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Articles by C.N. Ariole
Total Records ( 2 ) for C.N. Ariole
  N.G. Anyanwu and C.N. Ariole
  Background and Objective: Antibiotic resistance problem has led to the search for alternate approaches for disease management in aquaculture systems. Probiotic potential of indigenous marine Bacillus thuringiensis was carried out on shrimp (Penaeus monodon) culture infected with Vibrio mimicus in a non-flow through system. Materials and Methods: Bacillus species (potential probiotic candidates) were isolated from healthy shrimp intestine while pathogenic Vibrio mimicus was previously isolated from moribund shrimp intestine. Agar well diffusion assay was employed for in vitro antibacterial assay. Acute pathogenicity test followed by histopathological examination of shrimp hepatopancreas were carried out. Challenge test was carried out with Vibrio mimicus for 12 days after addition of the antagonistic bacterium. Water quality parameters such as temperature, pH, salinity, ammonia, nitrite, nitrate and dissolved oxygen were monitored. Results: Bacillus sp., B1 amongst the investigated potential probiotic candidates proved antagonistic to Vibrio mimicus with inhibition zone of 16.0±0.32 mm. Histopathology result proved safety of Bacillus sp., B1 when it neither cause any mortality on the test shrimp, nor cause any detrimental effects on their internal organs while Vibrio mimicus proved lethal to the postlarvae. Molecular identification of Bacillus sp., B1 revealed its close relatedness to Bacillus thuringiensis strain G5-8-3T02. Conclusion: Challenge test revealed that shrimp with the probiotic addition exhibited resistance to the pathogen. This was expressed by the weight and length increase in the experimental shrimp. Water quality parameters were also at optimum levels throughout the culture period. Indigenous Bacillus thuringiensis strain G5-8-3T02 could be a good probiotic bacterium for shrimp culture against vibriosis.
  C.N. Ariole , A.A. Onuorah and H.O. Stanley
  Background and Objective: Siderophores are essential in the extremely iron-reduced environment of the intestine where probiotic bacteria effectively compete for iron. The diversity and antibacterial potential of siderophore producing bacteria isolated from marine fish species from the bight of Bonny at Bonny Island, Nigeria were investigated. Materials and Methods: Bacteria were isolated from intestine of 10 marine fish species: Sleeper (Eleotridae bostrychus), Smooth grouper (Dermatolepsis striolata), Angel fish (Monodactylus argenteus), Tilapia (Tilapia guiniensis), Silver catfish (Chrysichthys auratus), Snapper (Lutjanus gorensis), Grunt (Pomadaysis jubilenie), Barracuda (Kudoa sphyraeni), Mud skipper (Periophthalmus papillo) and Mullet (Mugil cephalus) using spread plate technique. Isolates were screened for siderophore production using Chrome Azurol Sulphonate (CAS) assay and percent siderophore units were determined. Siderophore producers were screened for antibacterial activity against 5 marine pathogens (Escherichia coli, Salmonella sp., Staphylococcus aureus, Vibrio cholerae and Vibrio paraheamolyticus) using agar well diffusion assay. The active strains were identified using molecular method. Results: Seventy four bacterial strains belonging to 13 genera (Pseudomonas, Staphylococcus, Salmonella, Shigella, Vibrio, Bacillus, Klebsiella, Escherichia, Aeromonas, Proteus, Providencia, Morganella and Enterobacter) were isolated from the samples at different frequencies with Staphylococcus and Vibrio predominating. Seven fish species harboured at least one siderophore producer. All siderophore producing isolates inhibited at least one pathogen. Six marine fish species that harboured 10 bacterial isolates belonging to 7 genera which produced siderophore with strong antibacterial potential are Angel fish: Pseudomonas aeruginosa strain ET05, Aeromonas sp. Strain JDMASP8, Morganella morganii strain HX08027 and Vibrio fluvialis strain FSP561/08; Sleeper fish: Vibrio fluvialis strain ATCC33809 and Morganella morganii strain FDAARGOS 172; Smooth grouper: Enterobacter aerogenes A244438.1; Silver fish: Proteus sp. SBP10; Snapper: Providencia stuartii strain S2S and Grunt fish: Aeromonas caviae strain AH07. Conclusion: These organisms and their siderophores can be characterized for use as siderophore genic probiotics for iron nutrition and biocontrol in marine aquaculture.
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