Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by C. A Dodson
Total Records ( 1 ) for C. A Dodson
  C. A Dodson , N Ferguson , T. J Rutherford , C. M Johnson and A. R. Fersht
 

The SAP domain from the Saccharomyces cerevisiae THO1 protein contains a hydrophobic core and just two -helices. It could provide a system for studying protein folding that bridges the gap between studies on isolated helices and those on larger protein domains. We have engineered the SAP domain for protein folding studies by inserting a tryptophan residue into the hydrophobic core (L31W) and solved its structure. The helical regions had a backbone root mean-squared deviation of 0.9 Å from those of wild type. The mutation L31W destabilised wild type by 0.8 ± 0.1 kcal mol–1. The mutant folded in a reversible, apparent two-state manner with a microscopic folding rate constant of around 3700 s–1 and is suitable for extended studies of folding.

 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility