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Articles by Chandrashekhar Singh
Total Records ( 1 ) for Chandrashekhar Singh
  Sunil Kumar Mishra , Bhoosan Yadav , Prabhat Upadhyay , Pradeep Kumar , Chandrashekhar Singh , Jyoti Dixit and Kavindra Nath Tiwari
  Background and Objective: Around 80% people are suffering from central nervous system (CNS) disorder and epilepsy, where most of the patients do not get adequate treatment. Management of epilepsy through synthetic drugs exhibit some side effects and may cause neurological changes. The aim of present study was to examine the Luffa cylindrica entire fruit alcoholic extract for polyphenol profiling, antioxidant and anti epileptic activity followed by biochemical and oxidative stress study and report a safe natural source for the management of epilepsy. Materials and Methods: The prepared extracts were detected in positive and negative mode of UPLC-MS/MS study followed by antioxidant activity through, 1,1-Diphenyl-2-picryl hydrazyl (DPPH) (227.19±0.84), superoxide dismutase (SOD) (2386.03±0.66) and lipid peroxidation (LPO) (226.14±1.38). The same extract was studied for diazepam induced sleep, pentylenetetrazole induced (PTZ) and maximal electroshock (MES, 70 mA) induced convulsion in wistar rat. Oxidative stress and cholinesterase activity of brain tissue was studied by estimating reduced glutathione and lipid peroxidation, total protein in both convulsive models. The changes at cellular level in rat brain histopathology were examined in microscope. Results: The study revealed the presence of total phenol (195.21±3.42 μg mL–1) and flavonoid (641.25±0.24 μg mL–1) and specifically 16 compounds i.e., hyperoside, kaempferol, acacetin, vitexin, leotiolin etc. were identified through LC-MS. The extract (400 mg kg–1) was found potentially active (p<0.05) in all the anticonvulsant study but less than the standard (phenytoin and diazepam). Minimal changes were observed in the extract (400 mg kg–1) and standard drug. Conclusion: Luffa cylindrica is using regularly in daily life as vegetable may be good source of potential antioxidant and anti epileptic activity. The alcoholic entire fruit extract showing the presence of phenolics and flavonoids suppose to be effective for the anti epileptic activity. However, further studies still needed to be carried on the extract for constituent specifically for the reported activity.
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