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Articles by C. Udayasoorian
Total Records ( 6 ) for C. Udayasoorian
  P.C. Prabu and C. Udayasoorian
  The white rot fungus Phanerochaete chrysosporium isolated from paper mill effluent enriched soil samples degrading various phenol (Mono, di hydroxy and methoxy) compounds. During five days of incubation period, 74% of para-hydroxy benzoic acid was utilized by the isolated Phanerochaete chrysosporium when glucose used as a co-substrate. There was 57% degradation of protocatechuic acid using fructose. The fungus degraded vanillin in the presence of co-substrates and the response was more in glucose and fructose than starch. The presence of laccase (EC.1.10.3.2) and polyphenol oxidase (EC.1.10.3.0) extracellular activity suggested that the microorganism secrete these enzymes into the extracellular medium.
  P.C. Prabu and C. Udayasoorian
  A white rot fungus isolated from soil samples enriched by continuous pulp and paper mill effluent irrigation and identified as Phanerochaete chrysosporium was capable of decolorization and degradation of chlorinated phenol from paper mill effluent. There was 84% effluent decolourisation along with 79% COD reduction by Phanerochaete chrysosporium. The effluent chlorinated phenol degradation was 91% by the fungus when added with 1% glucose as co-substrate.
  P.C. Prabu , C. Udayasoorian and G. Balasubramanian
  A new lignin degrading basidiomycete, Phanerochaete chrysosporium (TL 1) was isolated from pulp and paper mill effluent enriched soil samples can be induced to produce high level laccases when grown on a cellobiose-asparagine liquid medium with 150 μM CuSO4. The fungus grown under static conditions had 70% of total extra cellular laccase protein and about 2.5 fold purification with a final yield of 13.2% of protein purification by Sephadex G-100 column and FPLC. The resultant enzyme pool of the purification process is found to contain a single polypeptide, which produced a single band on an SDS-PAGE. The purified protein showed a specific activity of 106 U mg-1 and the molecular mass (Mr) of native laccase was 65 kDa. The purified laccase has an isoelctric point of 4.0, it is stable in pH range from 4.0 to 6.0 and its optimum pH is 4.5. The optimal reaction temperature is 60°C and stable at 70°C for more than 1 h. Degenerative primers corresponding to the consensus sequences of the copper binding regions in the N-terminal domains of known basidiomycete laccase were used to isolate laccase gene specific sequences from this strain and the laccase gene gave PCR product of about 150 bp and cloned product gave 85% similarity with laccase from T. villosa LCC 2 (L49377).
  C. Udayasoorian , P.C. Prabu and G. Balasubramanian
  The white rot fungus isolated from continuous pulp and paper mill effluent irrigated soil and identified as Phanerochaete chrysosporium (TL 1) was capable of degrading pentachlorophenol. 14C synthetic lignin mineralization assays showed that the fungus assimilated 33.6% of the total label. Removal and degradation of pentachlorophenol (PCP) by the organism in static flask cultures was studied using ammonium lignosulphonate (LS), a waste product of paper mill industry, as a carbon and nitrogen source. After eight days, Phanerochaete chrysosporium (TL 1) grown in 2% LS (nitrogen sufficient) medium removed 85% of PCP which was comparable that of degradation in 2% glucose medium (93%). The presence of laccase (EC.1.10.3.2) extracellular activity suggested that the fungus secrete the enzyme into the extracellular medium, which was responsible for the degradation on PCP. The extracellular laccase activity was assayed on agarose plates containing ABTS.
  P.C. Prabu and C. Udayasoorian
  A white rot fungus isolated from soil samples enriched by continuous pulp and paper mill effluent irrigation and identified as Trametes versicolor was capable of decolorization and degradation of phenol from paper mill effluent. 14C synthetic lignin mineralization assays showed that Trametes versicolor assimilated 24.3% of the total label. There was 76% effluent decolourization along with 78% COD reduction. The effluent chlorinated phenol degradation was 85% by Trametes versicolor, when added with 1% glucose as co-substrate.
  C. Udayasoorian and P.C. Prabu
  The white rot fungus isolated from paper mill effluent enriched soil samples and identified as Trametes versicolor was capable of degrading phenol (Mono, di hydroxy and methoxy) compounds.14C synthetic lignin mineralization assays showed that Trametes versicolor assimilated 24.3% of the total label. During five days of incubation period, 71% of para-hydroxy benzoic acid was utilized by Trametes versicolor when glucose used as a co-substrate and 56% degradation of protocatechuic acid was achieved using fructose. The presence of laccase (EC.1.10.3.2) and polyphenol oxidase (EC.1.10.3.0) extracellular activity suggested that the fungus secrete these enzymes into the extracellular medium and the extracellular laccase activity was assayed on agarose plates containing ABTS.
 
 
 
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