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Articles by C. Bounphanousay
Total Records ( 2 ) for C. Bounphanousay
  C. Bounphanousay , P. Jaisil , J. Sanitchon , M. Fitzgerald , N.R. Sackville Hamilton and J. Sanitchon
  Fifty three accessions of lowland Black Glutinous Rice (BGR) and three white rice varieties from Lao PDR were evaluated for variations in 2-acetyl-1-pyrroline (2-AP), a main aroma compound present in fragrant rice and a 257 bp genomic fragment associated with a gene encoding 2-AP. The objective of this study was to characterize the variation in aromatic characters of Lao BGR by using chemical and molecular techniques. Thirty six out of the 56 accessions had aromatic properties, containing 2-acetyl-1-pyrroline (2-AP). The concentrations of 2-AP in unpolished samples were about 50% higher than those of the polished samples. The concentrations of 2-AP in BGR accessions were lower than those of white aromatic rice varieties in which 2-AP was highest in Kai Noi Leuang (0.688 ppm). Seventeen accessions and two of white rice (Kai Noi Leuang and TDK5) had a common 355 bp allele corresponding to that found in Calrose, a non-fragrant variety. A 257 bp allele was found in the 36 varieties of BGR and a local aromatic white rice variety, Hom Nang Nouan, sharing a common allele as in the fragrant variety KDML 105. Surprisingly, Kai Noi Leuang, a popular Lao local aromatic rice variety with the highest 2-AP concentration did not possess a 257 bp fragment which has been identified previously as a fragrant allele. This is the first study of the chemical and molecular aromatic properties of lowland BGR varieties of the Lao PDR and the data obtained will be valuable in screening other germplasm and in planning a breeding program in the country geared towards fragrant rices with superior agronomic properties.
  C. Bounphanousay , P. Jaisil , K.L. McNally , J. Sanitchon and N.R. Sackville Hamilton
  The genetic diversity of 74 genotypes, including Black Glutinous Rice (BGR) from Lao`s germplasm was assessed using 24 microsatellite markers. A total of 75 alleles were detected at the 24 microsatellite markers. The number of alleles per marker varied from 2 to 7 with an average of 3.1 alleles per locus. The Gene Diversity (GD) and Polymorphism Information Content (PIC) ranged from 0.18 to 0.79 and 0.17-0.76, respectively and the Allele Frequency (AF) ranged from 0.36 to 0.90. The markers were able to classify rice genotypes into four groups; indica rices were put in the three groups while the other group consisted of tropical japonica rice. The first indica group (G1) included 24 genotypes of BGR and five genotypes of white rice. Most of genotypes in this group have thick culms, broad leaf blades, large and bold grain shapes and some of them have purple coloration on all vegetative parts. In other varieties with black pericarp, all other plant parts are green. Indica group 3 (G3) included 25 genotypes of BGR. The special characters of this subgroup were small and slender culms, narrow short leaves, purple leaf margins, purple leaf tips and purple stripes on leaf blades and sheathes. However, the markers used could not differentiate between LG 8215 and LG 7937. Indica group four (G4) consisted of eight genotypes of white rice and the four check varieties. Group (G2) consisted of five white rices, four BGRs and three check varieties.
 
 
 
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