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Articles by Cece Sumantri
Total Records ( 9 ) for Cece Sumantri
  Niken Ulupi , Muladno , Cece Sumantri and I. Wayan Teguh Wibawan
  Eggs of kampung chicken play an important role as substance in ‘jamu preparation’ in Indonesia, mostly provided and consumed without cooking. Salmonella free eggs become significant in producing the safe ‘jamu preparation’ and such eggs might be produced by chickens which have high resistancy to this bacteria. One of excellent markers showing resistance of chicken against Salmonella is an active Toll-like Receptor 4 (TLR4) gene. TLR4 is a phagocytes cell surface receptor that plays a role to recognize lipopolysaccaride (LPS) of gram negative bacteria including Salmonella enteritidis. It is transcribed by TLR4 gene and conserved in the activation of the non-specific immune system. The aim of the research was to prove how kampung chicken resistant against natural infection of S. enteritidis, using TLR4 gene as marker. TLR4 gene was genotyped in 50 kampung chickens with PCR-RFLP. Then biological assays of resistance indicator were measured. The genotyping result on exon 2 (220 bp in size) identified 3 genotypes of TLR4 gene in kampung chicken: AA, AG and GG. Concentration of leucocytes and their differentiation were not significantly different in AG and GG genotype. The value of it from AA genotype was similar to them. There was no S. enteritidis finding in blood and eggs produced by AA, AG, GG chickens. Specific IgY to S. enteritidis was positively found in kampung chickens serum and egg yolk. Concentration of specific IgY in kampung chicken egg yolk was found very high. The study postulated that most likely kampung chicken resistant to S. enteritidis natural infection.
  Muhammad Amrullah Pagala , Muladno , Cece Sumantri and Sri Murtini
  Tolaki chicken is a kind of Indonesian local chickens, that belong to the ability of anti viral responses. This ability is controlled by the present of antiviral Mx (myxovirus resistance) gene. The Mx gene codes for a protein with antiviral activity. The objective of the study was to prove the tolaki chicken Mx gene genotype is associated with antiviral and production traits. Mx/Hpy 81 gene was genotyped in 103 tolaki chickens with PCR-RFLP. A total of 30 chickens were challenged with ND gen VIIb virus (104 CLD50)/chicken. PCR was used to amplify genomic DNA for Mx gene (299 bp). The amplimer was cut by Hpy 81 produce three genotypes: AA, AG and GG and two alleles: A allele (299 bp) and G allele (200 bp and 99 bp). Frequency of A allele (0.74) was higher than G allele (0.26). The all parameters of production traits in challenge test group were not significantly different in AA, AG and GG genotypes. The daily weight gain, feed intake and FCR were significantly different in AA, AG and GG genotypes of chickens control group. The parameters of antiviral traits showed that vitality of AA (50%) and AG (50%) of chickens were better then GG (10%) in challenge group. The vitality of AA (100%) and AG (100%) were better GG (33.33%) in control group. The study postulated that Mx gene genotype could be associated with production and antiviral traits in tolaki chicken. AA and AG genotype are more resistant and show better production than GG genotype.
  Isyana Khaerunnisa , Muhammad Pramujo , Irma Isnafia Arief , Cahyo Budiman , Asep Gunawan , Jakaria and Cece Sumantri
  Myostatin, or growth and differentiation factor-8 (GDF-8), is a member of the Transforming Growth Factor (TGF)-β superfamily. This family functions as a negative regulator of skeletal muscle. Mutations in exon 2 have been reported to convert Thymine into Guanine (T4842G) that alters the amino acid leucine into arginine, which is associated with body weight in chickens. The objectives of this study were to identify the polymorphism of T4842G mutation in the myostatin gene in Indonesian chickens and evaluate their effects on carcass characteristics. The gene polymorphism was identified with the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using the BsrI restriction enzyme. The effect of genotype on carcass and meat quality was analyzed using the SAS General Linear Model (GLM) procedure. Genotyping was performed on 332 chickens from 7 Indonesian chicken populations (Kampung, Merawang, Sentul, Cobb broiler, F1 crossbreed of Kampung x layer, F1 crossbreed of Kampung x Cobb broiler and F2 crossbreed of Kampung x Cobb broiler). The product of amplification was 247 bp. The myostatin|BsrI locus was polymorphic in all populations, producing two alleles (G and T) and three genotypes (GG, GT, TT). Results from the analysis of the allele and genotype frequency showed that the T allele had a higher frequency than the G allele in all populations, except for the F1 crossbreed of the Kampung x Cobb broiler chicken population, which had equal allele frequencies. A significant effect was found between genotype and carcass characteristics in the F2 crossbreed Kampung x Cobb broiler chickens. A SNP in the coding region of myostatin in exon 2 was associated with live weight, carcass weight, breast weight, thighs weight, drum sticks weight, wings weight, breast muscle weight, thighs muscle weight, drum sticks muscle weight and free water. Here, the association of myostatin|BsrI gene polymorphism with chicken carcass characteristics in Indonesian chickens has been demonstrated, providing evidence that myostatin might be an important candidate gene for chicken carcass characteristics.
