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Articles by C. Shiva
Total Records ( 3 ) for C. Shiva
  F. Figueras , C. Adelantado , C. Shiva , J.J. Hernandez and M.A. Calvo
  This study provides Enterobacter spp. lactose negative strains characterization susceptible of being wrongly identified as Salmonella spp. having into account its meaning for what concerns on food safety. More than five hundred feed samples for farm animal feeding were microbiologically analyzed according the Spanish Normative for feed, in which different media are accepted for detecting Salmonella spp. after performing preenrichement steps. In this study SS agar was chosen for differential isolation. Results show that 55% of those colonies supposed to be Salmonella spp. belonged to the genus Enterobacter when performing confirmation by means of API20E method and TSI/LIA tests, indicating that several strains of the genus Enterobacter have lost their condition of lactose positive microorganisms and becoming lactose negative, inducing confusion when trying to identify Salmonella spp. by traditional and reference methods.
  M.A. Calvo , E. Angulo , P. Costa-Batllori , C. Shiva , C. Adelantado and A. Vicente
  Two trials were carried out to study how a compound formed by natural extracts from Rutaceae plants, cinnamon oil and organic acids (RUTEX® PG) could have an effect on intestinal microorganisms on piglets. To obtain this synergistically combination first of all natural extracts and organic acids were separated individually to see its inhibitory action on bacterial growth in vitro. Secondly, the aim was to search the best natural extract and organic acid combination between all the possible ones. RUTEX® PG was the combination that was used in vivo trials with piglets. To study RUTEX® PG effects on piglets two trials were designed. The trials consisted in a comparison between the effect of adding RUTEX® PG to the feed on the intestinal microorganisms of piglets and the effect of antibiotics in feed. The in vitro experiments results showed a synergistic effect of the Rutaceae plant extract and the organic acids tested (citric, formic, lactic and propionic) against different microorganisms: growth inhibition was observed for the organic acid-natural extract combination, while the same concentrations did not show any inhibition when the products were tested separately. Inhibitory activity for cinnamon oil was not tested, because it was added in a very low concentration just to confer an aromatic effect. It was observed, as well, lower bacterial counts at the intestinal segments (duodenum, jejunum, ileum and colon) in comparison with the negative control group. The conclusion is that we can say RUTEX® PG can be active all along the gastro-intestinal segments.
  C. Adelantado , C. Shiva , L. Arosemena , P. Costa-Batllori and M.A. Calvo
  Rutaceae extracts enzymatic activities were studied in order to find out their possible relation with the antimicrobial activity that they possess as well as the improvement of other productive aspects. Several enzymatic activities were detected (Acid phosphatase, alkaline phosphatase, esterase, lipase, leucine arylamidase, trypsine, valine arylamidase, cystine arylamidase, α-fhymotrypsine, naphtol-AS-BI-phosphohydrolase, α-fucosidase, α and β galactosidase, β-glucuronidase, α and β glucosidase, α-mannosidase and N-acetyl-β-glucosaminidase) and special attention is paid to those enzymes that have direct effect on either the bacterial cell structure or the bacterial metabolism.
 
 
 
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