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Articles by C Zhao
Total Records ( 5 ) for C Zhao
  Z Liu , Z Yu , N Liu , C Zhao , J Hu and Q. Dai
 

In our efforts for cloning novel I2-superfamily conotoxins using the signal peptide sequence, we identified a novel conotoxin Lt12.4 from Conus litteratus. This gene has a framework XII (-C-C-C-C-CC-C-C-), which is distinct from the cysteine pattern I2-superfamily conotoxin (-C-C-CC-CC-C-C-). Subsequently, we found the signal peptide sequence of Lt12.4 by 5'-RACE. Using this new sequence, we identified another five novel conotoxins with this cysteine pattern from four Conus species (Conus eburneus, Conus imperialis, Conus marmoreus, and C. litteratus). These novel conotoxins have the same cysteine pattern as the reported Gla-TxX and Gla-MII, and may contain Gla residues. Furthermore, they have the highly conserved signal peptide and hypervariable mature peptide sequences, and widely exist in Conus species. Therefore, it could be defined as a new superfamily of E-conotoxins.

  C Zhao , I Ivanov , E. R Dougherty , T. J Hartman , E Lanza , G Bobe , N. H Colburn , J. R Lupton , L. A Davidson and R. S. Chapkin
 

We have developed novel molecular methods using a stool sample, which contains intact sloughed colon cells, to quantify colonic gene expression profiles. In this study, our goal was to identify diagnostic gene sets (combinations) for the noninvasive classification of different phenotypes. For this purpose, the effects of a legume-enriched, low glycemic index, high fermentable fiber diet was evaluated in subjects with four possible combinations of risk factors, including insulin resistance and a history of adenomatous polyps. In a randomized crossover design controlled feeding study, each participant (a total of 23; 5–12 per group) consumed the experimental diet (1.5 cups of cooked dry beans) and a control diet (isocaloric average American diet) for 4 weeks with a 3-week washout period between diets. Using prior biological knowledge, the complexity of feature selection was reduced to perform an exhaustive search on all allowable feature (gene) sets of size 3, and among these, 27 had (unbiased) error estimates of 0.15 or less. Linear discriminant analysis was successfully used to identify the best single genes and two- to three-gene combinations for distinguishing subjects with insulin resistance, a history of polyps, or exposure to a chemoprotective legume-rich diet. These results support our premise that gene products (RNA) isolated from stool have diagnostic value in terms of assessing colon cancer risk.

  Z Zhao , C Zhao , X. H Zhang , F Zheng , W Cai , H Vlassara and Z. A. Ma
 

Advanced glycation end products (AGEs) are implicated in diabetic complications. However, their role in β-cell dysfunction is less clear. In this study we examined the effects of AGEs on islet function in mice and in isolated islets. AGE-BSA or BSA was administered ip to normal mice twice a day for 2 wk. We showed that AGE-BSA-treated mice exhibited significantly higher glucose levels and lower insulin levels in response to glucose challenge than did BSA-treated mice, although there were no significant differences in insulin sensitivity and islet morphology between two groups. Glucose-stimulated insulin secretion by islets of the AGE-BSA-treated mice or AGE-BSA-treated normal islets was significantly lower than that by islets isolated from the BSA-treated mice or BSA-treated normal islets. Furthermore, AGE treatment of islet β-cells inhibited ATP production, and glimepiride, a sulfonylurea derivative, restored glucose-stimulated insulin secretion. Further investigation indicated that AGEs inhibited cytochrome c oxidase activity by inducing the expression of inducible nitric oxide synthase (iNOS). Blocking the formation of nitric oxide with an iNOS selective inhibitor aminoguanidine reversed the inhibitory effects of AGEs on ATP production and insulin secretion. We conclude that AGEs inhibit cytochrome c oxidase and ATP production, leading to the impairment of glucose-stimulated insulin secretion through iNOS-dependent nitric oxide production.

  C Zhao , W Zhuo , B Chen and H. Zhang
 

For calibration and intercomparison experiments, a thoron chamber with an inner volume of 300 l was designed based on a programmable constant temperature and humidity testing device in this work. The commercial lantern mantles enriched with 232Th were used as the 220Rn source and the mantles were set in 3x3x3 points of lattice style inside the chamber. Experimental studies showed that 220Rn concentrations in the chamber could be easily controlled and adjusted from about 0.5 to 80 kBq m–3 through manual settings of the relative humidity and temperature, and the spatial distribution of 220Rn in the chamber was fairly homogeneous.

  F Tang , W Zhuo , C Zhao , B Chen , Y Xu and L. He
 

For accurate measurements of 220Rn concentration with airflow-through scintillation cell method, a theoretical study was performed for discussing the influences of sampling flow rate, volumes of sampling tube and scintillation cell on the measurements. It is found that a high flow rate and a large inner volume of scintillation cell as well as a small inner volume of sampling tube are not only preferable for measuring low levels of 220Rn, but also helpful for enhancing the measurement accuracy. In calibration experiments, both the sampling flow rate and the sampling tube volume should be noted. The variations of the flow rate and tube volume should be considered for accurate measurements in the fields.

 
 
 
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