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Articles by C. Nwinyi Obinna
Total Records ( 2 ) for C. Nwinyi Obinna
  C. Nwinyi Obinna and Nduchukwuka Destiny
  Background and Objective: There has been an unusual increase in the emergence of antimicrobial resistance to most of the commonly used antibiotics particularly in the developing countries. Materials and Methods: In this study, five bacteria species tentatively named NC1-5 were isolated from these vegetables: Corchorus olitorius (L.), Celosia caracas, Vernonia amydalina and Abelmoschus esculentus and the irrigation water in Ado-Odo Ota, Nigeria. The isolates were characterized culturally, morphologically and biochemically. The organisms were compared with standard reference organisms and were proposed to be species of Escherichia coli, Chromobacterium violaceum, Micrococcus luteus, Shigella and Salmonella. These bacteria species were tested for their susceptibility to standard Gram positive and Gram negative antibiotics by agar disk diffusion method. The tested antibiotics at different concentrations include: cotrimazole, cloxacillin, erythromycin, gentamycin, augmentin, streptomycin, tetracycline and chloramphenicol. Results: Chromobacterium violaceum exhibited 100% susceptibility to all antibiotics tested, while other bacteria species showed different susceptibility patterns ranging from susceptible, intermediate resistant and resistant patterns. Conclusion: From this study, vegetables can be an avenue for transferring antibiotic resistant genes in community settings. Hence it is necessary to monitor and understand the critical points in the control of antibiotic resistance among the developing countries.
  C. Nwinyi Obinna , Nwodo-Chinedu Shalom and O. Amund Olukayode
  Two closely related strains of Rhodococcus species, isolated from aniline polluted tropical ecosystem, were able to utilize aniline as carbon source in 3.0 and 4.0 mM concentrations at 30°C and pH of about 6.4. Rapid increase in turbidity and a sharp decline in pH were observed in the cultures of both organisms within 24 h of incubation. Shortly after the period, growth became slower. Turbidity values obtained at 4.0 mM concentrations of aniline was about twice the values obtained at 3.0 mM concentrations. Aniline concentrations of 10 mM and above were found to be toxic for the organisms. Rhodococcus species because of its significant prevalence in agricultural soils can be used as an effective means of recovering tropical agricultural land polluted with aniline, aniline-based herbicides or its derivatives.
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