  Anggraeni , Asep Gunawan , Rukmiasih , Tuti Suryati and Cece Sumantri
  Background and Objective: Flavin containing monooxygenase 3 (FMO3) is an excellent candidate gene that affects fishy odor and fatty acid composition. It has been reported that down regulation of FMO3 can inhibit fatty acid oxidation. The aim of this study was to investigate the association and expression of the FMO3 gene as a candidate gene for fatty acid composition in Indonesian Cihateup ducks. Methodology: A total of one hundred Indonesian Cihateup ducks were used in this study. Tissues from breast muscles were used for genomic DNA isolation and fatty acid composition analysis. Results: Association analysis showed that the SNP g.849A>G was significantly associated with unsaturated fatty acids (palmitoleic, oleic, linoleic, linolenic and arachidonic acid) and saturated fatty acids (lauric, palmitic and arachidic acid). Compared to the GG genotype, the AG genotype ducks exhibited greater levels (p<0.05) of lauric acid (C14:0), palmitic acid (C16:0), arachidonic acid (C20:4n6) palmitoleic acid (C16:1), oleic acid (C18:1), linolenic acid (C18:3) and linoleic acid (C18:2, p<0.05) but not pentadecanoic acid (C15:0). Furthermore, to analyze the mRNA expression of FMO3 in liver tissues, the ducks were divided into two groups according to the genotypes AG and GG, where AG had relatively favourable unsaturated fatty acid composition. FMO3 mRNA expression was higher (p<0.01) in animals with the AG genotype. Conclusion: These results will improve the understanding of functions of the FMO3 gene in maintaining muscular fatty acid composition and will shed light on FMO3 as a candidate gene in the selection of ducks with unsaturated fatty acids for meat quality improvement.
  Asep Gunawan , Eva Siti Nurajizah , Kasita Listyarini , Ahmad Furqon , Woki Bilyaro , Cece Sumantri , Jakaria , Syeda Hasina Akter and Muhammad Jasim Uddin
  Background and Objective: The stearoyl-CoA desaturase (SCD) gene encodes an enzyme involved in fatty acid (FA) biosynthesis. The aim of the present study was to investigate the association and expression of SCD as a candidate gene for FA composition in Indonesian crossbred chickens. Materials and Methods: Sixty-two Indonesian crossbred, unsexed chickens (F2 Kampung×broilers) were used. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. Results: A single nucleotide polymorphism in coding region c.17492542 C>G of SCD was associated with FA composition, including both unsaturated [linoleic (C18:2n6c) and eicosadienoic (C20:2) acids] and saturated [lauric acid (C12:0)] forms. SCD mRNA expression analysis in liver revealed 6 chickens with extremely high and low FA compositions, of them, high FA birds (n = 3) had higher unsaturated and lower saturated FAs, while the low FA group (n = 3) had lower unsaturated and higher saturated FA levels. SCD expression was higher (p<0.05) in tissues collected from high FA chickens than low FA chickens. Conclusion:These results will improve the understanding of SCD function in FA composition and will shed light on SCD as a potential candidate in the selection of chickens with higher levels of unsaturated and lower levels of saturated FA.
  Asep Gunawan , Anggraeni , Kasita Listyarini and Cece Sumantri
  Background and Objective: The fat mass and obesity-associated gene (FTO) regulates glucose metabolism, body weight and fat content, it is also involved in DNA repair, fatty acid (FA) metabolism and post translational modification. Due to these functions, FTO may affect FAs, carcass and meat quality in ducks. The objective of this study was to identify functional variants of FTO associated with fatness, carcass and meat quality and to investigate the tissue expression profile of the FTO gene in ducks. Methodology: Fifty-seven Indonesian Cihateup ducks were used in this study. Tissues from breast muscle and liver were used to evaluate genomic DNA and mRNA expression. Fatness traits, which include FA composition, carcass and meat quality, were evaluated at 12 weeks. Results: A SNP in exon 3 of the SCD gene was significantly associated with breast muscle for carcass traits and lauric acid (C12:0) for FA composition, however, there was no significant association with meat quality traits. To measure the mRNA expression of FTO, ducks were divide into three genotypes (AA, AG and GG). Compared to the AG and GG genotype, the AA genotype ducks had greater breast muscle weight and higher lauric acid levels (C12:0) for carcass and FA traits, respectively. FTO mRNA expression was significantly higher in genotype AA and GG ducks. Conclusion: The SNP of FTO in exon 3 is a functional SNP that regulates carcass and FAs specific for breast muscle weight and lauric acid levels in ducks.
  Sri Rahayu , Mohamad Yamin , Cece Sumantri and Dewi Apri Astuti
  This study evaluated the effect of a diet containing mung bean sprout waste (MBSW) as a fiber source and feeding time on the growth performance and physiological responses of Garut lambs. Feeding regimens generated by a factorial randomized block design with two factors- diet and feeding time-were assigned to twenty Garut male lambs aged 6-7 months (BW 15.42±2.42 kg). The two diets (D) formulated with a dry matter (DM) base were D1 (60% concentrate 1+40% natural grass) and D2 (60% concentrate 2+40% MBSW). Feeding times were in the morning (MF, 6:00-7:00 am) or the evening (EF, 5:00-6:00 pm). The animals were reared in individual cages and fed with 5% DM/kg body weight/day. Nutrient intake (DM, crude protein (CP), total digestible nutrient (TDN) and average daily gain (ADG) of lambs fed D2 was significantly (p<0.01) higher than that of lambs fed D1. The feed conversion ratio (FCR) of lambs fed with D2 at the EF feeding time was significantly improved (6.07, p<0.05) compared to lambs fed D1 at the MF and EF time, respectively (7.88 and 8.27), or lambs fed D2 at the MF time (7.84). Heart rates (HR) were higher in lambs fed D2 than lambs fed D1. In contrast, the respiration rate (RR) and rectal temperature (RT) were similar for all treatments. These results suggest that diets containing 40% MBSW as a fiber source together with an evening feeding time could increase growth performance while preserving normal physiological responses of Garut lambs.
  Setiawan Putra Syah , Cece Sumantri , Irma Isnafia Arief and Epi Taufik
  Background and Objective: Dangke is a traditional cheese from South Sulawesi that has been developed by the people of the Enrekang district throughout history. The microbiota of this cheese consists of a wide variety of bacterial species. The majority of which belongs to Lactic Acid Bacteria (LAB) genera. The indigenous LAB of dangke could be a potential source of starter cultures and probiotics. The aim of this study was to isolate the LAB from dangke and identify them by 16S rRNA sequencing. Methodology: The dangke from Enrekang, South Sulawesi were collected. The LAB were identified by morphology (Gram staining and cell form), physiology (growth and viability in 6.5% NaCl and temperatures of 15, 37 and 45°C), biochemistry (catalase-negative test and CO2 production) and survival at low pH (2, 3, 4 and 7.2) and in bile salts (0.3%). Results: The results showed that 30 isolates were identified as LAB with Gram-positive, catalase-negative and rod-shaped characteristics. Ten LAB isolates from dangke had highest tolerance to low pH and bile salts. The isolates that were resistant to low pH and bile salts were A123K, A113L, A323L, B111K, B212K, B221L, B312K, B323K, C113L and C222L. The 16S rRNA gene could be amplified by Polymerase Chain Reaction (PCR) from 5 isolates (A323L, B111K, B323K, C113L and C222L) to obtain a single band on a 1% agarose gel. Conclusion: Identification by 16S rRNA gene sequencing showed all isolates were identified as Lactobacillus fermentum with a similarity index of approximately 99-100%.
  Mohammad Sriduresta Soenarno , Cece Sumantri , Epi Taufik , Lilis Nuraida and Irma Isnafia Arief
  Background and Objective: Whey is a byproduct of the cheese industry in Indonesia, while buttermilk is a byproduct from processing butter that has not been used in Indonesia. Whey and buttermilk residues have a very high nutrient value, especially for the growth of microbes. Lactobacillus plantarum (L. plantarum) IIA-1A5 is a lactic acid bacteria that was isolated from Indonesian local beef, Peranakan Ongole (PO). The availability of nutrients in whey and buttermilk provides an opportunity to utilize both as economically valuable growth media specifically for L. plantarum IIA-1A5. The objective of this research was to evaluate the L. plantarum IIA-1A5 fermentation pattern by using different growth media, whey but termilk and whey+skim (whey that was enriched by skimmed milk). Materials and Methods: Lactobacillus plantarum IIA-1A5 was grown in 3 different media: whey buttermilk and whey enriched by skim milk. The bacterial population and pH value were analyzed every 4 h during fermentation. The chemical composition of each media was determined. The best growth media was selected based on the growth rate and generation time of L. plantarum IIA-1A5 during fermentation. Crude antibacterial substances were collected from the best media and analyzed for antimicrobial activity against pathogenic bacteria. Results: Whey and buttermilk had a good nutritive value, including a high amino acid content and allowed complete growth of L. plantarum IIA-1A5. Whey+skim was the best growth medium for L. plantarum IIA-1A5 based on the bacterial generation time, which reached 1.96 h. Lactobacillus plantarum IIA-1A5 produced an antimicrobial substrate during a 20 h fermentation process. The cell-free supernatant, as an antimicrobial substrate, could inhibit pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. Conclusion: The best medium for L. plantarum growth was whey+skim, based on the growth media composition, LAB population, growth rate, generation time and antimicrobial activities.
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