol, up to 6% sodium formate, up to 6% urea, up to 12% sodium lactate, up to 200 mM sodium phosphate, up to 100 mM ammonium sulfate, up to 100 mM sodium nitrate and up to 20 mM sodium nitrite. It also exhibited active metabolism in the range of pH values between 3.5 and 10, with optimal pH of around 6.0. The pathogen showed active decarboxylase activity, whereas no deaminase activity in the presence of various amino acids.
  Xingjiang Chen , Licui Li , Hancheng Wang , Yanfei Huang , Maosheng Wang and Changqing Zhang
  Background: Tobacco bacterial wilt caused by Ralstonia solanacearum is a devastating disease in Solanaceae crops and the pathogen is a notorious pathogen worldwide. Methodology: The phenotypic characterization of R. solanacearum under various osmolytes and pH environments were analyzed by using biolog Phenotype Microarray system (PMs). Using PM plates 9 and 10, 192 different assays were tested, including 96 different osmolytes and 96 pH environments. Results: Result presented that all four isolates of R. solanacearum exhibited similar phenotypic fingerprints. They had wide range adaptabilities in osmolytes with up to 4% sodium chloride, up to 20% ethylene glycol, up to 4% urea, up to 2% sodium lactate, up to 200 mM sodium phosphate (pH 7), up to 50 mM ammonium sulfate (pH 8), up to 100 mM sodium nitrate and up to 80 mM sodium nitrite. The bacterium did not grow at other osmolytes, including sodium chloride ranging from 5.5-10%, sodium formate ranging from 3-6%, urea ranging from 5-7%, sodium lactate ranging 7-12% and sodium benzoate (pH 5.2) ranging from 100-200 mM. They also exhibited active metabolism under pH values between 5 and 10, with an optimal pH value of around 6. The R. solanacearum showed active deaminase activity, while no decarboxylase activity in the presence of various amino acids. Conclusion: This study increased understanding on the bacterium, especially in survivability of the bacterium in the environment and would provide valuable profiles in developing practical ideas and methods for the disease control.
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