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Articles by C.-X. YE
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  C.-X. YE , L.-X. TIAN , H.-J. YANG , J.-J. LIANG , J. NIU and Y.-J. LIU
  This study was conducted to investigate the effect of dietary manganese (Mn) on growth, vertebrae and whole-body Mn content of juvenile grouper, and to examine the effect of dietary Mn on copper (Cu), iron (Fe), zinc (Zn), calcium (Ca), phosphorus (P) and magnesium (Mg) content of vertebrae and whole body. Seven casein-gelatin-based diets were supplemented with 0, 5, 10, 15, 20, 50 and 1000mgkg-1 of Mn from MnSO4·H2O. Grouper with an initial weight of 12.9±0.4g were fed to satiation with one of the seven diets for 8weeks. Growth was not significantly affected by dietary Mn supplements. Vertebrae Mn increased from 31.7 to 118.1mgkg-1 dry weight with dietary Mn supplement increasing from 0 to 50mgkg-1 (y= -0.0002x3+ 0.0162x2+1.3903x+ 26.27, R2= 0.9561, where y is the vertebrae Mn content and x is the dietary Mn content). Whole-body Mn increased from 2.5 to 7.8mgkg-1 wet weight with dietary Mn supplement increasing from 0 to 50mgkg-1 (y=0.00001x3- 0.00107x2+0.11054x+ 2.24615, R2=0.9080, where y is the whole-body Mn content and x is the dietary Mn content). Dietary Mn had no significant effect on vertebrae Fe, Ca, P and Mg content, and whole-body Cu, Zn and Mg content. However, vertebrae Zn and whole body Ca, P were highest in fish fed diet supplemented with 15mgkg-1 of Mn. Based on this, Mn supplement of 15mgkg-1 might be the optimum when the basal diet contained 4mgkg-1 of Mn. Fish fed diet supplemented with 1000mgkg-1 of Mn did not show any gross abnormality or change in feeding behaviour, but Mn contents of vertebrae and whole body were as high as 695.1mgkg-1 dry weight and 42.5mgkg-1 wet weight, respectively. Also, whole body Fe decreased significantly when Mn supplement was up to 1000mgkg-1.
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