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Articles by C.C. Sieo
Total Records ( 2 ) for C.C. Sieo
  C.C. Sieo , N. Abdullah , W.S. Tan and Y.W. Ho
  Ten Lactobacillus strains (L. crispatus I12, L. brevis I23, I211, I218 and C10C, L. fermentum I25, I24C and C17C and L. acidophilus I16C and I26C) which originated from the gastrointestinal tract of chickens were transformed with plasmids pSA3b3 and pSA3b6. These plasmids harbored a bglA gene, cloned in opposite orientation, which encoded for β-glucanase enzyme. Enzyme expression was only detected in pSA3b6 transformants. The plasmid stability in transformants L. crispatus I12pSA3b6, L. brevis I23pSA3b6, I211pSA3b6, I218 pSA3b6 and C10CpSA3b6 and L. fermentum I25pSA3b6, were comparatively higher than the other strains. Approximately 30-48% of the cells retained the plasmid after 21-36 generations of growth. All the strains, except for L. brevis C10CpSA3b6, produced 22.8-25.1 U mL-1 of β-glucanase during late exponential growth phase (14 h old cultures). In L. brevis C10CpSA3b6, 37.2 U mL-1 of β-glucanase was detected during early exponential phase (6 h old culture).
  C.C. Sieo , B.T. Wong , N. Abdullah and Y.W. Ho
  Whole-cell protein fingerprinting has been used in various studies for identification, characterization and differentiation of bacteria. It has been observed that the methods of extraction of the whole-cell protein and age of the cells used were varied in several studies. Thus, in this study, the effects of different extraction methods and age of cells on the whole-cell protein patterns were evaluated. The whole-cell proteins from Lactobacillus reuteri C1, C10 and C16, Lactobacillus salivarius I24 and Lactobacillus gallinarum I16 and I26 were extracted from 6, 12, 18 and 24-h-old cells by using lysozyme treatment, glass beads and sonication. A comparison of the whole-cell protein profiles generated showed that extraction by lysozyme treatment produced the most complete profile, with protein bands at high intensities. Glass beads and sonication could only extract major proteins. The age of cells also affected the whole-cell protein profile. Different strains were found to produce optimum results at different cell ages when extracted with the three methods.
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