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Articles by C. C Hsu
Total Records ( 2 ) for C. C Hsu
  H. L Huang , F. L Chen , C. C Hsu , Y. Y Yen , T Chen , C. M Huang , H. Y Shi , C. Y Hu and C. H. Lee

The aim was to comprehensively examine school-based tobacco policy status, implementation and students’ perceived smoking at school in regard to gender-specific differences in smoking behavior. We conducted a multilevel-based study to assess two-level effects for smoking among 2350 grades three to six students in 26 randomly selected elementary schools in southern Taiwan. A series of multilevel models were analyzed separately for male and female students. The school-level variables appear to be related to smoking behavior in male students. Among males, the risk of ever-smoking was significantly associated with those schools without antitobacco health education activities or curricula [adjusted odds ratio (aOR) = 6.23, 95% confidence interval (CI): 2.55–15.24], with a high perceived smoking rate (aOR = 3.08, 95% CI: 1.41–6.72) and located in a mountainous region (aOR = 2.53, 95% CI: 1.15–5.58). The risk of ever-smoking among females was significantly associated with those schools without antitobacco activities or curricula (aOR = 3.10, 95% CI: 1.27–7.55). As compared with female counterparts, the specific school that the male students attended had a positive significant effect on the risk of being ever-smokers. The findings suggest that effective tobacco policy implementation should be considered in elementary schools that are currently putting children at the greatest risk for cigarette smoking, especially in regard to male students.

  C. C Hsu , W. C Tsai , C. P. C Chen , Y. M Lu and J. S. Wang

Negative-pressure wound therapy has recently gained popularity in chronic wound care. This study attempted to explore effects of different negative pressures on epithelial migration in the wound-healing process. The electric cell-substrate impedance sensing (ECIS) technique was used to create a 5 x 10–4 cm2 wound in the Madin-Darby canine kidney (MDCK) and human keratinocyte (HaCaT) cells. The wounded cells were cultured in a negative pressure incubator at ambient pressure (AP) and negative pressures of 75 mmHg (NP75), 125 mmHg (NP125), and 175 mmHg (NP175). The effective time (ET), complete wound healing time (Tmax), healing rate (Rheal), cell diameter, and wound area over time at different pressures were evaluated. Traditional wound-healing assays were prepared for fluorescent staining of cells viability, cell junction proteins, including ZO-1 and E-cadherin, and actins. Amount of cell junction proteins at AP and NP125 was also quantified. In MDCK cells, the ET (1.25 ± 0.27 h), Tmax (1.76 ± 0.32 h), and Rheal (2.94 ± 0.62 x 10–4 cm2/h) at NP125 were significantly (P < 0.01) different from those at three other pressure conditions. In HaCaT cells, the Tmax (7.34 ± 0.29 h) and Rheal (6.82 ± 0.26 x 10–5 cm2/h) at NP125 were significantly (P < 0.01) different from those at NP75. Prominent cell migration features were identified in cells at the specific negative pressure. Cell migration activities at different pressures can be documented with the real-time wound-healing measurement system. Negative pressure of 125 mmHg can help disassemble the cell junction to enhance epithelial migration and subsequently result in quick wound closure.

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