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Articles by Chaiyasit Sittiwet
Total Records ( 4 ) for Chaiyasit Sittiwet
  Chaiyasit Sittiwet
  The Nelumbo nucifera Gaertn. pollen essential oil was extracted by using vapor distillation. Antimicrobial activity of Nelumbo nucifera Gaertn. pollen essential oil has been investigated using agar diffusion susceptibility test and broth macro-dilution. The essential oil were tested against both gram positive (S. aureus ATCC 25923, S. epidermidis ATCC 12228, M. luteus ATCC 9341, B. subtillis ATCC 6633 and L. plantarum ATCC 14917) and gram negative (E. coli ATCC25922, S. typhimurium ATCC 14028, K. pneumoniae ATCC 10031, P. vulgaris ATCC 13315, Ps. aeruginosa ATCC 9721) bacteria using agar diffusion susceptibility test. Significant zone of inhibition were observed for S. typhimurium ATCC14028 and E. coli ATCC25922). The MICs and MBCs are 10-40 and 20-80 ml L-1, respectively. Nelumbo nucifera pollen essential oil show inhibitory effect on growth of food born pathogen bacteria in low concentration which indicated the possibility of used as food preservation additive.
  Yaowalak Srisuwan , Mangkorn Srisa-ard , Chaiyasit Sittiwet , Yodthong Baimark , Nual- Anong Narkkong and Chirapha Butiman
  Nanocomposite and nanoporous silk fibroin (SF) films were prepared by film casting of SF solution containing surfactant-free colloidal nanoparticles of methoxy poly (ethylene glycol)-b-poly (D, L-lactide) diblock copolymer (MPEG-b-PDLL). Self-condensation and nanophase separation of the nanoparticles from SF film matrix during film drying process gave nanopore structures. The colloidal nanoparticles were prepared in SF solution by modified-spontaneous emulsification solvent diffusion method without any surfactant. The interaction between SF and MPEG-b-PDLL in nanocomposite films was studied by Fourier transform infrared spectroscopy and thermogravimetry. The film transparency of SF nanocomposite films decreased as increasing the MPEG-b-PDLL ratio. Scanning Electron Microscopy (SEM) results indicated that the nanoparticle sizes in the films were in the range of 50-200 nm with spherical shape. Nanopore structures with pore size of less than 150 nm can be observed from SEM images of the film surface and cross-section. The nanopores are interconnected throughout the nanocomposite films. The number and size of nanoparticles and nanopores increased when the MPEG-b-PDLL ratio was increased.
  Chaiyasit Sittiwet
  The P. indica aqueous extract was tested against both gram positive bacteria (S. aureus ATCC 25923, S. epidermidis ATCC 12228, M. luteus ATCC 9341, B. subtillis ATCC 6633 and L. plantarum ATCC 14917) and gram negative (E. coli ATCC25922, S. typhimurium ATCC 14028, K. pneumonia ATCC 10031, P. vulgaris ATCC 13315, Ps. aeruginosa ATCC 9721) using agar diffusion susceptibility test. The result showed zone of inhibition against E. coli and K. pneumoniae. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) are between 1-2 and 4-8 mg L-1 respectively. This result show the possibility of using P. indica as an alternative therapy in the treatment of urinary tract infections.
  Chaiyasit Sittiwet
  Millingtonia hortensis Linn. flowers have been extracted for essential oil using vapor distillation with 0.5-2% yield. The essential oil of M. hortensis Linn. was tested against various species of bacteria. The agar diffusion susceptibility test showed an inhibitory effect  on 6 out of 10 tested strains. The growth of 4 of gram-positive bacteria (S. aureus ATCC 25923, S. epidermidis ATCC12228, B. subtilis ATCC6633 and L. plantarum ATCC14917) and 2 of gram negative bacteria (E. coli ATCC25922 and P. vulgaris ATCC13315) were inhibited by M. hortensis Linn. flower essential oil. The MICs (minimal inhibitory concentration) of M. hortensis Linn. flower essential oil are 0.5-2 and 1-4 ml L-1, respectively. In this study M. hortensis Linn. flower essential oil showed broad spectrum for the anti-microbial activity at low concentration.
 
 
 
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