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Articles by C.Y. Liu
Total Records ( 3 ) for C.Y. Liu
  C.Y. Liu , R.L. Zhang , M.X. Chen , J. Li , L. Ai , C.Y. Wu , X.Q. Zhu and R.Q. Lin
  The present study examined sequence variations in the Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) among Angiostrongylus cantonensis isolates from Shenzhen, Qingyuan, Jiangmen and Wenzhou in China. The ITS of nuclear rDNA was amplified from individual A. cantonensis by Polymerase Chain Reaction (PCR) and the representative amplicons were cloned and sequenced. The length of the ITS sequences was 1593-1614 bp for all Chinese A. cantonensis specimens and these sequences were composed of complete ITS-1 sequence of 712-720 bp, complete 5.8 S sequence of 153 bp, complete ITS-2 sequence of 633-650 bp and partial 28 S sequence of 70 bp. The intra-specifc sequence variation in A. cantonensis was 0.1-1.0% for ITS-1 and 0.0-1.3% for ITS-2 whereas sequence comparison revealed that the inter-specifc sequence differences were higher: 15.0-34.6% for ITS-1 and 22.7-24.2% for ITS-2 between A. cantonensis and other Angiostrongylus sp. The results showed that the ITS sequences were conserved among the A. cantonensis isolates however, they were quite different from that of other Angiostrongylus species. Therefore, ITS sequences could provide useful genetic markers for the specific identification and genetic characterization of Angiostrongylus sp.
  Y.G. Ye , R.L. Zhang , Y.R. Zhao , Y.J. Geng , D.N. Huang , S.T. Gao , X.H. Li , C.Y. Liu , X.Q. Zhu and R.Q. Lin
  Anopheles anthropophagus is an important mosquito vector transmitting malaria and Brugia malayi. Due to differences in feeding behaviors between the female and male adult An. anthropophagus, female An. anthropophagus plays a more important role in transmitting pathogens than male ones. The present study identified a gene specifically expressed in female An. anthropophagus from a subtractive cDNA library for female An. anthropophagus and its full-length cDNA was amplified by 3' and 5' Rapid Amplification of cDNA Ends (RACE) technique. This gene was named fsyz and further analyzed by using Northern blot and bio-informatics analyses. It was shown that the fsyz could encode a D7 related salivary protein and was female-specifically expressed and not characterized previously worth further studies.
  B.P. Wang , D. Zhang , Y.Y. Liu , F. Wang , S.Y. Wang , L.D. Han , C.Y. Liu , C.X. Liu , J.P. Liu , J. Pan , W.B. Zhang , Tuo Ya , Zhaori Getu , Daolema , C.H. Huang , J.L. Han , Suya , L.G. Zhang , H.M. Zhou and L. Zhang
  The current study investigated the ovarian response to gonadotropin for establishing a suitable protocol of superovulation in Bactrian camel. Fifteen female camels were randomly divided into 4 groups to compare 4 different superovulation protocols during the natural breeding season. Each camel in 4 groups was injected with FSH at 80, 80, 60, 60, 60, 60, 40 and 40 mg, respectively total dosage of 480 mg, for consecutive 4 days at 12 h intervals. The camels in group 2-4 were naturally mated and subsequently injected 300 IU LH 48 h after the last injection of FSH, the camels in group 1 received the same procedure exception of LH injection. Ovarian follicles and corpora luteas were observed through synchronistic laparotomy 7-9 days after natural mating. The results indicated that there is no significant difference in average ovulation rate (p>0.05) among group 1-3 nor among group 2-4. However, there is considerable difference between group 1 and 4 in average ovulation rate (p<0.05), among which the value is highest (81.38±6.44%) for group 4 but lowest (16.89±7.98%) for group 1. Furthermore, the average number of follicles has yet no obvious difference (p>0.05) among group 1-3 nor among group 2-4 but significant difference (p<0.05) between group 1 and 4. Comparatively the animals in group 1 yielded highest average number of follicles (9.80±1.50). Conclusively, the protocol 4 had a best superovulatory effect with an average corpora lutea 9.33±1.45 and therefore it can be used for superovulation in camels.
 
 
 
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