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Articles by C. Latha
Total Records ( 2 ) for C. Latha
  E. Nanu , C. Latha , B. Sunil , Prejit , Magna Thomas and K. Vrinda Menon
  In order to get an insight on the microbial quality assurance of milk at the point of production (farmer’s level), a total of 240 raw milk samples were collected from farmers belonging to three farmer dairy societies of Kerala, viz; FS1, FS2 and FS3 and were examined to estimate the microbial load per ml of milk and also to detect the presence of bacterial pathogens of public health significance. The quality of each samples were evaluated by estimating the Total Viable Count (TVC), Coliform Count (CC), Psychrotrophic Count (PC), Faecal Streptococcal Count (FSC) and Yeast and Mould Count (YMC). The samples collected from the farmers belonging to society 2 (FS2) had the highest microbial load with respect to the TVC, CC, PC and YMC with highly significant (p≤0.01) difference between mean TVC, CC and PC of the samples obtained from the FS1 and significant (p≤0.05) difference between mean YMC of FS. The samples belonging to FS3 has the highest mean FSC. Among the pathogenic isolates, E. coli was detected in 76 (31.6%) samples and the isolates consisted of the serotypes 05, 024, 025, 068, 084, 087, 0103, 0116, 0125, 0145, 0157 and 0172. Stap. aureus was isolated from 84 samples (35%), Yersinia from 118 and L. monocytogenes from only one sample. Among spoilage causing organisms viz., Aeromonas, Pseudomonas aeroginosa, Lactobacillus and Proteus, the isolates obtained were 88, 10.8, 83.7 and 5%, respectively. In order to identify the Critical Control Points (CCP) of microbial contamination of milk the samples of air, water, hand wash, udder wash and utensil rinsing obtained from the farmers were evaluated and observed high counts from samples of udder and hand wash indicative of milk contamination.
  Prejit , E. Nanu and C. Latha
  The microbial quality assurance of milk was done by evaluating 200 samples of milk from production point (dairy farm = 60), processing plant (60) and from market (80). Out of 60 samples of raw milk analyzed, 20 each from individual animal after complete milking (IAM), Pooled milk (PM) and chilled milk (CM), the PM revealed maximum contamination with highly significant (p≤0.01) difference between mean Total viable count (TVC) and Coliform count (CC) and significant (p≤0.05) difference between mean Psychrotrophic count (PC) and Faecal Streptoccal count (FSC) of milk from IAM. From the sample of milk (20 each) collected from pasteurization plant viz. immediately after heating (AHM), after pasteurization (Pasteurized milk or PAM) and after packaging (Packaged milk or PGM) the mean TVC was high in PGM. Only 40% of PGM was graded satisfactory based on coliform standards prescribed by BIS. On comparison of PGM obtained from dairy plant with the retail brands revealed that PGM had highly significant (p≤0.01) difference and lesser TVC, CC and PC in comparison with the brands B, C and D. The samples of the brand B had highest mean TVC. The highest mean CC, ECC, PC and FSC were seen in the samples of brand C. Evaluation of various critical points of bacterial contamination of milk from production and processing sites revealed high microbial count from milk pail, milkers hand washings and package machine wash indicating an important sources of contamination.
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